Improvement of Anaerobic Operation and Isolation Culture on Hydrogen-Producing and Fermentative Bacteria

Li, Yong Feng; Yang, Jian; Pan, Xin; Zhang, Jing Wei; Han, Wei

doi:10.4028/www.scientific.net/amr.183-185.895

Cited by 6 publications

(8 citation statements)

References 6 publications

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“…After sterilization under conditions of high pressure at 115℃ for 30 min, the isolated fermentation bacteria were cultured at 120r/min, and the culture temperature was 37℃. An improved Hungate rolling tubes technique was utilized for preparing the aseptic water, culture media, isolating culture and bacteria operation [7] .…”

Section: Methodsmentioning

confidence: 99%

Comparative Analysis and Improvement of C/N on Hydrogen Production with the Bacterium Strain B49

Duan

Liu

et al. 2011

AMR

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Anaerobic process of biohydrogen production was developed recently. The representative strain of hydrogen production bacteria, namely fermentative hydrogen producing bacterial strain B49，was isolated from the activated sludge which was cultured artificially by our lab. In this study, there were some different C/N ratios employed. Experiment results indicated that the concentration of carbon and nitrogen had a great effect upon hydrogen production of B49, when C/N=2.5, glucose got the highest degraded efficiency, and at a C/N ratio of 3.3, the bio-gas and hydrogen production peaked with 159mL and 37.5mL, respectively, which considered that the optimum C/N ratio on the growth and hydrogen production was 3:1. Furthermore, the mainly terminal liquid products of B49 were ethanol and acetic acid. With the increase of the total organic nitrogen, final pH value decreased gradually.

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Section: Methodsmentioning

confidence: 99%

Comparative Analysis and Improvement of C/N on Hydrogen Production with the Bacterium Strain B49

Duan

Liu

et al. 2011

AMR

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“…The time of culture is 20 days，evaluated the amount of bacterium by MPN method. HPB-LR medium and LM-1 medium were adopted in the experiments [15,17].…”

Section: Methodsmentioning

confidence: 99%

“…Nowadays, scientists only got some species that come from Clostridium genus and Enterobacter genus. Their genetic background is narrow and could not be used in practice [3,17]. For widening genetic basis of the bacterium and discovering the new species, we isolated some high efficient biohydrogen production anaerobic bacterium and made phylogenetic analysis.…”

Section: Introductionmentioning

confidence: 99%

Characterization and Phylogenetics of a New Species of High Efficient Biohydrogen Production Anaerobic Bacterium

Liu

Jiao

et al. 2010

AMR

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Anaerobic process of biohydrogen production was developed recently. The isolation and identification of high efficient biohydrogen production anaerobic bacteria is the important foundation of fermented biohydrogen production process by anaerobic digesting organic wastewater. Considering with the physiological and biochemical traits, morphological characteristics and 16SrDNA sequence, the isolate Rennanqilyf12 is a new species and possibly member of a new genus. The temporary nomenclature of the genus is Biohydrogenbacterium and the temporary nomenclature of the specie is Biohydrogenbacterium nanqiyongfengii sp.nov.

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“…In addition, the use of organic waste reduces waste disposal problems [ 5 , 6 ]. In addition to H 2 production, anaerobic fermentation also produces a significant amount of alcohols (such as ethanol) [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Biohydrogen and Bioethanol Production from Anaerobic Fermentation with Immobilized Sludge

Han

Wang²,

Chen³

et al. 2011

BioMed Research International

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The effects of organic loading rates (OLRs) on fermentative productions of hydrogen and ethanol were investigated in a continuous stirred tank reactor (CSTR) with attached sludge using molasses as substrate. The CSTR reactor with attached sludge was operated under different OLRs, ranging from 8 to 24 kg/m3·d. The H2 and ethanol production rate essentially increased with increasing OLR. The highest H2 production rate (10.74 mmol/h·L) and ethanol production rate (11.72 mmol/h·L) were obtained both operating at OLR = 24 kg/m3·d. Linear regression results show that ethanol production rate (y) and H2 production rate (x) were proportionately correlated and can be expressed as y = 1.5365x − 5.054 (r2 = 0.9751). The best energy generation rate was 19.08 kJ/h·L, which occurred at OLR = 24 kg/m3·d. In addition, the hydrogen yield was affected by the presence of ethanol and acetic acid in the liquid phase, and the maximum hydrogen production rate occurred while the ratio of ethanol to acetic acid was close to 1.

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Improvement of Anaerobic Operation and Isolation Culture on Hydrogen-Producing and Fermentative Bacteria

Cited by 6 publications

References 6 publications

Comparative Analysis and Improvement of C/N on Hydrogen Production with the Bacterium Strain B49

Comparative Analysis and Improvement of C/N on Hydrogen Production with the Bacterium Strain B49

Characterization and Phylogenetics of a New Species of High Efficient Biohydrogen Production Anaerobic Bacterium

Simultaneous Biohydrogen and Bioethanol Production from Anaerobic Fermentation with Immobilized Sludge

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