A simple and low-cost alternating current (AC)-based method, without electrolyte correction, is proposed (Electrochemical Impedance Spectroscopy (EIS)-Zero Gap Cell) for the determination of ohmic contribution of diaphragms. The effectiveness of the proposed methodology was evaluated by using a commercial Alkaline Water Electrolysis (AWE) diaphragm (Zirfon®). Furthermore, the results were compared with two conventional electrochemical methodologies for calculating the separator resistance, based on direct current (DC), and AC measurements, respectively. Compared with the previous techniques, the proposed approach reported more accurate and precise values of resistance for new and aged samples. Compared with the manufacturer reference, the obtained error values for new samples were 0.33%, 5.64%, and 41.7%, respectively for EIS-Zero gap cell, AC and DC methods, confirming the validity and convenience of the proposed technique.