Improvement in physicochemical parameters of DPPC liposomes and increase in skin permeation of aciclovir and minoxidil by the addition of cationic polymers

Hasanovic, Amra; Hollick, Caroline; Fischinger, Kerstin; Valenta, Claudia

doi:10.1016/j.ejpb.2010.03.014

Cited by 68 publications

(29 citation statements)

References 26 publications

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“…These results show that except for two systems, polydispersity of the rest liposomes lies in the range of 0.15-0.20 at both temperatures and do not change much during 60 min of their contact with the PLC enzyme. Similar PDI index of DPPC liposomes, but of smaller size, was obtained by Hasanovic et al (2010). Obviously, from the polydispersity index the concentration of the liposomes cannot be determined.…”

Section: Particle Size Polydispersity and Zeta Potential Determinationmentioning

confidence: 49%

Zeta potential and surface charge of DPPC and DOPC liposomes in the presence of PLC enzyme

2016

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Zeta potential of liposomes is often measured in studies of their properties and/or applications. However, there are not many papers published in which zeta potential was determined during enzymatic reaction of a lipid. Therefore in this paper size, polydispersity index, and zeta potentials of 1,2-dipalmitoylsn-glycero-3-phosphatidylcholine (DPPC) and 1,2-dioleoyl-sn-glycero-3-phosphatidylcholine (DOPC) liposomes were investigated in 1 mM NaCl (pH 6.2) and phosphate buffer (pH 8.1) during 60 min of phospholipase C action at 20 and 37°C. The hydrocarbon chains saturation differ these two phospholipids what appears in some differences in the zeta potential changes during the hydrolysis reaction run. The polydispersity index of the liposomes indicates that they are relatively stable and monodisperse, except for DPPC in phosphate buffer where up to 30 % of the initial amount forms larger size moieties, possibly aggregates of the liposomes, enzyme and the hydrolysis products. However, in this buffer zeta potential of the liposomes practically does not change during PLC action. Also the changes of zeta potential of DOPC liposomes are minor, although their negative values are much smaller than those of DPPC at both temperatures. These small changes of the potential may be due to compression of the diffuse double layer by present phosphate buffer. However, distinct changes of the zeta potentials in the presence of PLC take place in NaCl solution. The observed changes can be explained by reorientation of the phospholipid polar heads and their different density on DPPC and DOPC surface of the liposomes. Although it appeared that the zeta potential is not a very sensitive parameter for tracking the hydrolysis reaction in phosphate buffer, generally zeta potential enables the characterization of such reactions through determination of electrokinetic properties of liposomes as well as the polydispersity and size distribution of the liposomes do.

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Section: Particle Size Polydispersity and Zeta Potential Determinationmentioning

confidence: 49%

Zeta potential and surface charge of DPPC and DOPC liposomes in the presence of PLC enzyme

2016

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“…This has been suggested to be as a result of the stronger electrostatic interaction between the positively charged liposomes and the skin lipids/skin surface, which further enhances permeation. This was demonstrated by Hasanovic et al (Hasanovic et al, 2010) in a study to show that the addition of cationic polymers such as chitosan to DPPC liposomes increased the skin permeation of the positively charged-coated liposomes in comparison to the uncoated liposomes.…”

Section: 0 Discussionmentioning

confidence: 72%

Doxorubicin liposomes as an investigative model to study the skin permeation of nanocarriers

Boakye

Patel

Singh

2015

International Journal of Pharmaceutics

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The objectives of this study were to develop an innovative investigative model using doxorubicin as a fluorophore to evaluate the skin permeation of nanocarriers and the impact of size and surface characteristics on their permeability. Different doxorubicin-loaded liposomes with mean particle size <130nm and different surface chemistry were prepared by ammonium acetate gradient method using DPPC, DOPE, Cholesterol, DSPE-PEG 2000 and 1,1-Di-((Z)-octadec-9-en-1-yl)pyrrolidin-1-ium chloride (CY5)/DOTAP/1,2-dioleoyl-sn-glycero-3-phosphate (DOPA) as the charge modifier. There was minimal release of doxorubicin from the liposomes up to 8 h; indicating that fluorescence observed within the skin layers was due to the intact liposomes. Liposomes with particle sizes >600nm were restricted within the stratum corneum. DOTAP (p<0.01) and CY5 (p<0.05) liposomes demonstrated significant permeation into the skin than DOPA and PEG liposomes. Tape stripping significantly (p<0.01) enhanced the skin permeation of doxorubicin liposomes but TAT-decorated doxorubicin liposomes permeated better (p<0.005). Blockage of the hair follicles resulted in significant reduction in the extent and intensity of fluorescence observed within the skin layers. Overall, doxorubicin liposomes proved to be an ideal fluorophore-based model. The hair follicles were the major route utilized by the liposomes to permeate skin. Surface charge and particle size played vital roles in the extent of permeation.

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“…The fluorescent marker (Rhodamine 123) loaded formulations were applied topically to shaved dorsal portion of albino nude rat, and 1 and 2 h after application rats were euthanized. The representative sections were obtained from rats and skin was cut into small pieces, fixed in 10% formalin, embedded in paraffin and sections were then stained as reported elsewhere [25], and were observed under fluorescence microscope.…”

Section: Fluorescence Microscopymentioning

confidence: 99%

Elastic liposome-mediated transdermal immunization enhanced the immunogenicity of P. falciparum surface antigen, MSP-119

Tyagi

Garg

Jadon

et al. 2015

Vaccine

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Improvement in physicochemical parameters of DPPC liposomes and increase in skin permeation of aciclovir and minoxidil by the addition of cationic polymers

Cited by 68 publications

References 26 publications

Zeta potential and surface charge of DPPC and DOPC liposomes in the presence of PLC enzyme

Zeta potential and surface charge of DPPC and DOPC liposomes in the presence of PLC enzyme

Doxorubicin liposomes as an investigative model to study the skin permeation of nanocarriers

Elastic liposome-mediated transdermal immunization enhanced the immunogenicity of P. falciparum surface antigen, MSP-119

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