Improvement in bovine embryo production in vitro by glutathione-containing culture media

Luvoni, G.C.; Keskintepe, Levent; Brackett, Benjamin G.

doi:10.1002/(sici)1098-2795(199604)43:4<437::aid-mrd5>3.0.co;2-q

Cited by 139 publications

(90 citation statements)

References 54 publications

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“…Although we could not find any specific references for the mouflon in this regard, other studies (Park et al 1996;Luvoni et al 1996) have shown that the addition of SOD during IVF has a detrimental effect on the fertilization rates in porcine and bovine species. Park and colleagues (1996) have compared the effects of different antioxidants (catalase (CAT), SOD, mercaptoethanol (ME)) during IVM and IVF of porcine oocytes.…”

Section: Discussionmentioning

confidence: 99%

“…Superoxide dismutase was used at a concentration of 0.01, 0.1 or 1 mg mL −1 during both IVM and IVF; it did not influence nuclear maturation, but was found to inhibit penetration rates in a dose-dependent manner if added during IVF. The same result was obtained by Luvoni et al (1996). They studied the effect of adding 1500 or 3000 IU mL −1 of SOD during IVM, IVF and IVC on bovine oocytes and presumptive zygotes.…”

Section: Discussionmentioning

confidence: 99%

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Superoxide dismutase affects the viability of thawed European mouflon (Ovis g. musimon) semen and the heterologous fertilization using both IVF and intracytoplasmatic sperm injection

Berlinguer

Ledda

Rosati

et al. 2003

Reprod. Fertil. Dev.

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Abstract. This study evaluated the effects of superoxide dismutase (SOD) on viability and acrosome integrity of European mouflon spermatozoa after cryopreservation and on the fertilization rates of sheep oocytes after IVF or intracytoplasmatic sperm injection (ICSI). Frozen semen was thawed and washed with synthetic oviduct fluid supplemented with 0.6% bovine serum albumin. After centrifugation, the spermatozoa pellet was split into two culture systems: (i) without SOD; and (ii) in the presence of 1500 IU mL −1 SOD. Sperm viability and acrosome integrity were evaluated simultaneously, immediately after thawing and after 3, 6 and 9 h of culture (5% CO 2 , 39 • C, 90% humidity), by incubating sperm with propidium iodide and fluorescein isothiocyanate-labelled Pisum sativum agglutinin. At the same time, sperm were assessed for motility using a standard scoring system (independent operators' observation of sperm) that graded degree of motility (i.e. 1 = immotile to 10 = maximum motility, as observed at the moment of thawing). For IVF, frozen-thawed semen derived from the two culture systems was placed in culture together with in vitro-matured sheep oocytes. For ICSI, semen derived from the same culture systems as that for IVF was used, and incubated for 1 h under standard conditions. The results showed a marked difference (P < 0.01) between the percentages of live spermatozoa in medium with SOD and those obtained in medium alone, after 3, 6 and 9 h of culture. The percentages of intact acrosome spermatozoa were higher in medium with SOD after 6 h (P = 0.05) of culture. Spermatozoa motility decreased significantly in SOD containing medium at 3 and 6 h of culture compared with motility in control medium. Fertilization rates were significantly lower in medium with SOD than in medium alone, whereas in the ICSI system fertilization rates were significantly higher in the presence of SOD. The results indicate that the addition of SOD to the culture media enhances the viability rates and the acrosome integrity of cryopreserved mouflon spermatozoa.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Superoxide dismutase affects the viability of thawed European mouflon (Ovis g. musimon) semen and the heterologous fertilization using both IVF and intracytoplasmatic sperm injection

Berlinguer

Ledda

Rosati

et al. 2003

Reprod. Fertil. Dev.

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“…Under physiological conditions, generation of ROS occurs during various cellular metabolic reactions which is equilibrated by antioxidant defense systems of cells in order to neutralize the reactive intermediate [4,6]. In the in vivo condition, enzymatic and non-enzymatic antioxidants provide adequate protection for OS-induced pathological changes and maintain an optimal level of ROS, whereas in the in vitro setup, higher oxygen levels and lack of physiological defense mechanisms against ROS result in OS (Sajal [34,47]). Also, it has been shown that, OS can be induced during ART procedure by manipulation of gametes and embryos [50].…”

Section: Introductionmentioning

confidence: 99%

The effect of alpha lipoic acid on the developmental competence of mouse isolated preantral follicles

Talebi

Zavareh

Kashani

et al. 2012

J Assist Reprod Genet

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Purpose This study was designed to investigate the effect of alpha-lipoic acid (ALA) on reactive oxygen species (ROS) production, total antioxidant capacity (TAC) and developmental competence of cultured pre-antral follicles derived from mouse ovarian tissue. Methods Pre-antral follicles were isolated from immature mouse ovaries and were cultured in α-minimal essential medium supplemented with different concentrations (0, 50, 100, 250 and 500 uM) of ALA. Follicular growth, oocyte maturation and embryo development were evaluated. Separately, ROS and TAC were measured after 0, 24, 48, 72 and 96 h of culture with spectrofluorometery and ferric reducing/antioxidant power (FRAP) assay, respectively. Results In the presence of 100 uM ALA, developmental rates of follicles, oocytes and embryos were significantly higher than other groups (p<0.05). At 96 h after culture, a decrease in ROS and an increase in TAC were observed in ALA group compared to control group (p<0.05). Conclusion ALA (100 uM) improves the in vitro development of follicles. This effect may be mediated by decreasing ROS concentration and increasing follicular TAC level during the culture period.

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“…Esto podría deberse a que el medio empleado en la maduración, TCM 199, contiene una cantidad pequeña de glutatión (GSH) y de su precursor, la cisteína. La presencia de esta cantidad de GSH podría ser suficiente para proteger a los ovocitos del estrés oxidativo y la cisteína sería capaz de mantener un buen nivel de síntesis de GSH durante la maduración in vitro (Luvoni et al, 1996).…”

Section: Discussionunclassified

Efecto de la Suplementación del Medio de Maduración con Cisteamina y de Dos Medios de Cultivo (KSOMaa y SOF) en la Fecundación in vitro de Ovocitos Bovinos

Quintanilla

Huanca

Córdova

et al. 2015

Rev. investig. vet. Perú

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RESUMENSe evaluó el efecto de la cisteamina en el medio de maduración sobre la tasa de división posfecundación y el efecto de los medios de cultivo KSOMaa y SOF sobre la tasa de desarrollo embrionario in vitro hasta los 8 días posfecundación. Se utilizaron 680 ovocitos procedentes de ovarios de hembras bovinas distribuidos al azar en dos tratamientos: T 1 (n=321) medio de maduración TCM-199 y T 2 (n=359) medio de maduración TCM-199 adicionado con cisteamina 100 mM. Los ovocitos fueron fecundados a las 24 h con semen congelado de un solo toro y cultivados en medio KSOMaa por 18 h, evaluán-dose la tasa de división 72 h después. En la segunda fase, luego del primer cultivo, ambos grupos se subdividieron en dos grupos, donde el primero se cultivó con medio KSOMaa y el segundo con medio SOF hasta el día 8 posfecundación. Los resultados de la primera fase indican una tasa de división de 43.6% para el grupo control y 46.0% para el grupo suplementado con cisteamina, sin encontrar diferencia significativa. En la fase 2, se obtuvo una tasa de 18.0% de blastocistos para el tratamiento TCM-199 + KSOMaa y 20.9% para TCM-199 c/ cisteamina + KSOMaa (p<0.05), y 15.8% para TCM-199 + KSOMaa y SOF y 18.7% para TCM-199 c/ cisteamina + KSOMaa y SOF (p<0.05). Los resultados indican que la adición de cisteamina al medio de cultivo mejoró la tasa de desarrollo embrionario in vitro hasta el estadio de blastocisto en ovocitos madurados en medio TCM 199, no habiendo diferencias por el uso de los medios de cultivo.Palabras clave: fecundación in vitro, cisteamina, medio SOF, medio KSOM, bovino

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Improvement in bovine embryo production in vitro by glutathione-containing culture media

Cited by 139 publications

References 54 publications

Superoxide dismutase affects the viability of thawed European mouflon (Ovis g. musimon) semen and the heterologous fertilization using both IVF and intracytoplasmatic sperm injection

Superoxide dismutase affects the viability of thawed European mouflon (Ovis g. musimon) semen and the heterologous fertilization using both IVF and intracytoplasmatic sperm injection

The effect of alpha lipoic acid on the developmental competence of mouse isolated preantral follicles

Efecto de la Suplementación del Medio de Maduración con Cisteamina y de Dos Medios de Cultivo (KSOMaa y SOF) en la Fecundación in vitro de Ovocitos Bovinos

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