Improvementofsugarcane for borer resistance usingAgrobacteriummediated transformation ofcry1Acgene

Dessoky, Eldessoky S.; Ismail, Roba M.; Elarabi, Nagwa I.; Abdelhadi, Abdelhadi A.; Abdallah, Naglaa A.

doi:10.1080/21645698.2020.1809318

Cited by 30 publications

(10 citation statements)

References 45 publications

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“…In 1990, the first transgenic sugarcane was produced using the gun bombardment method (Bower & Birch, 1992). Arencibia et al (1998) later reported a novel sugarcane Agrobacterium-mediated transformation method which has been widely used in the field of sugarcane molecular breeding (Dessoky et al, 2020;Li et al, 2018). Augustine et al (2015) determined the high expression of UBI promoters compared with the CaMV 35S promoter using the Agrobacterium-mediated method in sugarcane.…”

Section: Discussionmentioning

confidence: 99%

Overexpression of sugarcane SoTUA gene enhances cold tolerance in transgenic sugarcane

et al. 2021

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Alpha (α)-tubulin (TUA) is a primary unit of cytoskeleton microtubules. In recent years, more frequent frosts have caused considerable yield loss in sugarcane (Saccharum spp. hybrid) production. Temperature is one of the most influential abiotic factors affecting the microtubule polymerization state in plants. In this research, we developed overexpressing SoTUA transgenic sugarcane lines from the cold-susceptible cultivar ROC22. The transgene was confirmed by polymerase chain reaction (PCR) and quantificational real-time polymerase chain reaction (qRT-PCR) analysis, while the relative expression of SoTUA protein was verified by Western blot. Compared with the nontransformed control plants (wild type [WT]), the transgenic lines showed higher relative protein expression than the WT at 25 ˚C, which indicates the overexpression of SoTUA protein. The contents of soluble protein and sugar and the activity of peroxidase (POD) were higher while the content of maleicdialdehyde was lower in the transgenic plants than the WT plants under the chilling treatment. Meanwhile, the expression levels of cold-defense related genes (P5CS, WRKY, Cu/Zn-SOD, and POD1) were higher in the transgenic lines than in the WT. These results suggest that the SoTUA protein plays an important role in protecting plants during chilling stress. This positive effect is not affected by transgenic plant generations.

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Section: Discussionmentioning

confidence: 99%

Overexpression of sugarcane SoTUA gene enhances cold tolerance in transgenic sugarcane

et al. 2021

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“…A. trigonus calli explants were immersed in the Agrobacterium suspension for 5 min. Thereafter, the explants were blotted on a sterilized filter paper, placed onto a co-cultivation media (MS medium supplemented with 0.5 mg/l 2, 4-D) and were incubated under dark conditions 52 . After co-cultivation for three days, the transformed calli were transferred to MS media including 0.5 mg/l NAA, 0.5 mg/l BAP, 500 mg/l cefotaxime and 1.5 mg/l bialaphos (Sigma-Aldrich, Japan) to select the transformed calli for shoot induction 53 .…”

Section: Methodsmentioning

confidence: 99%

Overexpression of chalcone isomerase A gene in Astragalus trigonus for stimulating apigenin

Elarabi

Abdelhadi

Sief-Eldein

et al. 2021

Sci Rep

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Apigenin is one of the most studied flavonoids and is widely distributed in the plant kingdom. Apigenin exerts important antioxidant, antibacterial, antifungal, antitumor activities, and anti-inflammatory effects in neurological or cardiovascular disease. Chalcone isomerase A (chiA) is an important enzyme of the flavonoid biosynthesis pathway. In order to enhance the apigenin production, the petunia chi A gene was transformed for Astragalus trigonus. Bialaphos survived plants were screened by PCR, dot blot hybridization and RT-PCR analysis. Also, jasmonic acid, salicylic acid, chitosan and yeast extract were tested to evaluate their capacity to work as elicitors for apigenin. Results showed that yeast extract was the best elicitor for induction of apigenin with an increase of 3.458 and 3.9 fold of the control for calli and cell suspension culture, respectively. Transformed cell suspension showed high apigenin content with a 20.17 fold increase compared to the control and 6.88 fold more than the yeast extract treatment. While, transformed T1 calli derived expressing chiA gene produced apigenin 4.2 fold more than the yeast extract treatment. It can be concluded that the highest accumulation of apigenin was obtained with chiA transgenic cell suspension system and it can be utilized to enhancement apigenin production in Astragalus trigonus.

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“…Gene gun-mediated transformation and AMT are among the most efficient and commonly utilized gene delivery methods for plant-related genetic transformation. These methods have been adopted for various crops, including soybeans (Li et al, 2017;Zhao et al, 2019;de Melo et al, 2020), sorghum (Che et al, 2018;Liu G. et al, 2019;de Melo et al, 2020;Sharma et al, 2020), maize (Char et al, 2017;Anand et al, 2018;Raji et al, 2018), sugarcane (Mayavan et al, 2015;Wu et al, 2015;Dessoky et al, 2021), wheat (Liang et al, 2018;Kumar et al, 2019;Zhang et al, 2019c), and rice (Endo et al, 2015;Ling et al, 2016;Feng et al, 2017).…”

Section: Conventional Plant Biomolecule Delivery Approaches and Their Limitationsmentioning

confidence: 99%

Advantage of Nanotechnology-Based Genome Editing System and Its Application in Crop Improvement

et al. 2021

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Agriculture is an important source of human food. However, current agricultural practices need modernizing and strengthening to fulfill the increasing food requirements of the growing worldwide population. Genome editing (GE) technology has been used to produce plants with improved yields and nutritional value as well as with higher resilience to herbicides, insects, and diseases. Several GE tools have been developed recently, including clustered regularly interspaced short palindromic repeats (CRISPR) with nucleases, a customizable and successful method. The main steps of the GE process involve introducing transgenes or CRISPR into plants via specific gene delivery systems. However, GE tools have certain limitations, including time-consuming and complicated protocols, potential tissue damage, DNA incorporation in the host genome, and low transformation efficiency. To overcome these issues, nanotechnology has emerged as a groundbreaking and modern technique. Nanoparticle-mediated gene delivery is superior to conventional biomolecular approaches because it enhances the transformation efficiency for both temporal (transient) and permanent (stable) genetic modifications in various plant species. However, with the discoveries of various advanced technologies, certain challenges in developing a short-term breeding strategy in plants remain. Thus, in this review, nanobased delivery systems and plant genetic engineering challenges are discussed in detail. Moreover, we have suggested an effective method to hasten crop improvement programs by combining current technologies, such as speed breeding and CRISPR/Cas, with nanotechnology. The overall aim of this review is to provide a detailed overview of nanotechnology-based CRISPR techniques for plant transformation and suggest applications for possible crop enhancement.

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Improvementofsugarcane for borer resistance usingAgrobacteriummediated transformation ofcry1Acgene

Cited by 30 publications

References 45 publications

Overexpression of sugarcane SoTUA gene enhances cold tolerance in transgenic sugarcane

Overexpression of sugarcane SoTUA gene enhances cold tolerance in transgenic sugarcane

Overexpression of chalcone isomerase A gene in Astragalus trigonus for stimulating apigenin

Advantage of Nanotechnology-Based Genome Editing System and Its Application in Crop Improvement

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