Improvement of conditions for the determination of neurotransmitters in rat brain tissue by HPLC with fluorimetric detection

Nováková, Dana; Kudláček, Karel; Novotný, Jiřı́; Nesměrák, Karel

doi:10.1007/s00706-022-02924-w

Cited by 7 publications

(7 citation statements)

References 20 publications

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“…Due to the unneglected physiological effects of DA, its precise detection is crucial for the clinical diagnosis of related diseases. Several methods have been employed for DA detection, including high-performance liquid chromatography (HPLC) [5], electrochemical methods [6,7], and colorimetry [8,9]. However, these methods are limited to time-consuming, expensive, low selectivity and so on.…”

Section: Introductionmentioning

confidence: 99%

Ratiometric fluorescence detection of dopamine based on copper nanoclusters and carbon dots

Meng,

Sun,

et al. 2024

Nanotechnology

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Nanoclusters for fluorescence detection are generally comprised of rare and expensive noble metals, and the nanoclusters based on more affordable transition metal have attracted increasing attention. This study designed a ratiometric fluorescent probe to detect dopamine (DA), an important neurotransmitter. With carbon dots encapsulated within silica (CDs@SiO2) as the reference, the emitted reference signal was almost unchanged due to the protection of inert silicon shell. Meanwhile, copper nanoclusters modified with 3-aminophenyl boronic acid (APBA-GSH-CuNCs) provided the sensing signal, in which the phenylboric acid could specifically recognize the cis-diol structure of DA, and caused the fluorescence quenching by photoinduced electron transfer. This dual emission ratiometric fluorescent probe exhibited high sensitivity and anti-interference, and was able to selectively responded to DA with a linear range of 0~1.4 mM, the detection limit of 5.6 nM, and the sensitivity of 815 mM-1. Furthermore, the probe successfully detected DA in human serum samples, yielding recoveries ranging from 92.5% to 102.7%. Overall, this study highlights the promising potential of this ratiometric probe for detecting DA.

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Section: Introductionmentioning

confidence: 99%

Ratiometric fluorescence detection of dopamine based on copper nanoclusters and carbon dots

Meng,

Sun,

et al. 2024

Nanotechnology

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“…[22][23][24][25] Therefore, Identifying and quantifying catechol aldehydes is crucial for the early detection of neurological and cardiovascular disorders. However, traditional detection methods such as high-pressure liquid chromatography (HPLC), 26 mass spectroscopy (MS), 27 microdialysis, 28 capillary electrophoresis (CE), 29 electrochemical analysis, 30 and optical spectroscopy 31 are time-consuming, lack selectivity, require large sample volumes, and are not optimal for efficient detection. 32 Moreover, these techniques necessitate cell destruction, leading to inaccuracies due to oxidative stress-related artifacts.…”

Section: Introductionmentioning

confidence: 99%

Selective Sensing of Catechol Aldehydes Levels in Living Systems using FLIM-FRET

Talbott,

Wills,

Shirke

et al. 2024

Preprint

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Endogenous catechol aldehydes (CAs), namely 3,4-dihydroxyphenylacetaldehyde (DOPAL) and 3,4-dihydroxyphenylglycolaldehyde (DOPEGAL), play pivotal roles in neurobehavioral, cardiovascular, and metabolic processes. Dysregulation of CA levels contributes to neurological disorders and heart diseases. Thus, detecting imbalances in CAs levels is crucial for diagnosing early stages of CA-associated diseases. Here, we present innovative fluorescent sensors designed for rapid and selective detection of CAs within cells and tissue, overcoming the limitations of conventional diagnostic methods that necessitate cell destruction. The sensor operates by a dual-reaction trigger, leveraging the exceptional selectivity of o-phenylenediamine for aldehyde and phenylboronic acid for catechol, resulting in the production of a FRET signal exclusively for CAs in the presence of other aldehydes and catechols within cells. To circumvent issues such as spectral cross-talk, excitation intensity fluctuations, inner filtering, photobleaching, and detector sensitivity, we employed Fluorescence Lifetime Imaging Microscopy (FLIM) combined with FRET (FLIM-FRET) to accurately measure CAs levels at a nanosecond scale. This makes FLIM-FRET highly proficient for live cell and tissue imaging. Remarkably, we utilized this dual-reaction trigger FLIM-FRET system to detect endogenous CAs levels within cells in response to enzyme activators and inhibitors and within diseased-model mice tissue. These probes have the potential to serve as early warning systems for neurological diseases linked to CAs within living systems, laying the foundation for further investigations.

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“…The use of many analytical techniques to determine NTs in biological samples has been developed. These techniques include chromatography with fluorescence [ 21 , 22 ], UV–visible spectroscopy [ 23 ], electrochemistry [ 24 , 25 ], high-performance liquid chromatography [ 26 ], and high-performance liquid chromatography (HPLC)–mass spectrometry (MS) [ 27 , 28 , 29 ]. Liquid chromatography–tandem mass spectrometry (LC-MS/MS) is increasingly becoming the method of choice for NT analysis due to its excellent sensitivity, specificity, and separation capability for complex samples [ 30 , 31 ].…”

Section: Introductionmentioning

confidence: 99%

Determination of Multiple Neurotransmitters through LC-MS/MS to Confirm the Therapeutic Effects of Althaea rosea Flower on TTX-Intoxicated Rats

Wang

Zheng

et al. 2023

Molecules

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Tetrodotoxin (TTX) inhibits neurotransmission in animals, and there is no specific antidote. In clinical practice in China, Althaea rosea (A. rosea flower) extract has been used to treat TTX poisoning. In this work, the efficacy of the ethyl acetate fraction extract of A. rosea flower in treating TTX poisoning in rats was investigated. A high-performance liquid chromatography–tandem mass spectrometry (LC-MS/MS) method was developed to determine nine neurotransmitters in rat brain tissue, including γ-aminobutyric acid (GABA), dopamine (DA), 5-hydroxytryptamine (5-HT), noradrenaline (NE), 3,4-dihydroxyphenylacetic acid (DOPAC), homovanillic acid (HVA), 5-hydroxyindole-3-acetic acid (5-HIAA), epinephrine (E), and tyramine (Tyn). The detoxifying effect of A. rosea flower was verified by comparing the changes in neurotransmitters’ content in brain tissue before and after poisoning in rats. The assay was performed in multiple reaction monitoring mode. The quantification method was performed by plotting an internal-standard working curve with good linearity (R2 > 0.9941) and sensitivity. Analyte recoveries were 94.04–107.53% (RSD < 4.21%). Results indicated that the levels of 5-HT, DA, E, and NE in the brains of TTX-intoxicated rats decreased, whereas the levels of GABA, Tyn, and 5-HIAA showed an opposite trend, and HVA and DOPAC were not detected. The levels of all seven neurotransmitters returned to normal after the gavage administration of ethyl acetate extract of A. rosea flower to prove that the ethyl acetate extract of A. rosea flower had a therapeutic effect on TTX poisoning. The work provided new ideas for studies on TTX detoxification.

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Improvement of conditions for the determination of neurotransmitters in rat brain tissue by HPLC with fluorimetric detection

Cited by 7 publications

References 20 publications

Ratiometric fluorescence detection of dopamine based on copper nanoclusters and carbon dots

Ratiometric fluorescence detection of dopamine based on copper nanoclusters and carbon dots

Selective Sensing of Catechol Aldehydes Levels in Living Systems using FLIM-FRET

Determination of Multiple Neurotransmitters through LC-MS/MS to Confirm the Therapeutic Effects of Althaea rosea Flower on TTX-Intoxicated Rats

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