Improvement of cumin (Cuminum cyminum) seed performance under drought stress by seed coating and biopriming

Radish (Raphanus sativus L.) is a vegetable cultivated worldwide because of its large succulent hypocotyls. The priming method initiates metabolic processes at early stages and regulates the metabolic events in seed necessary for germination. This research was conducted to examine the influence of various priming treatments on physiological performance (germination, growth, lipid peroxidation, primary and secondary metabolism) and antioxidant activity of radish seedlings. On the basis of germination and growth characteristics, vigor index, and relative water content in leaves, it was confirmed that priming treatments with 0.01% ascorbic acid (AA) and 1% KNO3 improves the initial stages of radish development. Furthermore, the efficiency of AA as a priming agent was confirmed through the reduction of malondialdehyde (MDA) level compared to unprimed seedlings. On the other hand, hormopriming with indole-3-acetic acid (IAA) significantly increased the concentration of photosynthetic pigments and total soluble leaf proteins compared to non-primed seedlings. The highest content of total phenolic compounds, including flavonoids, were obtained after hormopriming with 1 mM IAA and halopriming with 1% MgSO4. On the basis of the percentage of inhibition of DPPH radicals, it was confirmed that treatments with IAA and AA can improve the antioxidant activity of radish seedlings. This study provides useful information regarding the possibilities of pregerminative metabolic modulation through the seed priming for the biochemical and physiological improvement of radish, and this topic should be further investigated in order to determine the potential use of AA and IAA as suitable priming agents in radish commercial production.

Section: Discussionsupporting

confidence: 92%

Improvement of Germination and Early Growth of Radish (Raphanus sativus L.) through Modulation of Seed Metabolic Processes

Kanjevac

Jakovljević

Todorović

et al. 2022

“…Considering cucumber seeds, Pill et al [70] applied a commercial preparation of T. harzianum as a coating agent obtaining higher seedling emergence and seedling shoot fresh weight with respect to uncoated seeds. The same Trichoderma species was effective for ameliorating germination and seedling growth of Cucurbita pepo under different salinity stresses (50 and 100 mM NaCl solution) [71] and of Cuminum cyminum under drought stress [72]. Piri et al [72] applied different T. harzianum strains or Pseudomonas fluorescence bacteria and observed an increase in soluble protein and antioxidant enzyme activity of inoculated seeds with respect to a control without inoculation leading to enhancement of some morphological indices.…”

Section: Other Horticultural Speciesmentioning

confidence: 99%

Seed Treatments with Microorganisms Can Have a Biostimulant Effect by Influencing Germination and Seedling Growth of Crops

Cardarelli

Woo

Rouphael

et al. 2022

Seed quality is an important aspect of the modern cultivation strategies since uniform germination and high seedling vigor contribute to successful establishment and crop performance. To enhance germination, beneficial microbes belonging to arbuscular mycorrhizal fungi, Trichoderma spp., rhizobia and other bacteria can be applied to seeds before sowing via coating or priming treatments. Their presence establishes early relationships with plants, leading to biostimulant effects such as plant-growth enhancement, increased nutrient uptake, and improved plant resilience to abiotic stress. This review aims to highlight the most significant results obtained for wheat, maize, rice, soybean, canola, sunflower, tomato, and other horticultural species. Beneficial microorganism treatments increased plant germination, seedling vigor, and biomass, as well as overcoming seed-related limitations (such as abiotic stress), both during and after emergence. The results are generally positive, but variable, so more scientific information needs to be acquired for different crops and cultivation techniques, with considerations to different beneficial microbes (species and strains) and under variable climate conditions to understand the effects of seed treatments.

“…Arable land is affected by a range of abiotic stress factors, such as drought, and they lead to reduced plant growth and ultimately low crop productivity. Seed biopriming is a promising approach to preventing the negative effects of water deficit on plant growth [8,23,24]. Indeed, plant resistance to abiotic stress can be improved significantly by seed biopriming, as it enhances gene expression related to the plant antioxidant system and plant metabolism to prevent oxidative and growth damage to achieve better yields [25].…”

Section: Introductionmentioning

confidence: 99%

Biopriming of Maize Seeds with a Novel Bacterial Strain SH-6 to Enhance Drought Tolerance in South Korea

Shaffique

Khan

Wani

et al. 2022

Maize is the third most common cereal crop worldwide, after rice and wheat, and plays a vital role in preventing global hunger crises. Approximately 50% of global crop yields are reduced by drought stress. Bacteria as biostimulants for biopriming can improve yield and enhance sustainable food production. Further, seed biopriming stimulates plant defense mechanisms. In this study, we isolated bacteria from the rhizosphere of Artemisia plants from Pohang beach, Daegu, South Korea. Twenty-three isolates were isolated and screened for growth promoting potential. Among them, bacterial isolate SH-6 was selected based on maximum induced tolerance to polyethylene glycol-simulated drought. SH-6 showed ABA concentration = 1.06 ± 0.04 ng/mL, phosphate solubilizing index = 3.7, and sucrose concentration = 0.51 ± 0.13 mg/mL. The novel isolate SH-6 markedly enhanced maize seedling tolerance to oxidative stress owing to the presence of superoxide dismutase, catalase, and ascorbate peroxidase activities in the culture media. Additionally, we quantified and standardized the biopriming effect of SH-6 on maize seeds. SH-6 significantly increased maize seedling drought tolerance by up to 20%, resulting in 80% germination potential. We concluded that the novel bacterium isolate SH-6 (gene accession number (OM757882) is a biostimulant that can improve germination performance under drought stress.