Due to the short development time of cultural and tourism towns in China, local governments and investors lack experience in building cultural and tourism towns and do not pay enough attention to the positioning of towns. Alternatively, this issue results in chaos in domestic cultural and tourism towns and even a large number of empty towns in some provinces. Therefore, how to accurately locate cultural tourism towns is a problem that must be studied in depth at present. This paper uses the regional economic theory to collect the influencing factors of cultural tourism town positioning. Based on the BP neural network and the improved genetic algorithm, a genetic neural network model is constructed to train and predict the samples of cultural tourism towns. Taking a small town in the East as a case, the data were collected and analyzed. Established on the prediction outcomes of the genetic neural network, the best location of a small town was selected according to the actual situation of the region. In terms of accuracy and training time, our experimental evaluation confirmed that the neural network enhanced by genetic algorithms outperforms the conventional BP neural network. Furthermore, we observed that besides the classification capabilities of the BP neural network-based model, the classical BP neural network improved by the genetic algorithm also exhibits great macrosearch capabilities and good global optimization performance.