Improvement in infected wound healing in type 1 diabetic rat by the synergistic effect of photobiomodulation therapy and conditioned medium

Abstract

We investigated the effects of photobiomodulation therapy (PBMT) and conditioned medium (CM) of human bone marrow mesenchymal stem cells (hBM-MSC) individually and/or in combination on the stereological parameters and the expression of basic fibroblast growth factor (bFGF), hypoxia-inducible factor (HIF-1α), and stromal cell-derived factor-1α (SDF-1α) in a wound model infected with methicillin-resistant Staphylococcus aureus (MRSA) in diabetic rats. CM was provided by culturing hBM-MSCs. Type 1 diabetes mellitus (T1DM) was induced in 72 rats, divided into four groups, harboring 18 rats each: group 1 served as a control group, group 2 received PBMT, group 3 received CM, and group 4 received CM + PBMT. On days 4, 7, and 15, six animals from each group were euthanized and the skin samples were separated for stereology examination and gene expression analysis by real-time polymerase chain reaction. In the CM + PBMT, CM, and PBMT groups, significant decreases were induced in the number of neutrophils (1460 ± 93, 1854 ± 138, 1719 ± 248) and macrophages (539 ± 69, 804 ± 63, 912 ± 41), and significant increases in the number of fibroblasts (1073 ± 116, 836 ± 75, 912 ± 41) and angiogenesis (15 230 ± 516, 13 318 ± 1116, 14 041 ± 867), compared with those of the control group (2690 ± 371, 1139 ± 145, 566 ± 90, 12 585 ± 1219). Interestingly, the findings of the stereological examination in the CM + PBMT group were statistically more significant than those in the other groups. In the PBMT group, in most cases, the expression of bFGF, HIF-1α, and SDF-1α, on day 4 (27.7 ± 0.14, 28.8 ± 0.52, 27.5 ± 0.54) and day 7 (26.8 ± 1.4, 29.6 ± 1.4, 28.3 ± 1.2) were more significant than those in the control (day 4, 19.3 ± 0.42, 25.5 ± 0.08, 22.6 ± 0.04; day 7, 22.3 ± 0.22, 28.3 ± 0.59, 24.3 ± 0.19) and other treatment groups. The application of PBMT + CM induced anti-inflammatory and angiogenic activities, and hastened wound healing process in a T1 DM model of MRSA infected wound. K E Y W O R D S diabetes mellitus, diabetic foot ulcer, human bone marrow mesenchymal stem cell-conditioned medium, low-level laser therapy, methicillin-resistant staphylococcal aureus, phobiomodulation therapy, rat, real-time PCR, stereology J Cell Biochem. 2019;120:9906-9916. wileyonlinelibrary.com/journal/jcb 9906 |

Stereological and gene expression examinations on the combined effects of photobiomodulation and curcumin on wound healing in type one diabetic rats

“…T1DM rats. 25 Only PBM and PBM + curcumin increased fibroblast levels. administration on skin injury repair in an STZ-induced T1DM rat model.…”

“…Table lists the primers used to amplify cDNA. For each sample, we produced three replicates for each target gene …”

“…For each sample, we produced three replicates for each target gene. 25 F I G U R E 1 A schematic illustration of the wound and PBMtreated area. PBM, photobiomodulation…”

Photobiomodulation plus Adipose-derived Stem Cells Improve Healing of Ischemic Infected Wounds in Type 2 Diabetic Rats

“…The authors showed that photobiomodulation (890 nm, 80 Hz, 0.324 J/cm 2 ) meaningfully hastened the repair of injured skin at the maturation step and significantly decreased colony forming units (CFUs) of bacteria in a non-genetic model of DM2 in rats 26 .More than 100 recognized factors are involved in the insufficiency of wound healing in patients with DM2 27 . The use of a mixture of therapeutic agents and biological biostimulators could assist with treatment of non-healing wounds and show a synergistic effect to improve the success of healing in different wound models [28][29][30] .In the present study we focused on the effects of photobiomodulation and ADS, alone and combined, on the maturation step of an ischemic, delayed healing, infected wound model in DM2 rats. The combined application of photobiomodulation and ADS could hasten the healing process and provide a treatment for severe cases of DFUs in patients with DM2.…”

“…More than 100 recognized factors are involved in the insufficiency of wound healing in patients with DM2 27 . The use of a mixture of therapeutic agents and biological biostimulators could assist with treatment of non-healing wounds and show a synergistic effect to improve the success of healing in different wound models [28][29][30] .…”