Improved thermostability and cytocompatibility of bacterial cellulose/collagen composite by collagen fibrillogenesis

Dai, Lei; Nan, Jie; Tu, Xiao; He, Lang; Wei, Benmei; Xu, Chen; Xu, Yong; Li, Sheng; Wang, Haibo; Zhang, Juntao

doi:10.1007/s10570-019-02530-w

Cited by 24 publications

(8 citation statements)

References 37 publications

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“…Additionally, it was established that the impregnation of alginate in BC allowed to improve its water retention [ 11 ], and it was found that the addition of beeswax into BC film was possible to increase its hydrophobicity by confirming its contact angle [ 12 ]. It was also confirmed that the incorporation of collagen between bacterial cellulosic fibers allowed the fabricated BC-based materials to enhance thermal stability as well as its cytocompatibility by collagen fibrillogenesis [ 13 ].…”

Section: Introductionmentioning

confidence: 95%

Bacterial Cellulose and Its Applications

et al. 2022

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The sharp increase in the use of cellulose seems to be in increasing demand in wood; much more research related to sustainable or alternative materials is necessary as a lot of the arable land and natural resources use is unsustainable. In accordance, attention has focused on bacterial cellulose as a new functional material. It possesses a three-dimensional, gelatinous structure consisting of cellulose with mechanical and thermal properties. Moreover, while a plant-originated cellulose is composed of cellulose, hemi-cellulose, and lignin, bacterial cellulose attributable to the composition of a pure cellulose nanofiber mesh spun is not necessary in the elimination of other components. Moreover, due to its hydrophilic nature caused by binding water, consequently being a hydrogel as well as biocompatibility, it has only not only used in medical fields including artificial skin, cartilage, vessel, and wound dressing, but also in delivery; some products have even been commercialized. In addition, it is widely used in various technologies including food, paper, textile, electronic and electrical applications, and is being considered as a highly versatile green material with tremendous potential. However, many efforts have been conducted for the evolution of novel and sophisticated materials with environmental affinity, which accompany the empowerment and enhancement of specific properties. In this review article, we summarized only industry and research status regarding BC and contemplated its potential in the use of BC.

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Section: Introductionmentioning

confidence: 95%

Bacterial Cellulose and Its Applications

et al. 2022

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“…These individual nanofibrils are further assembled to form highly oriented microfibrils or even fiber bundles, conferring exceptional mechanical properties to biological materials. Because of its structural similarity to the components of the extracellular matrix (e.g., collagen), BC is capable of interacting with biological tissues [ 13 , 14 ]. This complex of beneficial properties makes BC a promising biomaterial for the development of biocompatible matrices for tissue engineering [ 15 , 16 , 17 , 18 , 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

Chitosan Composites with Bacterial Cellulose Nanofibers Doped with Nanosized Cerium Oxide: Characterization and Cytocompatibility Evaluation

Петрова

Gofman

Dubashynskaya

et al. 2023

IJMS

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In this work, new composite films were prepared by incorporating the disintegrated bacterial cellulose (BCd) nanofibers and cerium oxide nanoparticles into chitosan (CS) matrices. The influence of the amount of nanofillers on the structure and properties of the polymer composites and the specific features of the intermolecular interactions in the materials were determined. An increase in film stiffness was observed as a result of reinforcing the CS matrix with BCd nanofibers: the Young’s modulus increased from 4.55 to 6.3 GPa with the introduction of 5% BCd. A further increase in Young’s modulus of 6.7 GPa and a significant increase in film strength (22% increase in yield stress compared to the CS film) were observed when the BCd concentration was increased to 20%. The amount of nanosized ceria affected the structure of the composite, followed by a change in the hydrophilic properties and texture of the composite films. Increasing the amount of nanoceria to 8% significantly improved the biocompatibility of the films and their adhesion to the culture of mesenchymal stem cells. The obtained nanocomposite films combine a number of favorable properties (good mechanical strength in dry and swollen states, improved biocompatibility in relation to the culture of mesenchymal stem cells), which allows us to recommend them for use as a matrix material for the culture of mesenchymal stem cells and wound dressings.

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“…Some collagen-based/BC composites have already been prepared following different methods while targeting diverse final uses such as in biomedical applications, − tissue regeneration, , or tissue-engineered scaffolds. − For instance, collagen has already been incorporated into BC films by immersion, − ,, which can be mediated by Fmoc-glycine (Fmoc-Gly) esterification, by adding collagen to the bacterial culture medium, or by diffusion under an electric field . The evaluation of BC films incorporating other biomaterials (hyaluronic acid, silk) indicates increased tensile strength.…”

Section: Introductionmentioning

confidence: 99%

Cell-Laden 3D Hydrogels of Type I Collagen Incorporating Bacterial Nanocellulose Fibers

Malandain,

Sanz-Fraile,

Farré

et al. 2023

ACS Appl. Bio Mater.

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There is a growing interest in developing natural hydrogel-based scaffolds to culture cells in a three-dimensional (3D) millieu that better mimics the in vivo cells' microenvironment. A promising approach is to use hydrogels from animal tissues, such as decellularized extracellular matrices; however, they usually exhibit suboptimal mechanical properties compared to native tissue and their composition with hundreds of different protein complicates to elucidate which stimulus triggers cell's responses. As simpler scaffolds, type I collagen hydrogels are used to study cell behavior in mechanobiology even though they are also softer than native tissues. In this work, type I collagen is mixed with bacterial nanocellulose fibers (BCf) to develop reinforced scaffolds with mechanical properties suitable for 3D cell culture. BCf were produced from blended pellicles biosynthesized from Komagataeibacter xylinus. Then, BCf were mixed with concentrated collagen from rat-tail tendons to form composite hydrogels. Confocal laser scanning microscopy and scanning electron microscopy images confirmed the homogeneous macro-and microdistribution of both natural polymers. Porosity analysis confirmed that BCf do not disrupt the scaffold structure. Tensile strength and rheology measurements demonstrated the reinforcement action of BCf (43% increased stiffness) compared to the collagen hydrogel while maintaining the same viscoelastic response. Additionally, this reinforcement of collagen hydrogels with BCf offers the possibility to mix cells before gelation and then proceed to the culture of the 3D cell scaffolds. We obtained scaffolds with human bone marrow-derived mesenchymal stromal cells or human fibroblasts within the composite hydrogels, allowing a homogeneous 3D viable culture for at least 7 days. A smaller surface shrinkage in the reinforced hydrogels compared to type I collagen hydrogels confirmed the strengthening of the composite hydrogels. These collagen hydrogels reinforced with BCf might emerge as a promising platform for 3D in vitro organ modeling, tissue-engineering applications, and suitable to conduct fundamental mechanobiology studies.

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Improved thermostability and cytocompatibility of bacterial cellulose/collagen composite by collagen fibrillogenesis

Cited by 24 publications

References 37 publications

Bacterial Cellulose and Its Applications

Bacterial Cellulose and Its Applications

Chitosan Composites with Bacterial Cellulose Nanofibers Doped with Nanosized Cerium Oxide: Characterization and Cytocompatibility Evaluation

Cell-Laden 3D Hydrogels of Type I Collagen Incorporating Bacterial Nanocellulose Fibers

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