Improved versions of rice maintainer line, APMS 6B, possessing two resistance genes, Xa21 and Xa38, exhibit high level of resistance to bacterial blight disease

Yugander, A.; Sundaram, R. M.; Singh, Kuldeep; Senguttuvel, P.; Ladhalakshmi, D.; Kemparaju, K. B.; Madhav, M. S.; Prasad, M. S.; Hariprasad, A. S.; Laha, G. S.

doi:10.1007/s11032-018-0853-7

Cited by 13 publications

(13 citation statements)

References 42 publications

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“…In addition, the results suggest that the gene combinations containing the Xa21 gene were most resistant, as evidenced by shorter lesions lengths, followed by Xa4 + Xa21, Xa7 + Xa21 and xa13 + Xa21, while lines with Xa4 + xa5, xa5 + xa13 and Xa7 + xa13 were less effective. These results are consistent with previous studies, which have shown the presence of Xa21 to be correlated with high levels of persistent resistance against BB disease in rice [6,14,15,17,25]. Xa21 is the cell surface receptor, kinase, which is able to provide resistance to Xoo infections; Xa21 not only suppresses Xoo growth, but also triggers broad perturbation in rice transcriptomes and mediated signaling pathways, preventing Xoo infections [14].…”

Section: Discussionsupporting

confidence: 92%

“…Conventional backcross breeding is the primary method used to develop highly BB-resistant rice cultivars, but it cannot accurately transfer multiple genes into the cultivar by phenotypic screening and the process requires a significant amount of time [21,22]. Modified backcross pyramid breeding, combined with molecular marker-assisted selection, has already been demonstrated to increase the precision and efficiency of breeding [23][24][25]. Due to the relatively large amount of work involved with the MAS process, the conventional backcross breeding approach has been widely adopted in breeding programs designed to breed for BB resistance [10,[26][27][28].…”

Section: Discussionmentioning

confidence: 99%

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Pyramiding Bacterial Blight Resistance Genes in Tainung82 for Broad-Spectrum Resistance Using Marker-Assisted Selection

Hsu

Chiu²,

Yap

et al. 2020

IJMS

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Tainung82 (TNG82) is one of the most popular japonica varieties in Taiwan due to its relatively high yield and grain quality, however, TNG82 is susceptible to bacterial blight (BB) disease. The most economical and eco-friendly way to control BB disease in japonica is through the utilization of varieties that are resistant to the disease. In order to improve TNG82’s resistance to BB disease, five bacterial blight resistance genes (Xa4, xa5, Xa7, xa13 and Xa21) were derived from a donor parent, IRBB66 and transferred into TNG82 via marker-assisted backcrossing breeding. Five BB-resistant gene-linked markers were integrated into the backcross breeding program in order to identify individuals possessing the five identified BB-resistant genes (Xa4, xa5, Xa7, xa13 and Xa21). The polymorphic markers between the donor and recurrent parent were used for background selection. Plants having maximum contribution from the recurrent parent genome were selected in each generation and crossed with the recipient parent. Selected BC3F1 plants were selfed in order to generate homozygous BC3F2 plants. Nine pyramided plants, possessing all five BB-resistant genes, were obtained. These individuals displayed a high level of resistance against the BB strain, XF89-b. Different BB gene pyramiding lines were also inoculated against the BB pathogen, resulting in more than three gene pyramided lines that exhibited high levels of resistance. The five identified BB gene pyramided lines exhibited yield levels and other desirable agronomic traits, including grain quality and palatability, consistent with TNG82. Bacterial blight-resistant lines possessing the five identified BB genes exhibited not only higher levels of resistance to the disease, but also greater yield levels and grain quality. Pyramiding multiple genes with potential characteristics into a single genotype through marker-assisted selection can improve the efficiency of generating new crop varieties exhibiting disease resistance, as well as other desirable traits.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Pyramiding Bacterial Blight Resistance Genes in Tainung82 for Broad-Spectrum Resistance Using Marker-Assisted Selection

Hsu

Chiu²,

Yap

et al. 2020

IJMS

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“…GU-2 is a Near Isogenic Line of APMS6B, developed through marker assisted backcross breeding of two bacterial blight resistance genes viz., Xa21 and Xa38 to confer broad spectrum resistance to bacterial blight (Yugander et al 2018) and has medium slender grain and excellent cooking quality which was used as donor parent for bacterial leaf blight disease. The parental lines viz., APMS6B, GU-2, RPHR 1005 (male parent of DRRH-3) and hybrid DRRH-3 were intolerant to low soil P.…”

Section: Donor For Bacterial Blightmentioning

confidence: 99%

“…Neither the CMS line (APMS6B) and the restorer line (RPHR 1005) nor does the derived hybrid (DRRH-3) acquire tolerance to low phosphorus and resistance to BB. In previous study, the improved maintainer line APMS6B of DRRH-3 known as GU-2, was developed by incorporating two bacterial leaf blight (BB) resistance genes (Xa21 and Xa38), through marker assisted backcross breeding (MABB) approach (Yugander et al 2018). The scope of marker-assisted breeding for targeted introgression of BB resistance genes (Yugander et al 2018;Sundaram et al 2008;Amante-Bordeos et al 1992;Hittalmani et al 2000) and Pup1 (Swamy et al 2020;Anila et al 2018;Chin et al 2011;Chithrameenal et al 2018) has been successfully demonstrated.…”

Section: Introductionmentioning

confidence: 99%

“…In previous study, the improved maintainer line APMS6B of DRRH-3 known as GU-2, was developed by incorporating two bacterial leaf blight (BB) resistance genes (Xa21 and Xa38), through marker assisted backcross breeding (MABB) approach (Yugander et al 2018). The scope of marker-assisted breeding for targeted introgression of BB resistance genes (Yugander et al 2018;Sundaram et al 2008;Amante-Bordeos et al 1992;Hittalmani et al 2000) and Pup1 (Swamy et al 2020;Anila et al 2018;Chin et al 2011;Chithrameenal et al 2018) has been successfully demonstrated. In all these studies, either BB resistance or low P tolerant genes (any two traits) were introgressed separately into commercial varieties.…”

Section: Introductionmentioning

confidence: 99%

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Stacking of Pup1 QTL for low soil phosphorus tolerance and bacterial blight resistance genes in the background of APMS6B, the maintainer line of rice hybrid DRRH-3

et al. 2022

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Phosphorus (P) is one of the macro nutrients essential for plant growth and development. Rice (Oryza sativa L.) is sensitive to P starvation and its deficiency influences many key plant functions which results in crop yield penalty.Although the hybrid rice segment is well-known for its yield heterosis, P deficiency and bacterial leaf blight (BLB) diseases are the evident limitations. APMS6B, the female parent of DRRH-3 is susceptible to low P and bacterial blight disease. In the present study, the improvement of APMS6B to P starvation and resistance to bacterial leaf blight (BB) was carried out using marker-assisted backcross breeding (MABB) approach. Kasalath (+ Pup1 QTL) was used as donor and a promising IL (ATR 594-1) at BC1F4 generation was identified with 81.15% RPGR.Concurrently, this IL was intercrossed with GU-2 (+ Xa21 and Xa38). Hybridity of Intercross F1s (ICF1) was confirmed through foreground selection having maximum RPGR (88.29%) and were selfed to produce ICF2. The resultant progenies were phenotyped for BB using Xoo inoculum (IX-020), simultaneously genotyped with gene specific functional SSR markers for Xa21 and Xa38. The identified BB resistant plants were subjected to foreground selection for Pup1. Four promising ICF3 plants (BP-10-1, BP-10-3, BP-10-5 and BP-10-15 with Xa21, Xa38 and Pup1) along with parents and checks were screened both in low P plot (<2 kg P2O5 ha -1 ) as well as in normal plot (>25 kg P2O5 ha -1 ) during dry and wet seasons 2018. Based on the field evaluation, four promising intercrossed lines were identified with better root architecture in terms of root length and root volume. In addition, less % reduction in grain yield (39.10%) under P starvation and less susceptibility indices values (<1) for BB were observed. These lines may be utilized in the CMS conversion programme and development of climate resilient, biotic and abiotic tolerant rice hybrids.

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Weed Science and Weed Management in Rice and Cereal‐Based Cropping Systems

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Improved versions of rice maintainer line, APMS 6B, possessing two resistance genes, Xa21 and Xa38, exhibit high level of resistance to bacterial blight disease

Cited by 13 publications

References 42 publications

Pyramiding Bacterial Blight Resistance Genes in Tainung82 for Broad-Spectrum Resistance Using Marker-Assisted Selection

Pyramiding Bacterial Blight Resistance Genes in Tainung82 for Broad-Spectrum Resistance Using Marker-Assisted Selection

Stacking of Pup1 QTL for low soil phosphorus tolerance and bacterial blight resistance genes in the background of APMS6B, the maintainer line of rice hybrid DRRH-3

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