Improvement of Enterovirus Neutralization by Treatment with Sodium Deoxycholate or Chloroform

Kapsenberg, Jacoba G.; Ras, Albert; Korte, Jantje

doi:10.1159/000149092

Cited by 29 publications

(15 citation statements)

References 6 publications

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“…Identification of E30 was confirmed by an E30-specific RT-PCR (10) or a neutralization test with polyclonal antibodies (anti-E30 serum, ATCC VR-1072; anti-E4 serum, ATCC VR-1041). E30 was pretreated with a low concentration of chloroform to disaggregate the virus before performance of the neutralization test (9).…”

Section: Methodsmentioning

confidence: 99%

Laboratory Diagnosis and Genetic Analysis of an Echovirus 30-Associated Outbreak of Aseptic Meningitis in Taiwan in 2001

Wang

Tsai

Huang³

et al. 2002

J Clin Microbiol

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Section: Methodsmentioning

confidence: 99%

Laboratory Diagnosis and Genetic Analysis of an Echovirus 30-Associated Outbreak of Aseptic Meningitis in Taiwan in 2001

Wang

Tsai

Huang³

et al. 2002

J Clin Microbiol

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“…Isolates of enterovirus 71 are often difficult to neutralize, which has created problems in recognition of this important pathogen. Successful neutralization of the Swedish strains of enterovirus 71 depended on the use of monodispersed virus (5,45 (42) to be the treatment of choice for routine typing of enterovirus 71 and coxsackievirus types A7 and A16. Chloroform treatment was found to be effective with virus suspensions of low titer.…”

Section: Properties Of the Enterovirusesmentioning

confidence: 99%

My Role in the Discovery and Classification of the Enteroviruses

Melnick

1996

Annu. Rev. Microbiol.

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The enteroviruses constitute one of the genera of the picornavirus family. The genus includes the polioviruses, the coxsackieviruses, and the echoviruses of humans, plus a number of enteroviruses of lower animals (e.g. monkeys, cattle, pigs, mice). Over 100 serotypes are recognized, of which the first to be discovered were the polioviruses. It was my good fortune to have been a scientist during the golden age of virology, when new techniques were being introduced into the field. These often led to the discovery of new viruses. This article details the isolation of the enteroviruses, their recognition as a separate genus of Picornaviridae, and my role in the process. Poliovirus, the most hazardous of the group, is almost gone from the world, but the other enteroviruses will be with us for some time. Several members of the Committee dealing with these agents-Enders, Sabin, Dalldorf, Syverton-have passed on, but the work of this Committee to which I was privileged to contribute will live long. CONTENTS

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“…Despite that, the method is still time-consuming, labor-intensive, and costly; and the supply of antisera is limited. Moreover, there are frequent problems related to untypeable EVs that have been associated with mixtures of EVs, the existence of certain EV serotypes that cannot be identified with intersecting pools, the formation of aggregates (9), and the existence of antigenic variants of recognized EVs (13). Finally, some unrecognized serotypes are also untypeable by this standard method.…”

mentioning

confidence: 99%

Molecular Identification of Enterovirus by Analyzing a Partial VP1 Genomic Region with Different Methods

et al. 2002

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VP1 is the most suitable region for use in the identification of enterovirus. Although VP1 sequencing methods may vary, it is necessary to agree on a common strategy of sequence analysis. Identification of a strain type may be achieved by three different approaches: pairwise sequence alignment, multiple-sequence alignment, and phylogenetic inference. Other methods are also available, but they are not simple enough to be performed at a virology laboratory. best performances when used with the correct criteria. Other commercial and expensive programs did not achieve the same performances, making them less suitable for molecular typing of enteroviruses. Finally, although phylogenetic inference is the most demanding method in terms of knowledge of the user, it remained the best option analyzed.Enteroviruses (EVs) are a large genus belonging to the Picornaviridae family, and 64 immunologically distinct serotypes are known to cause infections in humans. They are the etiological agents responsible for several diseases (poliomyelitis, acute myocarditis, and aseptic meningitis) and play an important role in common chronic diseases, including dilated cardiomyopathy and insulin-dependent diabetes mellitus. Identification of EVs is essential for epidemiological surveillance, identification of polioviruses, the study of correlations between EV subtypes and diseases, identification of new EV types, and adequate treatment of EV infections in neonates and immunodeficient patients (for a complete review, see reference 15). Although the serotype identity can be determined by neutralization of infectivity with serotype-specific antisera, individual typing of all 64 serotypes by neutralization is clearly impractical. To overcome this problem, intersecting pools of individual antisera which allow the identification of a serotype were developed. Despite that, the method is still time-consuming, labor-intensive, and costly; and the supply of antisera is limited. Moreover, there are frequent problems related to untypeable EVs that have been associated with mixtures of EVs, the existence of certain EV serotypes that cannot be identified with intersecting pools, the formation of aggregates (9), and the existence of antigenic variants of recognized EVs (13). Finally, some unrecognized serotypes are also untypeable by this standard method.Because of these problems, several methods were developed for the molecular characterization of the genus (1,(5)(6)(7)17). The coupling of reverse transcription and amplification of the enteroviral RNA by PCR, followed by direct sequencing of the amplified products, is the general approach. Some of these methods analyzed the 5Ј noncoding, the VP2, or the 3D region of the EVs genome; but the sequence did not always correlate with the corresponding serotype (1, 10, 18). The VP1 region was the most suitable target due to the high correlation between serotypes and sequences and the availability of a large database of EVs sequences (17). Two methods were developed for the typing of EV strains by partial sequen...

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Improvement of Enterovirus Neutralization by Treatment with Sodium Deoxycholate or Chloroform

Cited by 29 publications

References 6 publications

Laboratory Diagnosis and Genetic Analysis of an Echovirus 30-Associated Outbreak of Aseptic Meningitis in Taiwan in 2001

Laboratory Diagnosis and Genetic Analysis of an Echovirus 30-Associated Outbreak of Aseptic Meningitis in Taiwan in 2001

My Role in the Discovery and Classification of the Enteroviruses

Molecular Identification of Enterovirus by Analyzing a Partial VP1 Genomic Region with Different Methods

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