Improvement in nitrogen fixation capacity could be part of the domestication process in soybean

Muñoz, Nacira; X, Qi; Li, Man-Wah; Xie, Min; Gao, Yongli; My, Cheung; Fl, Wong; Lam, Hon‐Ming

doi:10.1038/hdy.2016.27

Cited by 53 publications

(46 citation statements)

References 47 publications

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“…Moreover, the fast development of the metaxylem duct undoubtedly contributes to the transportation of substances, and the thickening of the cell walls of these structures improves the support function of the roots. Soybean roots contain bacteria in the nodule for nitrogen fixation, which is apart from conduction or support (Munoz et al, 2016). In our results, as compared to the wild soybeans, the cultivated soybeans essentially exhibited a decreasing trend in the protein expression levels in their root tips.…”

Section: Discussionsupporting

confidence: 61%

Anatomical Observation and Proteomics Analysis of Root Tips of Wild and Cultivated Soybeans

Xing¹,

Zhang²,

Ca³

et al. 2017

IJAB

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Wild and cultivated soybeans from different areas of China were used as materials for paraffin cross-sections of the juncture between the root elongation and maturation zones of wild and cultivated soybeans during the seedling stage. It was found that the casparian band of the endodermis and duct of metaxylem in the anatomical structure of the cultivated soybean developed faster than those of the wild soybean. Rapid development of these structures sped up transmission and enhanced support capacity. Meanwhile, two-dimensional electrophoresis was adopted to analyze the differences between the wild and cultivated soybean populations in terms of protein expression during the seedling stage. Our results showed that expression levels of 28 proteins from among the wild and cultivated soybean populations were significantly different from one another. In the cultivated population, the protein expression levels were up-regulated in four protein spots and down-regulated in 24 protein spots. Down-regulated proteins were associated with secondary metabolites and metabolism of antibiotics. Decrease in the expression these proteins may speed up nodule formation. These results showed that early growth and development of the cultivated soybean roots occurred at a significantly faster rate than those of the wild soybean. The increased rates of these processes accelerate root shape formation and improve root function. In conclusion, this study systematically explored the anatomical structure and differentially expressed proteins of roots between wild and cultivated soybean, and differentially expressed proteins identified here will be the valuable resource for studying soybean root differences resulting from domestication.

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Section: Discussionsupporting

confidence: 61%

Anatomical Observation and Proteomics Analysis of Root Tips of Wild and Cultivated Soybeans

Xing¹,

Zhang²,

Ca³

et al. 2017

IJAB

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“…Interestingly, the position of QNN17-2 identified on Gm17 overlapped with findings from another study, which strongly indicated that this QTL might be associated with nodule traits and NopB. A QTL localized on Gm17 was detected by using a RI population inoculated with USDA110, which was identified from total ureides commonly used as parameters to measure nitrogen fixation (Muñoz et al, 2016). We only detected one QTL localized on Gm10 called QNDW10-1, which was not found in previous research.…”

Section: Discussionsupporting

confidence: 60%

“…However, the positions of QNN17‐1 and QNN17‐2 were located close to each other. Moreover, a QTL was identified on chromosome 17 for total ureides using a recombinant inbred (RI) population inoculated with USDA110 (Muñoz et al, ) whose position overlapped with QNN17‐2 . In addition, only one QTL for NDW was identified after inoculation with the HH103Ω nopB mutant.…”

Section: Resultsmentioning

confidence: 99%

QTL mapping and gene mining to identify genes on soybean (Glycine max) associated with NopB of Sinorhizobium fredii HH103

Wang

Tian

et al. 2019

Plant Breeding

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Soybean is a special crop that can utilize N2 in the air via symbioses with Rhizobium spp. The formation of effective nodules is a complex process in which nodulation outer proteins (Nops) are determinants of establishment of a symbiotic relationship. We constructed a Sinorhizobium fredii HH103ΩnopB mutant. A nodulation test showed that the mutant had a negative effect on the Suinong14, ZYD00006, Dongnong594 and Charleston soybean lines. Recombinant inbred soybean lines were independently inoculated with the mutant and wild‐type strains, and five and four quantitative trait loci (QTLs) were identified by analysing the nodule number (NN) and nodule dry weight (NDW), respectively. We chose one QTL that overlapped with other studies and a novel QTL identified in our study and selected six candidate genes for further analysis. The qRT‐PCR analysis showed that only changes in Glyma.17G166200 expression depended on NopB. Further analysis showed that Glyma.17G166200 encoded a protein with a D‐glucose‐binding domain and a serine‐threonine/tyrosine protein kinase catalytic domain that was involved in the abscisic acid (ABA) pathway.

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“…Twenty-two soybean lines of different maturity groups (II-IX) and genetic backgrounds were selected for this study based on their potential N 2 fixation, nodulation, and higher yield in order to investigate the association of growth stages and N 2 fixation (Table 1). Additionally, three checks were included; the nonnodulating soybean cultivars Nitrasoy (Burton, Israel, & Bishop, 2006) and D68-099 (Hartwig, 1994) for estimating the amount of N derived from fixation as well as the supernodulating mutant SS2-2 (Youn et al, 2008;Youn et al, 2009) for evaluating the levels of N 2 fixation.…”

Section: Plant Materialsmentioning

confidence: 99%

<b>Di-nitrogen fixation at the early and late growth stages of soybean

Hamawaki

Kantartzi²

2018

Acta Sci. Agron.

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Soybean derives a significant portion of the required nitrogen (N) from the symbiosis with rhizobia bacteria. However, information on the available genetic variation for N 2 fixation capacity in different growth stages of soybean is limited. The objective of this study was to investigate the N 2 fixation capacity of 22 soybean lines compared with that of non-nodulating and supernodulating checks at the early and late growth stages and identify the most informative traits for selection. Two cycles of greenhouse experiments were carried out to estimate the percentage of N derived from the atmosphere (%Ndfa) as well as 10 different traits related to N 2 fixation. The results showed that %Ndfa was significantly different among the lines at the early and late growth stages. SPAD readings showed the highest correlation with the early N 2 fixation, whereas shoot dry weight with the late N 2 fixation. Early and late %Ndfa could be used to select superior lines for N 2 fixation and study the underlying physiological and molecular mechanism.Fixação de nitrogênio na fase inicial e final de desenvolvimento da soja RESUMO. Na soja uma parcela significativa do nitrogênio (N) requerido deriva de sua simbiose com a bactéria do gênero rhizóbio. No entanto, informações sobre variabilidade genética disponível para a capacidade de fixação de N 2 em diferentes estádios fenológicos é limitada. O objetivo deste estudo foi investigar a capacidade de fixação de N 2 durante a fase inicial e final de desenvolvimento de 22 genótipos de soja comparada a de genótipos não nodulantes e supernodulante utilizados como testemunhas, e também identificar os caracteres mais informativos para fins de seleção. Dois ciclos de experimentos foram conduzidos em casa de vegetação para estimar o percentual de N derivado da atmosfera (%Ndda), assim como outros 10 caracteres relacionados a fixação de N 2 . Os resultados demonstraram que o %Ndda foi significativamente diferente entre os genótipos nas duas avaliações. Leituras de fotossíntese com SPAD apresentaram a maior correlação com a avaliação inicial de fixação de N 2 , enquanto que a massa seca da parte aérea demonstrou a mais alta correlação com a avaliação de fixação de N 2 na fase final de desenvolvimento. Avaliações do %Ndda nas fases inicial e final de desenvolvimento da cultura podem ser utilizadas para seleção de genótipos superiores de soja para fixação de N 2 , assim como para o estudo dos mecanismos de controle nos âmbitos fisiológico e molecular. Palavras-chave: Glycine Max; %Ndda; técnica de diluição do nitrogênio-15; nodulação; simbiose.

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Improvement in nitrogen fixation capacity could be part of the domestication process in soybean

Cited by 53 publications

References 47 publications

Anatomical Observation and Proteomics Analysis of Root Tips of Wild and Cultivated Soybeans

Anatomical Observation and Proteomics Analysis of Root Tips of Wild and Cultivated Soybeans

QTL mapping and gene mining to identify genes on soybean (Glycine max) associated with NopB of Sinorhizobium fredii HH103

<b>Di-nitrogen fixation at the early and late growth stages of soybean

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