Improvement in the staining and in the visualization of the argyrophilic proteins of the nucleolar organizer region at the optical level

Ploton, Dominique; Ménager, M.; Jeannesson, Pierre; Himber, G; Pigeon, Françoise Carré; Adnet, J. J.

doi:10.1007/bf01676192

Cited by 951 publications

(483 citation statements)

References 17 publications

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“…it has been demonstrated that the bcl-2/bax mRNA expression ratio represents a prognostic marker in low-grade urinary (Ploton et al 1986). AgNOR staining.…”

mentioning

confidence: 99%

Bax expression has prognostic significance that is enhanced when combined with AgNOR counts in glottic carcinomas

Xie

Clausen²,

Angelis³

et al. 1998

Br J Cancer

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Recently. a family of genes whose encoded proteins share amino acid sequence homology with Bcl-2 have been identified. This family of proteins include Bcl-2. Bcl-x and Mcl-l. which act as blockers of apoptosis. whereas others such as Bax and Bak appear to promote apoptosis (Oltvai. 1993: Boise et al. 1995. The biological mechanisms by which the Bcl-2 gene family regulate apoptosis remain uncertain. The Bax protein seems. however. to act as a central regulator within this multigene family. Several studies have demonstrated that increased Bax expression is associated with increased radio-and chemosensitivity to induction of apoptosis (Bargou et al. 1996: Chresta et al. 1996: Kitada et al. 1996: Sakakura et al. 1996: Stoetzer et al. 1996: Thomas et al. 1996: Wagener et al. 1996. Information regarding the prognostic significance of Bax in human tumours is scarce. It has, however, been shown that reduced Bax expression correlates with tumour progression and shortened sunrival in breast adenocarcinomas (Krajewski et al. 1995).Moreover. it has been demonstrated that the bcl-2/bax mRNA expression ratio represents a prognostic marker in low-grade urinary

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“…it has been demonstrated that the bcl-2/bax mRNA expression ratio represents a prognostic marker in low-grade urinary (Ploton et al 1986). AgNOR staining.…”

mentioning

confidence: 99%

Bax expression has prognostic significance that is enhanced when combined with AgNOR counts in glottic carcinomas

Xie

Clausen²,

Angelis³

et al. 1998

Br J Cancer

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“…NOR argyrophilia is related to acidic proteins associated with rRNA transcription sites and known to play a key role in the control of rRNA transcription and processing [9]. Nucleolin, protein B23, RNA polymerase I represent the main argyrophilic NORs proteins observed in cell nucleoli [7,[9][10][11][12][13] At the light microscopy level, AgNOR staining methods identify both the fibrillar centers and the associated dense fibrillar component as black dots [6].…”

Section: Discussionmentioning

confidence: 99%

“…Cell smears, bone and kidney sections were incubated for 55min at RT in the dark, in a staining solution prepared by combining silver nitrate (2 volumes of a 50% aqueous solution) with formic acid (1 volume of a 1% solution containing 2% gelatin) [6]. After staining, slides were thoroughly washed in distilled water (DW) and transferred for 10min to a 5% aqueous sodium thiosulfate solution prepared extemporaneously.…”

Section: Agnor Stainingmentioning

confidence: 99%

Osteopontin is an argentophilic protein in the bone matrix and in cells of kidney convoluted tubules

et al. 2007

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Nucleolar Organising Regions (NOR) are part of the nucleolus, containing argyrophilic proteins (nucleoclin/C23, nucleophosmin/B23). They are identified by silver staining at low pH. The method also reveals osteocyte canaliculi and cement lines and granules in the cytoplasm of kidney cells in locations that mimic osteopontin distribution. Human bone and kidney sections, benign and lymphomatous pleural effusions were processed for silver staining to identify AgNOR. Sections were processed in parallel for immunohistochemistry with an antibody direct against osteopontin. In pleural effusions, AgNORs were found increased in the nuclei of lymphoma cells. In bone, Ag staining identified AgNOR in cell nuclei, as well as in osteocyte canaliculi, cement and resting lines. In the distal convoluted tubules of the kidney, silver deposits were also observed in cytoplasmic granules on the apical side of the cells. Immunolocalization of osteopontin closely matched with all these locations in bone and kidney. NOR proteins and osteopontin are proteins containing aspartic acid rich repeats that can bind Ag.Staining protocols using silver nitrate at low pH can identify these proteins on histological sections. AgNOR is a useful histochemical method to identify osteopontin in bone sections. Short running title:Osteopontin and siver staining

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“…From the different silver staining procedures described (2,3,18), w e selected the procedure of Ploton et al (18). Briefly, washed cells grown on coverslips were fixed with paraformaldehyde (2% in PBS) for 20 min at 4°C then in 70% ethanol for an additional 20 min at 4°C.…”

Section: Silver Staining Of the Nucleolar Organizer Regionsmentioning

confidence: 99%

“…Each nucleolus consists of a fibrillar center, a dense fibrillary component, and a granular component (8,10,11,14,15,(20)(21)(22)(23). The NORs can be selectively visualized on both cytological preparations and paraffin sections by the silver-staining technique termed Ag-NOR (18). The relationship of Ag-NOR morphology to cellular proliferation and grade of malignancy is the subject of intense investigation.…”

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confidence: 99%

Combined light and fluorescent microscopical imaging of nucleolar organizer regions and cellular rRNA as detected by fluorescent in situ hybridization

Pajor

Honeyman

1995

Cytometry

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A method for combined light and fluorescent microscopic imaging of nucleolar organizer regions and cellular rRNA is described. Nucleolar organizer regions were detected by silver staining (Ag-NOR), and rRNA was detected by fluorescent in situ hybridization (FISH). MG-63 human fibrosarcoma cells were silver stained prior to in situ hybridization. To quantitate Ag-NOR within individual cells, brightfield images were digitized, and the total Ag-NOR arednucleus was determined. Fluorescent images were digitized, and the total cellular fluorescence was calculated after correction for nonuniformity of illumination. By using this method, it was shown that the Ag-NOR procedure did not signiticantly affect the fluorescence intensity related to FISH. Furthermore, the hybridization procedure did not interfere with quantitation of Ag-NOR. With this method, both Ag-NOR and rRNA product can be quantitated within the same cell. Because the relationship of rRNA content to cell proliferation is well established, correlation to quantitative Ag-NOR parameters within individual cells will contribute to the better definition of the relationship of quantitative Ag-NOR indices with cellular proliferation. 0 1995 Wiley-Liss, Inc.

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Improvement in the staining and in the visualization of the argyrophilic proteins of the nucleolar organizer region at the optical level

Cited by 951 publications

References 17 publications

Bax expression has prognostic significance that is enhanced when combined with AgNOR counts in glottic carcinomas

Bax expression has prognostic significance that is enhanced when combined with AgNOR counts in glottic carcinomas

Osteopontin is an argentophilic protein in the bone matrix and in cells of kidney convoluted tubules

Combined light and fluorescent microscopical imaging of nucleolar organizer regions and cellular rRNA as detected by fluorescent in situ hybridization

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