Improvement of Artificial Medium for Passionfruit Pollen Germination

Kozai, Naoko; Kataoka, Isao; Chusri, O.; Yonemoto, Yoshimi; Ogata, T.

doi:10.17660/actahortic.2008.787.15

Cited by 2 publications

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“…Culture medium containing a gelling agent (agar or gelatin) has been used successfully in pollen germination in many species (JAYAPRAKASH and SARLA, 2001;KAKANI et al, 2002;KOZAI et al, 2008). According to Stanley and Linskens (1974), the gelling agent facilitates the incorporation of sucrose or other stimulants of germination and maintains constant relative humidity and suitable aerobic conditions for adequate pollen grain germination.…”

Section: Discussionmentioning

confidence: 99%

In vitro pollen germination and pollen viability in passion fruit (Passiflora spp.)

et al. 2013

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-The use of Passiflora species for ornamental purposes has been recently developed, but little is known about pollen viability and the potential for crossing different species. The objective of this study was to evaluate the pollen viability of six Passiflora species collected from different physiological stages of development through in vitro germination and histochemical analysis using dyes. The pollen was collected in three stages (pre-anthesis, anthesis and post-anthesis). Three compositions of culture medium were used to evaluate the in vitro germination, and two dyes (2,3,5-triphenyltetrazolium chloride, or TTC, and Lugol's solution) were used for the histochemical analysis. The culture medium containing 0.03% Ca(NO 3 ) 4H 2 O, 0.02% of Mg(SO 4 ).7H 2 O, 0.01% of KNO 3 , 0,01% of H 3 BO 3 , 15% sucrose, and 0.8% agar, pH 7.0, showed a higher percentage of pollen grains germinated. Anthesis is the best time to collect pollen because it promotes high viability and germination. The Lugol's solution and TTC dye overestimated the viability of pollen, as all accessions showed high viability indices when compared with the results obtained in vitro. Index terms: passion fruit, pollen germination, histochemical analysis, wild passion fruit. GERMINAÇÃO DE PÓLEN IN VITRO E VIABILIDADE POLÍNICA EM MARACUJAZEIRO (Passiflora spp.)RESUMO -O uso de Passifloras como plantas ornamentais é recente, e por isso pouco se sabe sobre a possibilidade de cruzamento entre diferentes espécies, bem como sobre a viabilidade polínica. O objetivo do trabalho foi avaliar a viabilidade do pólen de seis espécies de maracujazeiro coletados em diferentes estádios de desenvolvimento fisiológico por meio da germinação in vitro, bem como via análise histoquímica, por meio do uso de corantes. O pólen foi coletado em três estádios (pré-antese, antese e pós-antese). Foram comparadas três composições de meios de cultura para avaliar a germinação in vitro, e para a análise histoquímica utilizou-se de dois corantes o 2,3,5-cloreto de trifeniltetrazólio (TTC) e o lugol. O meio de cultura contendo 0,03% de Ca(NO3).4H2O, 0,02% de Mg(SO4).7H2O, 0,01% de KNO3, 0,01% de H3BO3, 15% de sacarose, solidificado com 0,8% de ágar e pH ajustado para 7,0, proporcionou maior percentual de grãos de pólen germinados. O melhor momento de coleta do pólen com relação à melhor viabilidade e à germinação é na antese. O corante TTC e o lugol superestimaram a viabilidade de pólen, já que todos os acessos apresentaram índice de viabilidade alto, quando comparados aos resultados obtidos in vitro. Termos para Indexação: Maracujazeiro, germinação de pólen, análise histoquímica, espécies silvestres.

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Section: Discussionmentioning

confidence: 99%

In vitro pollen germination and pollen viability in passion fruit (Passiflora spp.)

et al. 2013

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“…Pollen Germination and Pollen Tube Growth. Culture medium containing a gelling agent (agar or gelatin) has been used successfully in many species (Jayaprakash & Sarla, 2001;Kakani et al, 2002;Kozai et al, 2008). In this study, agar was used instead of gelatin due to the report of Stanley & Liskens (1974) that there are several advantages of using agar germination tests such as the ease of taking carbohydrate, creating stable relative humidity and providing aerobic conditions.…”

Section: -8)mentioning

confidence: 99%

Comparative Pollen Viability and Pollen Tube Growth of two Endemic Philippine Etlingera (Zingiberaceae, Alpinioideae)

2017

Philippine Journal of Systematic Biology

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The pollen viability and pollen tube growth of Etlingera dalican and Etlingera philippinensis (Zingiberaceae) were examined from fresh samples under the light microscope (LM) and scanning electron microscope (SEM). Pollen measurements were 68-75μm (E. dalican) and 60-65μm (E. philippinensis), having spheroidal shape for hydrated pollen and irregularly-shape for dry pollen for both species. E. dalican pollen has greenish-yellow color while that of E. philippinensis is greenish. Both species had inaperturate pollen but differ in their ornamentation which is gemmate in E. dalican while psilate in E. philippinensis. E. dalican has 88.56% pollen viability while E. philippinensis has only 40.69%. The rate of pollen tube growth was faster (17.75 μm per day) in E. dalican than E. philippinensis (8.17 μm per day). The possible pollinators observed for the two species were butterflies of the genus Catopsilia, ants and flies. Additional information on the inflorescence and flower description of the two species are herein presented.

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Improvement of Artificial Medium for Passionfruit Pollen Germination

Cited by 2 publications

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In vitro pollen germination and pollen viability in passion fruit (Passiflora spp.)

In vitro pollen germination and pollen viability in passion fruit (Passiflora spp.)

Comparative Pollen Viability and Pollen Tube Growth of two Endemic Philippine Etlingera (Zingiberaceae, Alpinioideae)

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