Improvement of chondroitinases ABCI stability in natural deep eutectic solvents

Daneshjou, Sara; Khodaverdian, Shima; Dabirmanesh, Bahareh; Rahimi, Firoozeh; Daneshjoo, Somayeh; Ghazi, Farideh; Khajeh, Khosro

doi:10.1016/j.molliq.2016.11.130

Cited by 46 publications

(17 citation statements)

References 31 publications

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“…Moreover, Penicillium expansum lipase was found to be activated and stabilized up to 2.4 times and 18.4 times, respectively, in NADESs‐containing cosolvents, which was attributed to their complex hydrogen (H)‐bonding interactions . Similarly, 60% and 40% residual activity of ondroitinases ABCI was obtained in choline chloride‐glycerol and betaine‐glycerol based NADESs, respectively, after treatment for 120 min at 333.15 K . Obviously, although NADESs can act in the role of the enzyme, or more especially lipase, activator and stabilizer, the mechanism by which NADESs express these attractive advantages remain ambiguous, leading to the focus of the present study.…”

Section: Introductionmentioning

confidence: 98%

How Candida antarctica lipase B can be activated in natural deep eutectic solvents: experimental and molecular dynamics studies

Nian

Cao

Liu

2019

J of Chemical Tech & Biotech

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BACKGROUND: Natural deep eutectic solvents (NADESs), a class of green solvents which completely accords to 12 principles of green chemistry, have proven to have great potential applications in enzymatic reactions. Despite strong interest, the role of NADESs in these processes, and the molecular interaction between enzymes and NADESs, still remain ambiguous. In the present study, the stability and activity of Candida Antarctica lipase B (CALB) were studied, and the mechanism by which CALB was activated in NADESs was explored systematically from both molecular-and macroscopic-scale perspectives. RESULTS:The results suggested that the activity of CALB in all NADESs was significantly higher than that of the control (ethanol). Moreover, the stability of CALB increased to 115.48 ± 1.36% and 108.54 ± 1.26% in betaine-glycerin (B-Gly) and choline chloride-glycerol (C-Gly), but decreased to 91.69 ± 3.26% and 92.31 ± 3.36% in betaine-xylitol (B-X) and choline chloride-xylitol (C-X), respectively. The results of circular dichroism (CD), fluorescence spectroscopy and molecular dynamics studies (MD) indicated that there was no significant change in the secondary structure of CALB. Furthermore, the results of MD provide some information supporting that CALB was stabilized by the hydrogen(H)-bonding interaction between surface amino residues of CALB and NADESs and was activated via the H-bonding interaction between substrate and NADESs in the acyl-binding pocket. CONCLUSION: The mechanism by which CALB was activated and stabilized via the H-bonding interactions in NADESs wasrevealed in the present study. This provides a scientific basis from which to further explore the potential application of NADESs in enzymatic reactions in food engineering and health-related fields.

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Section: Introductionmentioning

confidence: 98%

How Candida antarctica lipase B can be activated in natural deep eutectic solvents: experimental and molecular dynamics studies

Nian

Cao

Liu

2019

J of Chemical Tech & Biotech

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“…Glyceline can be used in many applications. For instance, in catalytic reactions of compounds with therapeutic properties, such as N-phenyl phthalimide derivatives, which are used as anticonvulsants and anti-inflammatory drugs [19] or extraction [20] and enzyme stabilization agent [21]. Other applications are related with the purification of (bio)fuels [22][23] or absorption processes of CO2 and SO2 [24] Besides, different studies about the (eco)toxicity of Gly have shown that it is relatively safe for human health and environment [25].…”

Section: Introductionmentioning

confidence: 99%

The NADES glyceline as a potential Green Solvent: A comprehensive study of its thermophysical properties and effect of water inclusion

Lapeña

Lomba

Artal

et al. 2019

The Journal of Chemical Thermodynamics

100

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In this paper, two Natural Deep Eutectic Solvents, glyceline (Gly) and glyceline-water (GlyW), containing choline chloride as acceptor H-bond compound and glycerol as donor H-bond group are studied. For glyceline the mole relation is 1 (choline chloride): 2 (glycerol) and for glyceline-water the mole relation is 1 (choline chloride) : 1.99 (glycerol) : 1.02 water. The ternary NADES has been synthetized and characterized chemically by NMR techniques for this work. Several thermophysical properties in a wide range of temperature (278.15-338.15) K and at atmospheric pressure (0.1 MPa) have been measured for both compounds: density, , speed of sound, , refractive index, , surface tension, , isobaric molar heat capacity, , , kinematic viscosity, , and electric conductivity,. Furthermore, some related properties have been also calculated: isobaric expansibility, , isentropic compressibility, , molar refraction, , entropy and enthalpy of surface formationper unit surface area (Δ and Δ), and dynamic viscosity, , and viscous flow and electrical conductivity activation energies. The results have been discussed in terms of the effect of temperature and the inclusion of water. We conclude that the compound containing water into the structurehas a higher molar volume and a higher fluidity. The binary NADES (Gly) is a more structured liquid than ternary one (GlyW).

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“…A millimolar absorption coefficient of 5.1 L/(mol·cm) was used in the calculations. One international unit was defined as the amount of protein needed to form 1 µmol of 4,5-unsaturated uronic acid/min [25].…”

Section: Methodsmentioning

confidence: 99%

Expression, Purification and Characterization of Chondroitinase AC II from Marine Bacterium Arthrobacter sp. CS01

Fang

Yang

et al. 2019

Marine Drugs

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Chondroitinase (ChSase), a type of glycosaminoglycan (GAG) lyase, can degrade chondroitin sulfate (CS) to unsaturate oligosaccharides, with various functional activities. In this study, ChSase AC II from a newly isolated marine bacterium Arthrobacter sp. CS01 was cloned, expressed in Pichia pastoris X33, purified, and characterized. ChSase AC II, with a molecular weight of approximately 100 kDa and a specific activity of 18.7 U/mg, showed the highest activity at 37 °C and pH 6.5 and maintained stability at a broad range of pH (5–7.5) and temperature (below 35 °C). The enzyme activity was increased in the presence of Mn2+ and was strongly inhibited by Hg2+. Moreover, the kinetic parameters of ChSase AC II against CS-A, CS-C, and HA were determined. TLC and ESI-MS analysis of the degradation products indicated that ChSase AC II displayed an exolytic action mode and completely hydrolyzed three substrates into oligosaccharides with low degrees of polymerization (DPs). All these features make ChSase AC II a promising candidate for the full use of GAG to produce oligosaccharides.

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Improvement of chondroitinases ABCI stability in natural deep eutectic solvents

Cited by 46 publications

References 31 publications

How Candida antarctica lipase B can be activated in natural deep eutectic solvents: experimental and molecular dynamics studies

How Candida antarctica lipase B can be activated in natural deep eutectic solvents: experimental and molecular dynamics studies

The NADES glyceline as a potential Green Solvent: A comprehensive study of its thermophysical properties and effect of water inclusion

Expression, Purification and Characterization of Chondroitinase AC II from Marine Bacterium Arthrobacter sp. CS01

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