Improvement of cryopreservation of Lactobacillus delbrueckii subsp. bulgaricus CFL1 with additives displaying different protective effects

Influence of fermentation time, cryoprotectant and neutralization of cell concentrate on freeze-drying survival, storage stability, and acid and bile exposure of Bifidobacterium animalis ssp. lactis cells produced without milk-based ingredients

“…Furthermore, when added as a cryoprotectant after growth it protected L. delbrueckii ssp. bulgaricus during freezing and frozen storage (Fonseca et al. 2003).…”

“…1993; Brashears and Gilliland 1995; Palmfeldt and Hahn‐Hägerdal 2000; Panoff et al. 2000; Fonseca et al. 2003; Carvalho et al.…”

Preliminary technological and potential probiotic characterisation of bifidobacteria isolated from breast milk for use in dairy products

“…However, the bacterial stability to the freezing process and to frozen storage is highly dependent on conditions of fermentation, stabilisation (concentration, cryoprotection, freezing) and storage (Fonseca, Béal, Mihoub, Marin, & Corrieu, 2003). Once isolated and cultured in laboratory culture media, we were interested in knowing the storage capacity of these bifidobacteria as concentrated frozen cultures.…”

Cryotolerance of Lactobacillus delbrueckii subsp. bulgaricus CFL1 is influenced by the physiological state during fermentation

“…Indeed in samples taken early in the fermentation, the medium should contain a higher level of unfermented lactose and a lower lactate content which should provide a better freezing medium than those taken later in the process. Therefore, even if the supernatant in which cells were resuspended during the log phase still contained some nutrients such as sugars and nitrogen sources that are known to play a cryoprotective role (Berner & Viernstein, 2006;Fonseca et al, 2003), this phenomenon was insufficient to improve cell cryotolerance, as compared with the late stationary phase supernatant that displayed nutritional deficiency and contained more toxic compounds such as lactate or lactic acid.…”

“…Specific cell concentration conditions (Streit, Corrieu, & Béal, 2008a), well-defined cryoprotectants (Carcoba & Rodriguez, 2000;Fonseca, Béal, Mihoub, Marin, & Corrieu, 2003), and specific freezing rates and storage temperatures (Bâati, Fabre-Gea, Auriol, & Blanc, 2000;Fonseca et al, 2006) limit viability losses. The application of cold (Bâati et al, 2000;Wang, Delettre, Guillot, Corrieu, & Béal, 2005b) or acid (Streit, Delettre, Corrieu, & Béal, 2008b) adaptation of the cells just before freezing also improved their cryotolerance.…”