Improved survival through the reduction of ischemia-reperfusion injury after rat intestinal transplantation using selective P-selectin blockade with P-selectin glycoprotein ligand-Ig

Farmer, Douglas G.; Amersi, Farin; Shen, Xiu Da; Gao, Feng; Anselmo, Dean M.; Ma, Jeff; Dry, Sarah M.; McDiarmid, Sue V.; Shaw, Gray D.; Busuttil, Ronald W.; Kupiec‐Weglinski, Jerzy W.

doi:10.1016/s0041-1345(02)02730-6

Cited by 19 publications

(9 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…One such glycoprotein expressed on the surface of leukocytes is P-selectin glycoprotein ligand-1 (PSGL-1), which can bind all three selectins. 75,76 IR injury is a dynamic process involving an interaction of all of the pathophysiologic and molecular events, including production of reactive oxygen species, Kupffer and Ito cell activation, release of chemolymphocytokines and PMNs, and platelet and leukocyte adhesion. All of these in concert result in decreased sinusoidal blood flow, endothelial cell apoptosis, and necrosis, with eventual hepatocyte loss.…”

Section: Pathophysiology Of the Marginal Donormentioning

confidence: 99%

The utility of marginal donors in liver transplantation

Busuttil¹

2003

Liver Transplantation

630

508

View full text Add to dashboard Cite

The shortage of organs has led centers to expand their criteria for the acceptance of marginal donors. The combination of multiple marginal factors seems to be additive on graft injury. In this review, the utility of various marginal donors in patients requiring liver transplantation will be described, including older donors, steatotic livers, non-heart-beating donors, donors with viral hepatitis, and donors with malignancies. The pathophysiology of the marginal donor will be discussed, along with strategies for minimizing the ischemia reperfusion injury experienced by these organs. Finally, new strategies for improving the function of the marginal/expanded donor liver will be reviewed. (Liver Transpl 2003;9:651-663.)

show abstract

Section: Pathophysiology Of the Marginal Donormentioning

confidence: 99%

The utility of marginal donors in liver transplantation

Busuttil¹

2003

Liver Transplantation

630

508

View full text Add to dashboard Cite

show abstract

“…During reperfusion, however, the accumulated hypoxanthine is transformed by the xanthine oxidase, in the presence of molecular oxygen, into free radicals of oxygen: superoxides, peroxides, and hydroxysl 6,7,8 , resulting in a systemic inflammatory process characterized by the increase in endothelial permeability to fluids, macromolecules, and inflammatory cells 6,9,10 .The deleterious effects of reperfusion surpass the effects of the actual ischemia, since the metabolic toxins accumulated during the period of ischemia will be distributed throughout other organs 1,11,12,13 . In the intestine, IR results in tissue lesion and morphological alterations, compromising the absorptive function 14 .…”

Section: Introductionmentioning

confidence: 99%

The protective effect of methylene blue in lungs, small bowel and kidney after intestinal ischemia and reperfusion

et al. 2008

View full text Add to dashboard Cite

PURPOSE: To study the role of methylene blue as an inhibitor of superoxide production by xanthine oxidase. METHODS: Thirty-two Wistar rats were divided into 2 groups of 16 animals: the control group and the experimental group. All were submitted to a laparotomy for the occlusion of the cranial mesenteric artery during 60 minutes. The reperfusion was confirmed by the pulsation of the artery after the release of the temporary ligature and color change of the intestines. In the animals of the control group, 2 ml of saline were injected in the peritoneal cavity and in the animals of the experimental group, 2 ml of methylene blue were injected in the peritoneal cavity. After reperfusion for 4 hours, the animals were then sacrificed. The lungs were excised from all 32 rats. Simultaneously, the small intestine and kidneys were ressected in 20 animals (10 from the control group and 10 from the experimental group). Samples of the organs were taken to evaluate the action of xanthine-oxidase, for histopathology studies and for characterization of the edema. RESULTS: In the animals of the experimental group, the inflammatory lesion as well as the edema in the lung was greater than in the control group. The intestinal and renal lesions were similar in both groups, but the lung damage was superior to that observed in the intestines and kidneys. . CONCLUSION: Despite similar action of the xanthine oxidase in the control and the experimental group, after intestinal ischemia and reperfusion, the protective effect of methylene blue was observed only in the lungs of the experimental group.

show abstract

“…P-selectin, an adhesion molecule expressed by activated endothelial cells, mediates the early phases of leukocyte adherence to the endothelium. Expression of P-selectin has been shown to be crucial to neutrophil recruitment in many human inflammatory processes [9] as well as in animal models of intestinal ischemia-reperfusion, intestinal transplantation, and sepsis [10][11][12][13][14][15], but its role in NEC is unknown.…”

mentioning

confidence: 99%