Improvement of Culture Conditions and Plant Growth Regulators for In Vitro Callus Induction and Plant Regeneration in Paeonia lactiflora Pall.

Abstract

Owing to its high ornamental, medicinal and horticultural values, herbaceous peony (Paeonia lactiflora Pall.) has been widely used as a landscaping and economical plant around the world. However, the lack of an efficient and stable regeneration system in P. lactiflora restricts its rapid propagation and large-scale production. By testing the key factors affecting callus formation, proliferation, adventitious bud induction and rooting, here, we developed an in vitro system for callus induction and regeneration in P. lactiflora. Our results show that callus formation was affected by explant types, culture environment, basal medium and plant growth regulators. Using cotyledons as explants, we established good conditions for P. lactiflora callus induction and callus proliferation. We effectively obtained adventitious buds differentiated from callus in Murashige and Skoog (MS) medium containing kinetin (KT) and thidiazuron (TDZ). Adventitious bud growth can be further promoted by adding gibberellin 3 (GA3), 1-naphthaleneacetic acid (NAA) and 6-benzyleaminopurine (6-BA) into the MS medium. A high percentage of rooting can be achieved by adding indolebutyric acid (IBA) and activated carbon (AC) to ½ MS medium. Overall, our system promotes callus induction and adventitious bud regeneration for P. lactiflora through improved culture conditions and plant growth regulators in the culture media, and lays a foundation for subsequent genetic engineering research.

In vitro propagation of stone fruit rootstock cultivar Evrica 99 and its influence on some phytochemical traits of fresh apricot fruit

“…This result is consistent with those reported by other researchers regarding the essential role of auxins in plant propagation by cuttings [52][53][54][55][56]. IBA was found to be the most effective in vitro rooting regulator of all three auxins studied, in agreement with results from other studies (57)(58). The results revealed that as the amount of auxin in the culture medium increased, the formation of calluses at the base of micro-shoots occurred.…”

An efficient in vitro organogenesis protocol for the endangered relic tree species Bretschneidera sinensis and genetic fidelity assessment using DNA markers

“…For Populus trichocarpa , a cytokinin/auxin ratio of 5/1 resulted in the highest bud induction rate ( Ma et al., 2022 ). However, the specific ratios and intensities of these hormones have not been clearly determined across different plants, and varying these ratios with different hormone strengths can lead to dramatically different outcomes ( Song et al., 2023 ). We observed both high and low induction rates at a 6-BA/NAA ratio of 3/1.…”