Improved surface sensing of DNA on gas-etched porous silicon

Tessier, Daniel C.; Boughaba, S.; Arbour, M.; Roos, Pieter G.; Pan, Guohui

doi:10.1016/j.snb.2006.02.002

Cited by 28 publications

(16 citation statements)

References 35 publications

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“…Furthermore, the demonstration of in-situ DNA synthesis and measurement in porous silicon waveguides is noteworthy due to the size range of the pores in which the DNA was synthesized. Previous reports of DNA synthesis on glass [6,12] or silicon [13,14] supports have been on a much larger pore scale, often in the micron range.…”

Section: Single Nucleotide Sensingmentioning

confidence: 99%

Synthesis of DNA oligonucleotides in mesoporous silicon

Lawrie

Rong

et al. 2009

Physica Status Solidi (a)

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In‐situ oligonucleotide synthesis through an 8‐base sequence is demonstrated within 30 nm silicon mesopores utilizing sequential phosphoramidite reactions. Stepwise attachment of each base is confirmed by monitoring the porous silicon effective refractive index via a waveguide resonance angle. A linear increase in the effective refractive index is observed after the addition of each base, and the detection of oligonucleotides containing only a single thymine is possible. It is estimated that 50% of the available internal pore surface is occupied by the 8‐base oligonucleotides, which is a substantially greater coverage than obtained by using traditional methods of direct attachment of presynthesized DNA oligonucleotides. The greater surface coverage by DNA probes will enable more effective biosensing devices. (© 2009 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)

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Section: Single Nucleotide Sensingmentioning

confidence: 99%

Synthesis of DNA oligonucleotides in mesoporous silicon

Lawrie

Rong

et al. 2009

Physica Status Solidi (a)

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“…A number of techniques and methods to graft amines, oligonucleotides or related species on the PSi surface have been reported [86,[100][101][102][103][104][105][106]. The PSi surface could be thermally treated in ammonia containing atmosphere to obtain partial amine termination (∼8%) [107,108] or by wet chemical approaches [85,86,106].…”

Section: Biofunctionalization Of Psi Surfacementioning

confidence: 99%

“…Also, other approaches using PSi as a sensing material has been reported and PSi can be used in biomedical sensors detecting changes in electrical, optical and photoluminescence properties. These properties have been utilized; for example, in enzyme immobilization and protein capture studies [132,133], in biomolecular screening [134] and in DNA sensing [102,[135][136][137]. Thust et al used electrolyte-insulatorsemiconductor structures in their potentiometric biosensor to detect penicillinase, which was used as a model enzyme in the study.…”

Section: Biosensing With Psimentioning

confidence: 99%

Fabrication and chemical surface modification of mesoporous silicon for biomedical applications

Salonen

Lehto

2008

Chemical Engineering Journal

154

101

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“…The researchers have used tetramethylorthosilane, 3-aminopropyltriethoxy silane, tetraethyloxysilane, 2-(3, 4-epoxycyclohexyl)-ethyltrimethoxysilane, methyl-trimethoxy silane and 3-glycidoxypropyltrimethoxysilane (GOPS) [33,34], for immobilization of biomolecules for sensing application.…”

Section: Introductionmentioning

confidence: 99%

Peptide Nucleic Acid Immobilized Biocompatible Silane Nanocomposite Platform for Mycobacterium tuberculosis Detection

et al. 2010

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We have prepared nanocomposite films comprising of 3-glycidoxypropyltrimethoxysilane (GOPS) and iron-oxide (Fe 3 O 4 ) onto indium-tin-oxide (ITO) glass plate for covalent immobilization of 21-mer peptide nucleic acid (PNA). These films have been characterized using contact angle, atomic force microscopy (AFM), electrochemical techniques. The electrochemical response of the GOPS/ITO and Fe 3 O 4 -GOPS/ITO electrodes has been investigated by hybridization with complementary, non-complementary and one-base mismatch using methylene blue as electrochemical indicator. The PNA/Fe 3 O 4 -GOPS/ITO bioelectrode exhibits improved specificity and detection limit (0.1 fM) as compared to that of the PNA-GOPS/ITO bioelectrode (0.1 pM). This PNA/Fe 3 O 4 -GOPS/ITO electrode can be utilized for detection of hybridization with the complementary sequence in sonicated M. tuberculosis genomic DNA within 90 s of hybridization time.

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Improved surface sensing of DNA on gas-etched porous silicon

Cited by 28 publications

References 35 publications

Synthesis of DNA oligonucleotides in mesoporous silicon

Synthesis of DNA oligonucleotides in mesoporous silicon

Fabrication and chemical surface modification of mesoporous silicon for biomedical applications

Peptide Nucleic Acid Immobilized Biocompatible Silane Nanocomposite Platform for Mycobacterium tuberculosis Detection

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