Improved technique of heterotopic auxiliary rat liver transplantation with portal vein arterialization

Schleimer, Karina; Stippel, Dirk L.; Tawadros, Samir; Hölzen, Jens Peter; Hölscher, Arnulf H.; Beckurts, K. T. E.

doi:10.1007/s00423-006-0032-x

Cited by 8 publications

(10 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Subsequent to this, various ALT models in rats have been developed ( 15 – 17 ). Of these models, the APHLT with PVA model in rats was developed by Schleimer et al ( 7 ); one of advantages of this model is that portal venous blood flow is controlled by the connected stent. This model is simple and easy to perform.…”

Section: Discussionmentioning

confidence: 99%

“…For these reasons, several clinical studies using APHLT with PVA have been conducted in recent years ( 4 – 6 ); however, the graft liver has been found to fail in long-term survival. To solve this problem, Schleimer et al developed a rat model of APHLT with PVA ( 7 ). In this model, the graft portal vein is connected by a stent with 0.3 mm inner diameter to the recipient’s right renal artery.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A new rat model of auxiliary partial heterotopic liver transplantation with liver dual arterial blood supply

Qiao

Han

Zhang

et al. 2014

Experimental and Therapeutic Medicine

View full text Add to dashboard Cite

Auxiliary partial heterotopic liver transplantation (APHLT) with portal vein arterialization is a valuable procedure to be considered in the treatment of patients with acute liver failure and metabolic liver diseases. The aim of this study was to develop a new rat model of APHLT with liver dual arterial blood supply (LDABS). A total of 20 rats were used. The donor liver was resected, and the celiac trunk was reserved. Left and medial hepatic lobes accounting for 70% of the liver mass were removed en bloc and the suprahepatic caval vein was ligated simultaneously. Thus, 30% of the donor liver was obtained as the graft. Sleeve anastomosis of the graft portal vein and splenic artery were performed after narrowing the portal vein lumen through suturing. The right kidney of the recipient was removed, and sleeve anastomosis was performed between the celiac trunk of the graft and the right renal artery of the recipient. In addition, end-to-end anastomosis was performed between the infrahepatic caval vein of the graft and the right renal vein of the recipient. Following the reperfusion of the graft, the blood flow of the arterialized portal vein was controlled within the physiological range through suturing and narrowing under monitoring with an ultrasonic flowmeter. The bile duct of the graft was implanted into the duodenum of the recipient through an internal stent catheter. A 70% section of the native liver (left and medial hepatic lobes) was resected using bloodless hepatectomy. The mean operative duration was 154.5±16.4 min, and the warm and cold ischemia times of the graft were 8.1±1.1 min and 64.5±6.6 min, respectively. The blood flow of the arterialized portal vein to the graft was 1.8±0.3 ml/min/g liver weight. The success rate of model establishment (waking with post-surgical survival of >24 h) was 70% (7/10). Following successful model establishment, all rats survived 7 days post-surgery (100%; 7/7). The graft was found to be soft in texture and bright red in color following exploratory laparotomy. In conclusion, a new rat model of APHLT with LDABS without stent for vascular reconstruction was developed. This is a feasible and reliable rat model for liver transplantation study.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A new rat model of auxiliary partial heterotopic liver transplantation with liver dual arterial blood supply

Qiao

Han

Zhang

et al. 2014

Experimental and Therapeutic Medicine

View full text Add to dashboard Cite

show abstract

Section: Methodsmentioning

confidence: 99%

“…Heterotopic auxiliary and reduced (30% of the total volume) rat liver transplantation with portal vein arterialization was performed in adult, male Sprague Dawley rats using a described technique. (23) The engraftment was done without performing the hepatic artery anastomosis. Macroscopy appearance, color, and consistency of the engrafted livers after reperfusion was identical to the native livers ( Fig.…”

Section: Surgery and Experimental Designmentioning

confidence: 99%

The Novel N,N‐bis‐2‐Hydroxyethyl‐2‐Aminoethanesulfonic Acid–Gluconate–Polyethylene Glycol–Hypothermic Machine Perfusion Solution Improves Static Cold Storage and Reduces Ischemia/Reperfusion Injury in Rat Liver Transplant

et al. 2019

View full text Add to dashboard Cite

Organ transplantation is the treatment of choice against terminal and irreversible organ failure. Optimal preservation of the graft is crucial to counteract cold ischemia effects. As we developed an N,N‐bis‐2‐hydroxyethyl‐2‐aminoethanesulfonic acid–gluconate–polyethylene glycol (BGP)–based solution (hypothermic machine perfusion [HMP]), we aimed to analyze the use of this solution on static cold storage (SCS) of rat livers for transplantation as compared with the histidine tryptophan ketoglutarate (HTK) preservation solution. Livers procured from adult male Sprague Dawley rats were preserved with BGP‐HMP or HTK solutions. Liver total water content and metabolites were measured during the SCS at 0°C for 24 hours. The function and viability of the preserved rat livers were first assessed ex vivo after rewarming (90 minutes at 37°C) and in vivo using the experimental model of reduced‐size heterotopic liver transplantation. After SCS, the water and glycogen content in both groups remained unchanged as well as the tissue glutathione concentration. In the ex vivo studies, livers preserved with the BGP‐HMP solution were hemodynamically more efficient and the O2 consumption rate was higher than in livers from the HTK group. Bile production and glycogen content after 90 minutes of normothermic reperfusion was diminished in both groups compared with the control group. Cellular integrity of the BGP‐HMP group was better, and the histological damage was reversible. In the in vivo model, HTK‐preserved livers showed a greater degree of histological injury and higher apoptosis compared with the BGP‐HMP group. In conclusion, our results suggest a better role of the BGP‐HMP solution compared with HTK in preventing ischemia/reperfusion injury in the rat liver model.

show abstract

“…The animal models in groups A and B were prepared based on methods previously described ( 4 , 7 ). The animal model in group C was established on the basis of an improvement of the methods described by Schleimer et al ( 8 ). In brief, the recipient underwent a 68% liver and right kidney resection.…”

Section: Methodsmentioning

confidence: 99%

Effects of dual arterial blood supply on liver regeneration in the graft and the host following heterotopic auxiliary liver transplantation

Zhang

Dong

et al. 2014

Experimental and Therapeutic Medicine

View full text Add to dashboard Cite

This study aimed to investigate the effect of the dual arterial blood supply method used in auxiliary liver transplantation on the regeneration of grafted and host liver. A total of 72 male Sprague-Dawley rats were randomly assigned to three experimental groups, namely the 68% hepatectomy group (group A), the 68% hepatectomy with dual arterial blood supply group (group B) and the auxiliary liver transplantation with dual arterial blood supply group (group C). Group C was further divided into the host liver subgroup (group Ca) and the transplanted liver subgroup (group Cb). Six animals from each group were sacrificed at 1, 2 and 7 days after surgery. The calculation of the liver regeneration rate (LRR) was based on measuring liver weight. Liver function was assessed by measuring serum alanine aminotransferase (ALT) levels. Immunohistochemistry was employed to detect the expression of proliferating cell nuclear antigen (PCNA). Apoptotic changes in the grafts and host livers were evaluated using TUNEL staining. The LRR in each group exhibited a tendency to increase over time. At each time point, the LRR of transplanted livers in group C exhibited no significant difference from that of host livers in group C (P>0.05). The ALT levels for each group exhibited a time-dependent decreasing tendency. The ALT level in group C was significantly higher compared to that in groups A and B at each time point (P<0.05). The expression of PCNA in transplanted and host livers in group C was significantly lower compared to that in groups A and B at the same time point (P<0.001). Although the number of apoptotic cells in each group varied at different time points, there was no statistically significant difference (P>0.05). In auxiliary liver transplantation with the dual arterial blood supply method, the capacity of the liver regeneration in the grafts was similar to that of the host livers. Therefore, this technique may reduce the potential risk of graft liver atrophy caused by functional competition.

show abstract

Improved technique of heterotopic auxiliary rat liver transplantation with portal vein arterialization

Cited by 8 publications

References 16 publications

A new rat model of auxiliary partial heterotopic liver transplantation with liver dual arterial blood supply

A new rat model of auxiliary partial heterotopic liver transplantation with liver dual arterial blood supply

The Novel N,N‐bis‐2‐Hydroxyethyl‐2‐Aminoethanesulfonic Acid–Gluconate–Polyethylene Glycol–Hypothermic Machine Perfusion Solution Improves Static Cold Storage and Reduces Ischemia/Reperfusion Injury in Rat Liver Transplant

Effects of dual arterial blood supply on liver regeneration in the graft and the host following heterotopic auxiliary liver transplantation

Contact Info

Product

Resources

About