Improvement of cultivation medium for enhanced production of coenzyme Q10 by photosynthetic Rhodospirillum rubrum

Tian, Yuting; Yue, Tianli; Yuan, Yahong; Soma, Pavan Kumar; Lo, Y. Martin

doi:10.1016/j.bej.2010.06.011

Cited by 23 publications

(11 citation statements)

References 44 publications

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“…Metabolic engineering strategies like the introduction of heterologous genes involved in the mevalonate pathway, [18] varying the NADH=NAD þ ratio, [35] limited dissolved oxygen supply, [37] and optimization of feeding strategies for decaprenyl diphosphate synthase gene from A. tumefaciens in Escherichia coli produced higher CoQ10 than wild-type strains. Despite a lower CoQ10 production than the aforesaid and earlier reports, [33,37,38] this study was first of its kind to report CoQ10 production by the less exploited red yeast R. glutinis using better optimized feeding strategies. Generally, coenzyme Q10 biosynthesis in yeast requires Coq polypeptide complex.…”

Section: Modeling Process Parameters For Production Of Coq10contrasting

confidence: 46%

“…Bule and Singhal [31] reported that optimization of media components by RSM in Pseudomonas diminuta increased CoQ10 production from 6.68 mg=L to 15.58 mg=L. Also, similar studies on medium optimization were carried out in the photosynthetic Rhodospirillum rubrum [33] and a yield …”

mentioning

confidence: 79%

“…[33] In this context, we designed two fed-batch cultivations, one with the feeding of the carbon source glucose, and the other with …”

Section: Fed-batch Cultivationmentioning

confidence: 99%

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Modeling of Process Parameters for Enhanced Production of Coenzyme Q10 FromRhodotorula glutinis

Balakumaran

Meenakshisundaram

2014

Preparative Biochemistry and Biotechnology

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Coenzyme Q10 (CoQ10) plays an indispensable role in ATP generation through oxidative phosphorylation and helps in scavenging superoxides generated during electron transfer reactions. It finds extensive applications specifically related to oxidative damage and metabolic dysfunctions. This article reports the use of a statistical approach to optimize the concentration of key variables for the enhanced production of CoQ10 by Rhodotorula glutinis in a lab-scale fermenter. The culture conditions that promote optimum growth and CoQ10 production were optimized and the interaction of significant variables para-hydroxybenzoic acid (PHB, 819.34 mg/L) and soybean oil (7.78% [v/v]) was studied using response surface methodology (RSM). CoQ10 production increased considerably from 10 mg/L (in control) to 39.2 mg/L in batch mode with RSM-optimized precursor concentration. In the fed-batch mode, PHB and soybean oil feeding strategy enhanced CoQ10 production to 78.2 mg/L.

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Section: Modeling Process Parameters For Production Of Coq10contrasting

confidence: 46%

mentioning

confidence: 79%

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Modeling of Process Parameters for Enhanced Production of Coenzyme Q10 FromRhodotorula glutinis

Balakumaran

Meenakshisundaram

2014

Preparative Biochemistry and Biotechnology

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“…COD removal by PSB can reach 60-95%. Furthermore, the PSB biomass contains many nutrients and physiologically active substances [9,10], which can be used as the supplement in fertilizer [11], feed [12], and bait [13]. Therefore, PSB wastewater treatment can realize pollutant removal and useful biomass accumulation simultaneously.…”

Section: Introductionmentioning

confidence: 99%

Optimization of photosynthetic bacteria wastewater treatment and study of microbial species diversity

Zhao

Zhang

2014

Desalination and Water Treatment

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A B S T R A C TThe photosynthetic bacteria (PSB) wastewater treatment was optimized in terms of light and oxygen conditions, pH, temperature, initial PSB, and wastewater concentrations. The changes of microbial species were studied using PCR-DGGE technique. The results showed that in batch reactors the optimum conditions of PSB soybean wastewater treatment were natural light-micro oxygen, initial PSB concentration of 160 mg/L, initial wastewater concentration of 3,600 mg/L, pH of 8.0, and temperature of 30˚C. In semi-continuous reactors, the results verified that under the optimum conditions PSB soybean wastewater treatment was effective and PSB biomass increase was good. PCR-DGGE analyses showed that the types of microorganisms in the reactor gradually increased. Under the optimum conditions, Lactococcus lactis subsp. formed on the 7 th day; Veillonella sp. and Dysgonomonas sp. formed on the 14 th day; and iron-reducing bacterium and Veillonella sp. formed and Lactococcus lactis subsp. disappeared on the 21 st day; Janthinobacterium lividum formed on the 28 th day; and the community did not change from the 28 th to 35 th day; after that the system entered a stable situation. PSB were the dominate bacteria during the whole period.

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“…The effect of type and concentration of carbon and nitrogen source on CoQ 10 fermentation kinetics parameters using Paracoccus denitrificans was studied in order to optimize the nutritional requirement [32]. The improvement in cultivation medium for enhanced production of CoQ 10 by photosynthetic R. rubrum using response surface methodology was reported [36]. Ca 2+ increases the specific CoQ 10 content in Agrobacterium tumefaciens was also reported [37].…”

Section: Optimization Of Production Mediamentioning

confidence: 99%

Influence of Substrate Feeding and Process Parameters on Production of Coenzyme Q<sub>10</sub> Using <i>Paracoccus denitrificans</i> ATCC 19367 Mutant Strain P-87

Tokdar¹,

Ranadive²,

Kshirsagar³

et al. 2014

ABB

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Coenzyme Q 10 (CoQ 10 ), an important antioxidant molecule playing a major role in electron transport chain, has been commercially produced by fermentation process for the use in oral nutraceutical formulations. Constructing the high-yielding CoQ 10 producing strains is a pre-requisite for cost-effective production. A superior mutant strain P-87 generated from Paracoccus denitrificans ATCC 19367, which showed 1.25-fold improvement in specific CoQ 10 content higher than the wild type strain at shake flask level, was selected to carry out the studies on CoQ 10 yield improvement through fermenter process optimization. In the course of study, initially the cane-molasses-based medium and fed-batch fermentation strategy using pHBA in combination with sucrose were standardized in shake flask using wild type strain. This strategy was subsequently translated at 2 L laboratory fermenter while optimizing the fermentation process parameters using improved mutant strain P-87. Under optimized fermentation condition, mutant strain P-87 produced 49.85 mg/L of CoQ10 having specific content of 1.63 mg/g of DCW, which was 1.36 folds higher than the specific CoQ 10 content of wild-type strain under similar optimized condition. The temperature and DO were found to be critical parameters for CoQ 10 production by mutant strain P-87. The optimum temperature was found to be 32˚C and the optimum DO concentration to be maintained throughout the fermentation cycle was found to be 30% of air saturation. Overall, a new cost-effective process has been established for the production of CoQ 10 using the cheaper substrate "cane molasses" and higher CoQ 10 producing mutant strain P-87. *Corresponding author. P. Tokdar et al.967

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Improvement of cultivation medium for enhanced production of coenzyme Q10 by photosynthetic Rhodospirillum rubrum

Cited by 23 publications

References 44 publications

Modeling of Process Parameters for Enhanced Production of Coenzyme Q10 FromRhodotorula glutinis

Modeling of Process Parameters for Enhanced Production of Coenzyme Q10 FromRhodotorula glutinis

Optimization of photosynthetic bacteria wastewater treatment and study of microbial species diversity

Influence of Substrate Feeding and Process Parameters on Production of Coenzyme Q<sub>10</sub> Using <i>Paracoccus denitrificans</i> ATCC 19367 Mutant Strain P-87

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Improvement of cultivation medium for enhanced production of coenzyme Q10 by photosynthetic Rhodospirillum rubrum

Cited by 23 publications

References 44 publications

Modeling of Process Parameters for Enhanced Production of Coenzyme Q10 FromRhodotorula glutinis

Modeling of Process Parameters for Enhanced Production of Coenzyme Q10 FromRhodotorula glutinis

Optimization of photosynthetic bacteria wastewater treatment and study of microbial species diversity

Influence of Substrate Feeding and Process Parameters on Production of Coenzyme Q&lt;sub&gt;10&lt;/sub&gt; Using &lt;i&gt;Paracoccus denitrificans&lt;/i&gt; ATCC 19367 Mutant Strain P-87

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Influence of Substrate Feeding and Process Parameters on Production of Coenzyme Q<sub>10</sub> Using <i>Paracoccus denitrificans</i> ATCC 19367 Mutant Strain P-87