Improvement of cardiac function and remodeling by transplanting adipose tissue-derived stromal cells into a mouse model of acute myocardial infarction

Yu, Long; Kim, Moo Hyun; Park, Tae Ho; Soo, Kwang; Quan, Mei Lian; Rho, Mi Sook; Seo, Su Yeoung; Jung, Jin Sup

doi:10.1016/j.ijcard.2008.10.024

Cited by 40 publications

(25 citation statements)

References 20 publications

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“…Repairing a damaged heart would likely require regenerated myocardium, new blood vessels, nerve innervation, and electricalmechanical coupling of the restored tissue (Beeres et al 2008). Recent experimental studies have shown that adipose tissue-derived stem cells (ADSCs), a source of mesenchymal stem cells (MSCs), are self-renewing and capable of regenerating infracted myocardium by inducing myogenesis and angiogenesis (Hwangbo et al 2010;Lin et al 2010;Yu et al 2010). ADSCs and MSCs exhibit a highly similar gene expression profile (Noel et al 2008), and the stem cells isolated from adipose tissues share the same morphology, phenotype and in vitro differentiation ability with bone marrow.…”

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confidence: 99%

Transplantation of Adipose Tissue-Derived Stem Cells Overexpressing Heme Oxygenase-1 Improves Functions and Remodeling of Infarcted Myocardium in Rabbits

Yang

Liu

et al. 2012

Tohoku J. Exp. Med.

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Adipose tissue-derived stem cells (ADSCs) are a promising source of autologous stem cells that are used for regeneration and repair of infracted heart. However, the efficiency of their transplantation is under debate. One of the possible reasons for marginal improvement in ADSCs transplantation is the significant cell death rate of implanted cells after being grafted into injured heart. Therefore, overcoming the poor survival rate of implanted cells may improve stem cell therapy. Due to limited improvement concerning direct stem cell therapy, gene-transfer methods are used to enhance cellular cardiomyoplasty efficacy. Heme oxygenase-1 (HO-1) can provide various types of cells with protection against oxidative injury and apoptosis. However, exact effects of autologous ADSCs combined with HO-1 on cardiac performance remains unknown. In this study, rabbits were treated with ADSCs transduced with HO-1 (HO-1-ADSCs), treated with non-transduced ADSCs, or injected with phosphate buffered saline 14 days after experimental myocardial infarction was induced, when autologous ADSCs were obtained simultaneously. Four weeks after injection, echocardiography showed significant improvements for cardiac functions and left ventricular dimensions in HO-1-ADSCs-treated animals. Structural consequences of transplantation were determined by detailed histological analysis, which showed differentiation of HO-1-ADSCs to cardiomyocyte-like tissues and lumen-like structure organizations. Apart from improvement in angiogenesis and scar areas, more connexin 43-positive gap junction and greater tyrosine hydroxylase-positive cardiac sympathetic nerves sprouting were observed in the HO-1-ADSCs-treated group compared with ADSCs group. These data suggest that the transplantation of autologous ADSCs combined with HO-1 transduction is a feasible and efficacious method for improving infarcted myocardium.

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confidence: 99%

Transplantation of Adipose Tissue-Derived Stem Cells Overexpressing Heme Oxygenase-1 Improves Functions and Remodeling of Infarcted Myocardium in Rabbits

Yang

Liu

et al. 2012

Tohoku J. Exp. Med.

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“…A large non-reperfused infarction was induced by permanent coronary artery ligation as described [20,21]. Briefly, after being anaesthetized with urethane (1.0 -1.2 g/kg), male C57BL/6 mice were intubated, placed in the right lateral decubitus position, subjected to left thoracotomy, pericardiectomy and ligation of the left anterior descending coronary artery, and after closure of the thorax placed in the prone position until spontaneous breathing occurred.…”

Section: Induction Of Amimentioning

confidence: 99%

“…Myocardial infarct size was determined by Evans blue-TTC double staining methods as previously described [20]. Briefly, Evan's blue dye (2%; Sigma) was intravenously injected to delineate the in vivo AAR, and the heart was rapidly excised and rinsed in 0.9% NaCl.…”

Section: Assessment Of Area At Risk (Aar) and Infarct Size (Is)mentioning

confidence: 99%

Anti-CCL21 Antibody Attenuates Infarct Size and Improves Cardiac Remodeling After Myocardial Infarction

Jiang

Bai²,

Tang³

et al. 2015

Cell Physiol Biochem

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Background/Aims: Over-activation of cellular inflammatory effectors adversely affects myocardial function after acute myocardial infarction (AMI). The CC-chemokine CCL21 is, via its receptor CCR7, one of the key regulators of inflammation and immune cell recruitment, participates in various inflammatory disorders, including cardiovascular ones. This study explored the therapeutic effect of an anti-CCL21 antibody in cardiac remodeling after myocardial infarction. Methods and Results: An animal model of AMI generated by left anterior descending coronary artery ligation in C57BL/6 mice resulted in higher levels of circulating CCL21 and cardiac CCR7. Neutralization of CCL21 by intravenous injection of anti-CCL21 monoclonal antibody reduced infarct size after AMI, decreased serum levels of neutrophil and monocyte chemo attractants post AMI, diminished neutrophil and macrophage recruitment in infarcted myocardium, and suppressed MMP-9 and total collagen content in myocardium. Anti-CCL21 treatment also limited cardiac enlargement and improved left ventricular function. Conclusions: Our study indicated that CCL21 was involved in cardiac remodeling post infarction and anti-CCL21 strategies might be useful in the treatment of AMI.

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“…Adipose tissue-derived MSCs have been delivered via intramyocardial injection immediately after MI induction in mouse and pig models (Alt et al, 2010;Yu et al, 2010). In animal studies these cells have been shown to increase the vessel density, reduced scar size and induce a functional improvement, as evidenced by increased left ventricular ejection fraction (LVEF) and fractional shortening (FS).…”

Section: Resident Adult Stem Cells (Ascs)mentioning

confidence: 99%

Stem cell therapy for cardiovascular disease: the demise of alchemy and rise of pharmacology

Jadczyk

Faulkner

Madeddu

2013

British J Pharmacology

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Regenerative medicine holds great promise as a way of addressing the limitations of current treatments of ischaemic disease. In preclinical models, transplantation of different types of stem cells or progenitor cells results in improved recovery from ischaemia. Furthermore, experimental studies indicate that cell therapy influences a spectrum of processes, including neovascularization and cardiomyogenesis as well as inflammation, apoptosis and interstitial fibrosis. Thus, distinct strategies might be required for specific regenerative needs. Nonetheless, clinical studies have so far investigated a relatively small number of options, focusing mainly on the use of bone marrow-derived cells. Rapid clinical translation resulted in a number of small clinical trials that do not have sufficient power to address the therapeutic potential of the new approach. Moreover, full exploitation has been hindered so far by the absence of a solid theoretical framework and inadequate development plans. This article reviews the current knowledge on cell therapy and proposes a model theory for interpretation of experimental and clinical outcomes from a pharmacological perspective. Eventually, with an increased association between cell therapy and traditional pharmacotherapy, we will soon need to adopt a unified theory for understanding how the two practices additively interact for a patient's benefit. LINKED ARTICLESThis article is part of a themed section on Regenerative Medicine and Pharmacology: A Look to the Future. To view the other articles in this section visit http://dx

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Improvement of cardiac function and remodeling by transplanting adipose tissue-derived stromal cells into a mouse model of acute myocardial infarction

Cited by 40 publications

References 20 publications

Transplantation of Adipose Tissue-Derived Stem Cells Overexpressing Heme Oxygenase-1 Improves Functions and Remodeling of Infarcted Myocardium in Rabbits

Transplantation of Adipose Tissue-Derived Stem Cells Overexpressing Heme Oxygenase-1 Improves Functions and Remodeling of Infarcted Myocardium in Rabbits

Anti-CCL21 Antibody Attenuates Infarct Size and Improves Cardiac Remodeling After Myocardial Infarction

Stem cell therapy for cardiovascular disease: the demise of alchemy and rise of pharmacology

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