Improvement of bio-cementation at low temperature based on Bacillus megaterium

Sun, Xiaohao; Miao, Linchang; Wu, Linyu; Chen, Runfa

doi:10.1007/s00253-019-09986-7

Cited by 50 publications

(30 citation statements)

References 34 publications

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“…Related research demonstrated that the mass of calcium carbonate in the cemented sample at 50°C is three times that of 25°C, and its compressive strength is only 60% of that at 25°C [ 151 ]. Sun et al [ 152 ] domesticated the microorganisms and increased the concentration of urea in a low-temperature environment to make them grow and multiply faster and produce more precipitation and a better curing effect in the low-temperature sand fixation test. Additionally, the temperature affects the crystal size of calcium carbonate precipitation, and the optimal temperature for curing sandy soil is 20°C~30°C [ 153 ].…”

Section: Mineralization Influencing Factorsmentioning

confidence: 99%

Research status and development of microbial induced calcium carbonate mineralization technology

et al. 2022

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In nature, biomineralization is a common phenomenon, which can be further divided into authigenic and artificially induced mineralization. In recent years, artificially induced mineralization technology has been gradually extended to major engineering fields. Therefore, by elaborating the reaction mechanism and bacteria of mineralization process, and summarized various molecular dynamics equations involved in the mineralization process, including microbial and nutrient transport equations, microbial adsorption equations, growth equations, urea hydrolysis equations, and precipitation equations. Because of the environmental adaptation stage of microorganisms in sandy soil, their reaction rate in sandy soil environment is slower than that in solution environment, the influencing factors are more different, in general, including substrate concentration, temperature, pH, particle size and grouting method. Based on the characteristics of microbial mineralization such as strong cementation ability, fast, efficient, and easy to control, there are good prospects for application in sandy soil curing, building improvement, heavy metal fixation, oil reservoir dissection, and CO2 capture. Finally, it is discussed and summarized the problems and future development directions on the road of commercialization of microbial induced calcium carbonate precipitation technology from laboratory to field application.

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Section: Mineralization Influencing Factorsmentioning

confidence: 99%

Research status and development of microbial induced calcium carbonate mineralization technology

et al. 2022

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“…Durability and mechanical test Existing structure Pseudomonas bacteria [36][37][38][39] Compression strength [40][41][42] Self-healing [16,[43][44][45]] Bacillus subtilis [2,4,34] Tension strength [5,[46][47][48] Resettlement of original structure [1,16,49, 50] Megaterium bacteria [5,51,52] Flexural strength [3], [28,53,54] Cereus and sphaericus bacteria [55][56][57] Durability characteristics [12,19,[58][59][60] [71], a split tensile strength test is performed at the ages of seven days, twentyeight days, fty-six days, ninety days, one hundred twenty days, one hundred eighty days, two hundred seventy days, and three hundred sixty-ve days using a compressive testing machine with a capacity of two thousand kilonewtons. e loading part and the surface of the cylinder specimen are separated by a wooden strip so that there is no direct impact from the loading on the cylinder [55,72,73].…”

Section: Materials Usedmentioning

confidence: 99%

Performance Evaluation of Bio Concrete by Cluster and Regression Analysis for Environment Protection

Shukla

Gupta

Singh

et al. 2022

Computational Intelligence and Neuroscience

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The focus of this research is to isolating and identifying bacteria that produce calcite precipitate, as well as determining whether or not these bacteria are suitable for incorporation into concrete in order to enhance the material’s strength and make the environment protection better. In order to survive the high “potential of hydrogen” of concrete, microbes that are going to be added to concrete need to be able to withstand alkali, and they also need to be able to develop endospores so that they can survive the mechanical forces that are going to be put on the concrete while it is being mixed. In order to precipitate CaCO3 in the form of calcite, they need to have a strong urease activity. Both Bacillus sphaericus and the Streptococcus aureus bacterial strains were evaluated for their ability to precipitate calcium carbonate (CaCO3). These strains were obtained from the Department of Biotechnology at GLA University in Mathura. This research aims to solve the issue of augmenting the tension and compression strengths of concrete by investigating possible solutions for environmentally friendly concrete. The sterile cultures of the microorganisms were mixed with water, which was one of the components of the concrete mixture, along with the nutrients in the appropriate proportions. After that, the blocks were molded, and then pond-cured for 7, 28, 56, 90, 120, 180, 270, and 365 days, respectively, before being evaluated for compressibility and tensile strength. An investigation into the effect that bacteria have on compression strength was carried out, and the outcomes of the tests showed that bacterial concrete specimens exhibited an increase in mechanical strength. When compared to regular concrete, the results showed a maximum increase of 16 percent in compressive strength and a maximum increase of 12 percent in split tensile strength. This study also found that both bacterial concrete containing 106, 107, and 108 cfu/ml concentrations made from Bacillus sphaericus and Streptococcus aureus bacteria gave better results than normal concrete. Both cluster analysis (CA) and regression analysis (RA) were utilized in this research project in order to measure and analyze mechanical strength.

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“…More information about these bacteria can be found in our previous paper [45]. Bacillus megaterium was used as a control in this study, since they outperformed Sporosarcina pasteurii at temperatures around 15˚C [24,46].…”

Section: Application Of Bacteria Towards Precipitationmentioning

confidence: 99%

“…Microbial metabolic activities associated with MICP include ureolysis, denitrification, ammonification, sulphate reduction, and methane oxidation [4]. Different microorganisms have been found capable of MICP and the most studied urease bacteria are Sporosarcina pasteurii [6,[16][17][18][19][20][21] and Bacillus [22][23][24][25]. A recent review paper compared the performances of those microorganism towards MICP [26].…”

Section: Introductionmentioning

confidence: 99%

Microbially induced calcite precipitation performance of multiple landfill indigenous bacteria compared to a commercially available bacteria in porous media

et al. 2021

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Microbially Induced Carbonate Precipitation (MICP) is currently viewed as one of the potential prominent processes for field applications towards the prevention of soil erosion, healing cracks in bricks, and groundwater contamination. Typically, the bacteria involved in MICP manipulate their environment leading to calcite precipitation with an enzyme such as urease, causing calcite crystals to form on the surface of grains forming cementation bonds between particles that help in reducing soil permeability and increase overall compressive strength. In this paper, the main focus is to study the MICP performance of three indigenous landfill bacteria against a well-known commercially bought MICP bacteria (Bacillus megaterium) using sand columns. In order to check the viability of the method for potential field conditions, the tests were carried out at slightly less favourable environmental conditions, i.e., at temperatures between 15-17°C and without the addition of urease enzymes. Furthermore, the sand was loose without any compaction to imitate real ground conditions. The results showed that the indigenous bacteria yielded similar permeability reduction (4.79 E-05 to 5.65 E-05) and calcium carbonate formation (14.4–14.7%) to the control bacteria (Bacillus megaterium), which had permeability reduction of 4.56 E-5 and CaCO3 of 13.6%. Also, reasonably good unconfined compressive strengths (160–258 kPa) were noted for the indigenous bacteria samples (160 kPa). SEM and XRD showed the variation of biocrystals formation mainly detected as Calcite and Vaterite. Overall, all of the indigenous bacteria performed slightly better than the control bacteria in strength, permeability, and CaCO3 precipitation. In retrospect, this study provides clear evidence that the indigenous bacteria in such environments can provide similar calcite precipitation potential as well-documented bacteria from cell culture banks. Hence, the idea of MICP field application through biostimulation of indigenous bacteria rather than bioaugmentation can become a reality in the near future.

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Improvement of bio-cementation at low temperature based on Bacillus megaterium

Cited by 50 publications

References 34 publications

Research status and development of microbial induced calcium carbonate mineralization technology

Research status and development of microbial induced calcium carbonate mineralization technology

Performance Evaluation of Bio Concrete by Cluster and Regression Analysis for Environment Protection

Microbially induced calcite precipitation performance of multiple landfill indigenous bacteria compared to a commercially available bacteria in porous media

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