Improvement of desulfurizing activity of haloalkaliphilic Thialkalivibrio versutus SOB306 with the expression of Vitreoscilla hemoglobin gene

Mu, Tingzhen; Yang, Maohua; Zhao, Jixiang; Sharshar, Moustafa Mohammed; Tian, Jiangnan; Xing, Jianmin

doi:10.1007/s10529-016-2266-1

Cited by 10 publications

(9 citation statements)

References 13 publications

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“…In terms of the development of bacteria for desulfurization by metabolic engineering, expression of Vitreoscilla globin gene ( vgb ) and knockout of sulfate producing key gene hdrB improved desulfurizing activity using haloalkaliphilic T . versutus (Mu et al 2017 ; Sharshar et al 2020 ). Fed-batch culture coupled with design, build, test, and validate approach successfully led to the first tight inducible system construction in T .…”

Section: Discussionmentioning

confidence: 99%

“…The members of this genus are well adapted to hypersaline (up to salt saturation) and highly alkaline (up to pH 10.5) conditions and fix inorganic carbon as a carbon source (Sorokin et al 2006 ). It has been established that the Vitreoscilla hemoglobin (VHb) level in Thioalkalivibrio versutus D301 promoted thiosulfate oxidation (Mu et al 2017 ). The production of nanometric sulfur from sulfide was enhanced by 166.7 % in another T. versutus D301 mutant in which the conversion of sulfur to sulfate was blocked by deleting the critical hdrB gene (Sharshar et al 2020 ).…”

Section: Introductionmentioning

confidence: 99%

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Development of whole-cell catalyst system for sulfide biotreatment based on the engineered haloalkaliphilic bacterium

et al. 2021

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Microorganisms play an essential role in sulfide removal. Alkaline absorption solution facilitates the sulfide’s dissolution and oxidative degradation, so haloalkaliphile is a prospective source for environmental-friendly and cost-effective biodesulfurization. In this research, 484 sulfide oxidation genes were identified from the metagenomes of the soda-saline lakes and a haloalkaliphilic heterotrophic bacterium Halomonas salifodinae IM328 (=CGMCC 22183) was isolated from the same habitat as the host for expression of a representative sequence. The genetic manipulation was successfully achieved through the conjugation transformation method, and sulfide: quinone oxidoreductase gene (sqr) was expressed via pBBR1MCS derivative plasmid. Furthermore, a whole-cell catalyst system was developed by using the engineered strain that exhibited a higher rate of sulfide oxidation under the optimal alkaline pH of 9.0. The whole-cell catalyst could be recycled six times to maintain the sulfide oxidation rates from 41.451 to 80.216 µmol·min−1·g−1 dry cell mass. To summarize, a whole-cell catalyst system based on the engineered haloalkaliphilic bacterium is potentiated to be applied in the sulfide treatment at a reduced cost.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development of whole-cell catalyst system for sulfide biotreatment based on the engineered haloalkaliphilic bacterium

et al. 2021

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“…The native vgb promoter works in several Gram-negative bacteria, including eight-tandem vgb promoter P8vgb in E. coli , Halomonas bluephagenesis and Halomonas campaniensis [ 37 , 38 ]. The specific promoters that have been chosen for other bacteria include trc promoter in E. coli [ 39 , 40 ], tac promoter in E. coli and Thialkalivibrio versutus [ 41 , 42 , 43 ], P phaC1-j5 promoter in Cupriavidus necator [ 44 ], and P43 promoter in Bacillus subtilis [ 45 ]. Fungal promoters that have been used for expression in fungi include: tubulin promoter in Aurantiochytrium sp.…”

Section: The Heterologous Expression Of Vhbmentioning

confidence: 99%

“…Dependent on the function of sulfide receptor and storage [ 23 ], the expression of VHb in Rhodococcus erythropolis (desulfurization bacterium) presented a higher desulfurization ratio than the control ( vgb + -37.5% and vgb − -20.5%) under hypoxic conditions [ 83 ]. In the following, an increase of 11.7 ± 1.8% on the rate of thiosulfate scavenge was achieved in T. versutus by introducing the vgb gene [ 42 ]. In addition, the co-culture of desulfurization bacteria is also an effective sulfur degradation method [ 84 ].…”

Section: Applications Of Vhb In Biotechnologymentioning

confidence: 99%

Recent Advances in the Physicochemical Properties and Biotechnological Application of Vitreoscilla Hemoglobin

Zhao

Wang

et al. 2021

Microorganisms

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Vitreoscilla hemoglobin (VHb), the first discovered bacterial hemoglobin, is a soluble heme-binding protein with a faster rate of oxygen dissociation. Since it can enhance cell growth, product synthesis and stress tolerance, VHb has been widely applied in the field of metabolic engineering for microorganisms, plants, and animals. Especially under oxygen-limited conditions, VHb can interact with terminal oxidase to deliver enough oxygen to achieve high-cell-density fermentation. In recent years, with the development of bioinformatics and synthetic biology, several novel physicochemical properties and metabolic regulatory effects of VHb have been discovered and numerous strategies have been utilized to enhance the expression level of VHb in various hosts, which greatly promotes its applications in biotechnology. Thus, in this review, the new information regarding structure, function and expressional tactics for VHb is summarized to understand its latest applications and pave a new way for the future improvement of biosynthesis for other products.

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“…established a highly efficient genetic transformation system based on the conjugative transfer method in the genus Thialkalivibrio . Meanwhile, the average thiosulfate removal rate of T. versutus SOB306 was improved 11.7 ± 1.8% by expressing Vitreoscilla hemoglobin (VHb), because it utilizes dissolved O 2 more efficiently with the help of VHb [68]. With further research, Sharshar et al.…”

Section: Strategies For Improving the Microbial Capture Of H2s Using Sobmentioning

confidence: 99%

Recent advances in microbial capture of hydrogen sulfide from sour gas via sulfur‐oxidizing bacteria

Chen

Yang

Hao

et al. 2021

Engineering in Life Sciences

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Biological desulfurization offers several remarkably environmental advantages of operation at ambient temperature and atmospheric pressure, no demand of toxic chemicals as well as the formation of biologically re‐usable sulfur (S0), which has attracted increasing attention compared to conventionally physicochemical approaches in removing hydrogen sulfide from sour gas. However, the low biomass of SOB, the acidification of process solution, the recovery of SOB, and the selectivity of bio‐S0 limit its industrial application. Therefore, more efforts should be made in the improvement of the BDS process for its industrial application via different research perspectives. This review summarized the recent research advances in the microbial capture of hydrogen sulfide from sour gas based on strain modification, absorption enhancement, and bioreactor modification. Several efficient solutions to limitations for the BDS process were proposed, which paved the way for the future development of BDS industrialization.

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Improvement of desulfurizing activity of haloalkaliphilic Thialkalivibrio versutus SOB306 with the expression of Vitreoscilla hemoglobin gene

Abstract: A conjugation transfer and an expression system for Thialkalivibrio, has been developed for the first time and used for expression of VHb to improve desulfurizing activity.

Cited by 10 publications

References 13 publications

Development of whole-cell catalyst system for sulfide biotreatment based on the engineered haloalkaliphilic bacterium

Development of whole-cell catalyst system for sulfide biotreatment based on the engineered haloalkaliphilic bacterium

Recent Advances in the Physicochemical Properties and Biotechnological Application of Vitreoscilla Hemoglobin

Recent advances in microbial capture of hydrogen sulfide from sour gas via sulfur‐oxidizing bacteria

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