Improved Survival of Embryonic Porcine Dopaminergic Neurons in Coculture with a Conditionally Immortalized GDNF-Producing Hippocampal Cell Line

Meyer, Morten; Johansen, Jens; Gramsbergen, Jan-Bert P.; Johansen, Teit E.; Zimmer, Jens

doi:10.1006/exnr.2000.7419

Cited by 35 publications

(21 citation statements)

References 74 publications

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“…GDNF expressing HiB5 cells continuously released high amounts of biologically active GDNF that in a subsequent study was shown to be sufficient to promote survival of dopaminergic neurons in co-culture with porcine ventral mesencephalic tissue. 17 To compare constitutive transgene expression to regulated expression for in vitro and in vivo use, we employed the tetracycline (Tet) inducible expression system. 7 The Tet system has gained widespread use for transgene regulation in gene transfer approaches (reviewed in Ref.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Tet-on system to avoid transgene down-regulation in ex vivo gene transfer to the CNS

et al. 2002

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Section: Discussionmentioning

confidence: 99%

Evaluation of Tet-on system to avoid transgene down-regulation in ex vivo gene transfer to the CNS

et al. 2002

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“…Organotypic brain slice cultures were prepared and grown by the interface culture method as also previously described3536. In brief, postnatal day 0–1 mice were quickly decapitated and the brains isolated under aseptic conditions.…”

Section: Methodsmentioning

confidence: 99%

Sensing of HSV-1 by the cGAS–STING pathway in microglia orchestrates antiviral defence in the CNS

et al. 2016

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Herpes simplex encephalitis (HSE) is the most common form of acute viral encephalitis in industrialized countries. Type I interferon (IFN) is important for control of herpes simplex virus (HSV-1) in the central nervous system (CNS). Here we show that microglia are the main source of HSV-induced type I IFN expression in CNS cells and these cytokines are induced in a cGAS–STING-dependent manner. Consistently, mice defective in cGAS or STING are highly susceptible to acute HSE. Although STING is redundant for cell-autonomous antiviral resistance in astrocytes and neurons, viral replication is strongly increased in neurons in STING-deficient mice. Interestingly, HSV-infected microglia confer STING-dependent antiviral activities in neurons and prime type I IFN production in astrocytes through the TLR3 pathway. Thus, sensing of HSV-1 infection in the CNS by microglia through the cGAS–STING pathway orchestrates an antiviral program that includes type I IFNs and immune-priming of other cell types.

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“…pigs [49] and human fetal brain tissue [50,51] have been used. Most organotypic brain slice cultures derive from early postnatal (P0-P7) animals, although attempts to culture adolescent or adult rat brain tissue have been recently made [52][53][54].…”

Section: Short History Of Disease Models Methods and Type Of Donor Smentioning

confidence: 99%

Organotypic Hippocampal Slice Cultures for Studies of Brain Damage, Neuroprotection and Neurorepair

Poulsen²,

et al. 2005

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Slices of developing brain tissue can be grown for several weeks as so-called organotypic slice cultures. Here we summarize and review studies using hippocampal slice cultures to investigate mechanisms and treatment strategies for the neurodegenerative disorders like stroke (cerebral ischemia), Alzheimer's disease (AD) and epilepsia. Studies of non-excitotoxic neurotoxic compounds and the experimental use of slice cultures in studies of HIV neurotoxicity, traumatic brain injury (TBI) and neurogenesis are included. For cerebral ischemia, experimental models with oxygen-glucose deprivation (OGD) and exposure to glutamate receptor agonists (excitotoxins) are reviewed. For epilepsia, focus is on induction of seizures with effects on neuronal loss, axonal sprouting and neurogenesis. For Alzheimer's disease, the review centers on the use of beta-amyloid (Abeta) in different models, while the section on repair is focused on neurogenesis and cell migration. The culturing techniques, set-up of models, and analytical tools, including markers for neurodegeneration, like the fluorescent dye propidium iodide (PI), are reviewed and discussed. Comparisons are made between hippocampal slice cultures and other in vitro models using dispersed cell cultures, experimental in vivo models, and in some instances, clinical trials. New techniques including slice culturing of hippocampal tissue from transgenic mice as well as more mature brain tissue, and slice cultures coupled to microelectrode arrays (MEAs), on-line biosensor monitoring, and time-lapse fluorescence microscopy are also presented.

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Improved Survival of Embryonic Porcine Dopaminergic Neurons in Coculture with a Conditionally Immortalized GDNF-Producing Hippocampal Cell Line

Cited by 35 publications

References 74 publications

Evaluation of Tet-on system to avoid transgene down-regulation in ex vivo gene transfer to the CNS

Evaluation of Tet-on system to avoid transgene down-regulation in ex vivo gene transfer to the CNS

Sensing of HSV-1 by the cGAS–STING pathway in microglia orchestrates antiviral defence in the CNS

Organotypic Hippocampal Slice Cultures for Studies of Brain Damage, Neuroprotection and Neurorepair

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