Improvement of chemical analysis of antibiotics

Oka, Hisao; Uno, Keiichi; Harada, Kenichi; Hayashi, Mika; Suzuki, Makoto

doi:10.1016/s0021-9673(01)87604-4

Cited by 14 publications

(6 citation statements)

References 9 publications

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“…Among them HPLC coupled with UV [10 -13], MS [14 -17], or electrochemical detector [18] is the most widely used technique for multiresidue analysis of TCs in milk. TLC can also be used as a screening method for the analysis of TCs in milk [19,20].…”

Section: Introductionmentioning

confidence: 99%

“…Due to these characteristics, a deproteinizing agent and a mild acidic extraction buffer are demanded for the isolation of TCs from milk samples. McIlvaine buffer (citric acid with disodium hydrogen phosphate) [10,12,19], succinate [14,13], and acetate [16] buffers are used as extracting solvents, while TCA [10] and HCl [18] as deproteinizing agents.…”

Section: Introductionmentioning

confidence: 99%

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Development and validation of an HPLC confirmatory method for the determination of seven tetracycline antibiotics residues in milk according to the European Union Decision 2002/657/EC

Samanidou

Nikolaidou

Papadoyannis³

2007

J of Separation Science

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An HPLC method with diode-array detection, at 355 nm, was developed and validated for the determination of seven tetracyclines (TCs) in milk: minocycline (MNC), TC, oxytetracycline (OTC), methacycline (MTC), demeclocycline (DMC), chlortetracycline (CTC), and doxycycline (DC). Oxalate buffer (pH 4) was used with 20% TCA as a deproteinization agent for the extraction of analytes from milk followed by SPE. The separation was achieved on an Inertsil ODS-3, 5 microm, 250 x 4 mm(2 )analytical column at ambient temperature. The mobile phase, a mixture of A: 0.01 M oxalic acid and B: CH(3)CN, was delivered using a gradient program. The procedure was validated according to the European Union decision 2002/657/EC determining selectivity, stability, decision limit, detection capability, accuracy, and precision. Mean recoveries of TCs from spiked milk samples (50, 100, and 200 ng/g) were 93.8-100.9% for MNC, 96.8-103.7% for OTC, 96.3-101.8% for TC, 99.4-107.2% for DMC, 99.4-102.9% for CTC, 96.3-102.7% for MTC, and 94.6-102.1% for DC. All RSD values were lower than 8.5%. The decision limits CC(a) calculated by spiking 20 blank milk samples at MRL (100 microg/kg) ranged from 101.25 to 105.84 microg/kg, while detection capability CC(b )from 103.94 to 108.88 microg/kg.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development and validation of an HPLC confirmatory method for the determination of seven tetracycline antibiotics residues in milk according to the European Union Decision 2002/657/EC

Samanidou

Nikolaidou

Papadoyannis³

2007

J of Separation Science

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“…Ninhydrin (Claes, 1982), fast violet (Oka, 1984) etc. have been used as detection reagents for antibiotics; these are considered to have certain disadvantages, e.g.…”

Section: Resultsmentioning

confidence: 99%

TLC separation of certain tetracycline and amino glycopeptide antibiotics

Bhushan

Ali

1992

Biomedical Chromatography

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The separation of tetracycline and amino glycopeptide antibiotics was achieved on silica gel thin layers. Tetracycline antibiotics were resolved on a Co+2 (1.0%) impregnated silica gel layer using ethanol:acetic acid:water (10:6:6, v/v/v) as the mobile phase. Amino glycopeptide antibiotics were separated on an untreated silica gel layer using the mobile phase n-butanol:formic acid:water (6:5:7, v/v/v). The spots of these antibiotics were located by exposing the chromatoplate to iodine vapours.

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“…Numerous natural phenolic products, including cannabinoids (114), vitamin E (115), morphine alkaloids (116), toxins such as gossypol (117), tetracyclines (118), phenolic steroids (119) rapeseed oil (120a), salicin in the bark of willow (120b), anthocyanins (121), and other natural product groups have all been studied by TLC. The identification of substances separated by TLC can be achieved reliably only by chemical and spectroscopic means.…”

confidence: 99%

Phenols, Aromatic Carboxylic Acids, and Indoles

Tyman¹

2003

Handbook of Thin-Layer Chromatography

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Improvement of chemical analysis of antibiotics

Cited by 14 publications

References 9 publications

Development and validation of an HPLC confirmatory method for the determination of seven tetracycline antibiotics residues in milk according to the European Union Decision 2002/657/EC

Development and validation of an HPLC confirmatory method for the determination of seven tetracycline antibiotics residues in milk according to the European Union Decision 2002/657/EC

TLC separation of certain tetracycline and amino glycopeptide antibiotics

Phenols, Aromatic Carboxylic Acids, and Indoles

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