Improved techniques for the separation of serum lipoproteins by density gradient ultracentrifugation: Visualization by prestaining and rapid separation of serum lipoproteins from small volumes of serum

Terpstra, A.H.M.; Woodward, Christopher J. H.; Sánchez‐Muniz, Francisco J.

doi:10.1016/0003-2697(81)90243-8

Cited by 361 publications

(105 citation statements)

References 12 publications

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“…To reduce interassay variation of TC, triglycerides and lipoprotein lipid measurements were performed after the completion of the experiment by using aliquots stored at 770 C. Apo determinations were performed in fresh samples. Lipid determinations from very low density lipoprotein (VLDL), LDL or HDL fractions were performed after their isolation from serum by 21 h density gradient ultracentrifugation at 40 000 rpm (272 000 g) and 8 C in a Beckman L8-70M ultracentrifuge, SW-41 rotor (Palo Alto, California), following the method of Terpstra et al (1981). All determinations were carried out in duplicate.…”

Section: Laboratory Analysesmentioning

confidence: 99%

Lipoprotein profiles and serum peroxide levels of aged women consuming palmolein or oleic acid-rich sunflower oil diets

et al. 1998

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Objective: To investigate the hypercholesterolemic effects of a dietary exchange between 16:0 and 18:1 while 18:2 was at relatively lower level ( % 4%) in aged women with initially high total serum cholesterol (TC) and low density lipoprotein cholesterol (LDL-C) values and with high intakes of dietary cholesterol. Design: Subjects were assigned to two consecutive 28 d periods. In the ®rst period all subjects followed an oleic acid-rich diet in the form of oleic acid-rich sun¯ower oil. This was followed by a second period rich in palmitic acid in the form of palmolein. Nutrient intakes, serum lipids, lipoproteins, antioxidant vitamins, peroxides and LDL-peroxides were measured at two dietary periods. Setting: Instituto de Nutricio Ân y Bromatologõ Âa (CSIC), Departamento de Nutricio Ân y Bromatologõ Âa I (Nutricio Ân) and Seccio Ân Departamental de Quõ Âmica Analõ Âtica, Universidad Complutense, Madrid, Spain. Results: The palmolein period led to an increase in TC (P`0.001; 17.7%) and serum apolipoprotein (Apo) B levels (P`0.001; 18.0%). LDL-C and LDL-Apo B concentrations were higher (P`0.001, 4.33AE 0.94 mmolaL and P`0.01, 1.08 AE 0.20 gaL, respectively) following this period than following the oleic acid-rich sun¯ower oil diet (3.56 AE 0.85 mmolaL, 0.93 AE 0.16 gaL, respectively). No signi®cant differences were observed in the TCahigh density lipoprotein cholesterol (TCaHDL-C) ratio between the two dietary periods. Serum and LDLperoxides were lower (P`0.01, 49.5%, and P`0.001, 69.0%, respectively) after the palmolein diet than after the oleic acid-rich sun¯ower oil diet. The palmolein diet signi®cantly increased TC, LDL-C, Apo B, VLDLApoB, LDL-ApoB in women with TC ! 6.21 mmolaL or with TC`6.21 mmolaL, but the increase in Apo B, LDL-C and LDL-Apo B was greater among the women with high TC. The palmolein diet increased HDL-C in women with high or with low TC but this rise was on the borderline of statistical signi®cance (P 0.06) only in normocholesterolemics. Serum and LDL-peroxides tended to be higher in women with TC ! 6.21 mmolaL than in women with TC`6.21 mmolaL, but palmolein decreased serum and LDL-peroxide in hypercholesterolemics more than in the normocholesterolemics, resulting in serum and LDL-peroxide levels which theoretically are more adequate. Conclusions: Though palmolein increased LDL-C concentrations, it better protected LDL particles, mainly in women with high TC, against peroxidation than did oleic acid-rich sun¯ower oil. Sponsorship: This study was supported by the Spanish Comision Interministerial de Ciencia Y Tecnologõ Âa

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Section: Laboratory Analysesmentioning

confidence: 99%

Lipoprotein profiles and serum peroxide levels of aged women consuming palmolein or oleic acid-rich sunflower oil diets

et al. 1998

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“…The following fractions were sequentially isolated from the top of the tube: very low-density lipoproteins (VLDLs, fraction 1), low-density lipoproteins (LDLs, fraction 2), Cremophor with unbound GePcs (fraction 3), high-density lipoproteins (HDLs, fraction 4), heavy proteins, including mainly albumin and globulins (fraction 5). The identification of the fractions was performed as described elsewhere (Terpstra et al, 1981). The volume of each fraction was measured, then the fractions were dialysed overnight against physiological solution, diluted with a binary solvent mixture (0.4 ml of 4% aqueous SDS plus 1.5 ml of tetrahydrofuran) and the GePc content was determined using the spectrophotofluorimeter.…”

Section: Animals and Tumourmentioning

confidence: 99%

Effect of axial ligation and delivery system on the tumour-localising and -photosensitising properties of Ge(IV)-octabutoxy-phthalocyanines

Soncin

Polo²,

Reddi³

et al. 1995

Br J Cancer

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Summary Four Ge(IV)-octabutoxy-phthalocyanines (GePcs) bearing two alkyl-type axial ligands were assayed for their pharmacokinetic properties and phototherapeutic efficiency in Balb/c mice bearing an intramuscularly transplanted MS-2 fibrosarcoma. The GePcs were i.v. injected at a dose of 0.351imolkg-' body weight after incorporation into either Cremophor emulsions or small unilamellar liposomes of dipalmitoyl-phosphatidylcholine (DPPC). Both the nature of the delivery system and the chemical structure of the phthalocyanine were found to affect the behaviour of the GePcs in vivo. Thus, Cremophor-administered GePcs invariably yielded a more prolonged serum retention and a larger association with low-density lipoproteins (LDLs) as compared with the corresponding liposome-delivered phthalocyanines. This led to a greater efficiency and selectivity of tumour targeting. These effects were more pronounced for those GePcs having relatively long alkyl chains (hexyl to decyl) in the axial ligands. Maximal tumour accumulation (0.67 nmol per g of tissue) was found for Ge-Pc(hexyl)2 at 24 h after injection. Consistently, the Ge-Pc(hexyl)2, administered via Cremophor, showed the highest phototherapeutic activity towards the MS-2 fibrosarcoma.

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“…The method of Terpstra et al (1981) was used for separating lipoproteins by ultracentrifugation. Plasma samples were stored at 4°C for periods of up to two days.…”

Section: Sampling Of Blood and Analytical Methodsmentioning

confidence: 99%

Plasma lipoprotein profiles and arylesterase activities in two inbred strains of rabbits with high or low response of plasma cholesterol to dietary cholesterol

Beynen

Katan

Zutphen

1984

Comparative Biochemistry and Physiology Part B: Comparative Bio

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Abstract--1. Cholesterol feeding for 4 weeks of female and male rabbits of two inbred strains increased plasma cholesterol concentrations by about 11 and 48 mmole/I in the hypo-and hyperresponsive strain, respectively.2. On the low-cholesterol pre-experimental diet, the hyporesponsive animals had significantly higher plasma HDL (high density protein) cholesterol levels than hyperresponders.3. In both strains, cholesterol feeding caused elevations of cholesterol in all lipoprotein classes, the difference between the hypo-and hyperresponsive strains in essence only being observed in the VLDL (very low density lipoprotein) fraction.4. Basal plasma total arylesterase activity was significantly higher in the hypo-than in the hyperresponsive rabbits.5. Dietary cholesterol caused an increase in plasma esterase activity in both strains. 6. We suggest that in rabbits a low plasma arylesterase activity and a low concentration of HDL cholesterol are associated with an increased sensitivity to dietary cholesterol.

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Improved techniques for the separation of serum lipoproteins by density gradient ultracentrifugation: Visualization by prestaining and rapid separation of serum lipoproteins from small volumes of serum

Cited by 361 publications

References 12 publications

Lipoprotein profiles and serum peroxide levels of aged women consuming palmolein or oleic acid-rich sunflower oil diets

Lipoprotein profiles and serum peroxide levels of aged women consuming palmolein or oleic acid-rich sunflower oil diets

Effect of axial ligation and delivery system on the tumour-localising and -photosensitising properties of Ge(IV)-octabutoxy-phthalocyanines

Plasma lipoprotein profiles and arylesterase activities in two inbred strains of rabbits with high or low response of plasma cholesterol to dietary cholesterol

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