Improvement and characterization of surfactant-modified Prussian blue screen-printed carbon electrodes for selective H2O2 detection at low applied potentials

Salazar, Pedro; Martín, Miriam; O’Neill, Robert; Roche, R.; González–Mora, José Luis

doi:10.1016/j.jelechem.2012.04.005

Cited by 48 publications

(18 citation statements)

References 43 publications

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“…All experiments were done in a 20 mL glass cell containing PBS (pH 7.4) at an ambient 21 ± 1 °C, using a standard three-electrode set-up, including a silver pseudo-reference electrode (250 µm diameter Ag wire) whose potential in PBS was within 30 mV of a SCE (similar to that reported for Ag pseudo-references in other background electrolyte solutions with like chloride-ion concentration [27]), and a stainless-steel needle as the auxiliary electrode (0.8 mm diameter). The working electrodes were platinum-iridium wires (90:10 ratio, Advent Research Materials Ltd., Oxford, UK) stress-relieved Teflon ® -insulated wire, 125-µm internal diameter.…”

Section: Methodsmentioning

confidence: 99%

“…Oxidizing this enzyme-generated HP (Equation (3)) requires a relatively high applied potential (0.4–0.7 V vs. SCE [27,28,29]) to provide good HP sensitivity. At these potentials, however, endogenous reducing agents, such as ascorbic acid (AA), which is present in high concentrations in most biological tissues and fluids, can give rise to false positive biosensor responses in vivo .…”

Section: Introductionmentioning

confidence: 99%

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Characterization of Biosensors Based on Recombinant Glutamate Oxidase: Comparison of Crosslinking Agents in Terms of Enzyme Loading and Efficiency Parameters

Ford

Quinn

O’Neill

2016

Sensors

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Amperometric l-glutamate (Glu) biosensors, based on both wild-type and a recombinant form of l-glutamate oxidase (GluOx), were designed and characterized in terms of enzyme-kinetic, sensitivity and stability parameters in attempts to fabricate a real-time Glu monitoring device suitable for future long-term detection of this amino acid in biological and other complex media. A comparison of the enzyme from these two sources showed that they were similar in terms of biosensor performance. Optimization of the loading of the polycationic stabilization agent, polyethyleneimine (PEI), was established before investigating a range of crosslinking agents under different conditions: glutaraldehyde (GA), polyethylene glycol (PEG), and polyethylene glycol diglycidyl ether (PEGDE). Whereas PEI-free biosensor designs lost most of their meager Glu sensitivity after one or two days, configurations with a 2:5 ratio of dip-evaporation applications of PEI(1%):GluOx(400 U/mL) displayed a 20-fold increase in their initial sensitivity, and a decay half-life extended to 10 days. All the crosslinkers studied had no effect on initial Glu sensitivity, but enhanced biosensor stability, provided the crosslinking procedure was carried out under well-defined conditions. The resulting biosensor design based on the recombinant enzyme deposited on a permselective layer of poly-(ortho-phenylenediamine), PoPD/PEI2/GluOx5/PEGDE, displayed good sensitivity (LOD < 0.2 μM), response time (t90% < 1 s) and stability over a 90-day period, making it an attractive candidate for future long-term monitoring of Glu concentration dynamics in complex media.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Characterization of Biosensors Based on Recombinant Glutamate Oxidase: Comparison of Crosslinking Agents in Terms of Enzyme Loading and Efficiency Parameters

Ford

Quinn

O’Neill

2016

Sensors

View full text Add to dashboard Cite

show abstract

“…[70,71] The dependence of the peak currents on the scan rate (Figure 6B) and the square root of the scan rate ( Figure 6C) were also studied. [72] Figure 7 shows the chronoamperograms and the calibration curves obtained for 0.0 to 16 mm glucose (in phosphate buffer solution (PBS), pH 7.4, E app = À0.10 V) by using our nonoptimized DPR-700/Fe 3 O 4 @pDA150/Gox configuration. The present analysis showed that the anodic and cathodic current peaks were linearly proportional to the v 1/2 value, which is expected for a diffusion-controlled process.…”

Section: Ir Spectroscopymentioning

confidence: 99%

“…The present analysis showed that the anodic and cathodic current peaks were linearly proportional to the v 1/2 value, which is expected for a diffusion-controlled process. [72] Figure 7 shows the chronoamperograms and the calibration curves obtained for 0.0 to 16 mm glucose (in phosphate buffer solution (PBS), pH 7.4, E app = À0.10 V) by using our nonoptimized DPR-700/Fe 3 O 4 @pDA150/Gox configuration. Data showed a linear dependence at low glucose concentrations and saturation at high concentrations, in agreement with the Michaelis-Menten kinetics.…”

Section: Ir Spectroscopymentioning

confidence: 99%

Quinone‐Rich Poly(dopamine) Magnetic Nanoparticles for Biosensor Applications

et al. 2014

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Novel core-shell quinone-rich poly(dopamine)-magnetic nanoparticles (MNPs) were prepared by using an in situ polymerization method. Catechol groups were oxidized to quinone by using a thermal treatment. MNPs were characterized by using X-ray diffraction, X-ray photoelectron spectroscopy, atomic force microscopy, magnetic force microscopy, UV/Vis, Fourier-transform infrared spectroscopy, and electrochemical techniques. The hybrid nanomaterial showed an average core diameter of 17 nm and a polymer-film thickness of 2 nm. The core-shell nanoparticles showed high reactivity and were used as solid supports for the covalent immobilization of glucose oxidase (Gox) through Schiff base formation and Michael addition. The amount of Gox immobilized onto the nanoparticle surface was almost twice that of the nonoxidized film. The resulting biofunctionalized MNPs were used to construct an amperometric biosensor for glucose. The enzyme biosensor has a sensitivity of 8.7 mA M(-1) cm(-2) , a low limit of detection (0.02 mM), and high stability for 45 days. Finally, the biosensor was used to determine glucose in blood samples and was checked against a commercial glucometer.

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“…This value is in the same order of magnitude with H 2 O 2 sensors in the literature. 54,55 Chronoamperometric data can also be used for estimation of the diffusion coefficient ( D) for an electroactive material using Cottrell's equation:…”

Section: Chronoamperometry Studies and Amperometric Detection Of H 2 Omentioning

confidence: 99%

Determination of hydrogen peroxide with an enzymeless amperometric sensor based on poly(vinylferrocene)-supported Ag nanoparticles

Çelebi¹,

Ozturk²,

Dumangöz³

et al. 2018

Turk J Chem

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An enzymeless electrochemical sensor for detection of low amounts of H 2 O 2 with the aid of Ag nanoparticles supported on conducting poly(vinylferrocene) (PVF) film was developed. Experimental results revealed that contribution of Ag nanoparticles led to remarkable improvement by means of reduction potential and reduction current. Influence of experimental parameters (i.e. polymeric film thickness, concentration of Ag precursor, immersion time in precursor solution, reduction time, and reduction potential) were investigated. The Ag/PVF-modified electrode system was characterized physically by scanning electron microscopy. The results revealed that the sensor developed was easyto-prepare, economic, selective, and sensitive, with a fast response time of 3 s. The linear concentration range of the sensor was 0.1-50 mM, with a sensitivity of 14.1 µ A mM −1 and a limit of detection of 0.94 µ M. Finally, interference effects of uric acid, ascorbic acid, dopamine, and glucose molecules were studied and no significant interference was observed at physiological levels.

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Improvement and characterization of surfactant-modified Prussian blue screen-printed carbon electrodes for selective H2O2 detection at low applied potentials

Cited by 48 publications

References 43 publications

Characterization of Biosensors Based on Recombinant Glutamate Oxidase: Comparison of Crosslinking Agents in Terms of Enzyme Loading and Efficiency Parameters

Characterization of Biosensors Based on Recombinant Glutamate Oxidase: Comparison of Crosslinking Agents in Terms of Enzyme Loading and Efficiency Parameters

Quinone‐Rich Poly(dopamine) Magnetic Nanoparticles for Biosensor Applications

Determination of hydrogen peroxide with an enzymeless amperometric sensor based on poly(vinylferrocene)-supported Ag nanoparticles

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