Improvement of ethanol production using green alga<i>Chlorococcum minutum</i>

Varaprasad, Duddela; Narasimham, Dokka; Paramesh, K.; Sudha, Narravula Raga; Himabindu, Yeduguri; Kumari, Meruva Keerthi; Parveen, S. Nazaneen; Chandrasekhar, Thummala

doi:10.1080/09593330.2019.1669719

Cited by 12 publications

(9 citation statements)

References 36 publications

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“…In the present work, estimation of chlorophyll content was carried out using modified Arnon's [23] and Varaprasad et al [24] methods with 2-3 days old cultures. One gram of fresh algal material of both controls and treated samples were ground with a mortar and pestle using 80% acetone.…”

Section: Methodsmentioning

confidence: 99%

Effects of oxygen scavengers (Sodium sulfite, sodium bisulfite, sodium dithionite, and sodium metabisulfite) on growth and accumulation of biomass in the green alga Asterarcys quadricellulare

Sudha¹,

Varaprasad²,

Riazunnisa³

et al. 2022

J App Biol biotech

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The present investigation aims to know the effects of oxygen scavengers such as sodium sulfite (Na 2 SO 3 ), sodium bisulfite (NaHSO 3 ), sodium dithionite (Na 2 S 2 O 4 ), and sodium metabisulfite (Na 2 S 2 O 5 ) on growth and accumulation of biomass (chlorophylls) in green alga Asterarcys quadricellulare (A. quadricellulare). Alga is grown in tris-acetatephosphate (TAP) medium, along with various concentrations of NaHSO 3 , Na 2 SO 3 , Na 2 S 2 O 4 , and Na 2 S 2 O 5 individually under in vitro conditions. To evaluate the effects of scavengers, in vitro grown algal cells were used to estimate the chlorophyll a and b, along with total chlorophylls. Augmented growth and total chlorophyll content (63.23 mg/L) was noticed in A. quadricellulare cultures grown in TAP with 3.2 mM of sodium sulfite medium. TAP with 0.4 mM sodium bisulfite medium enhanced the growth and total chlorophyll content (49.38 mg/L) in this alga. Similarly, improved growth and total chlorophyll content (44.46 mg/L) was observed in A. quadricellulare grown in TAP with 0.4 mM of sodium dithionite medium. TAP with 0.08 mM sodium metabisulfite medium augmented the growth and total chlorophyll content (41.01 mg/L) in this alga. Higher doses of scavengers inhibit the growth and reduced the total chlorophyll contents in A. quadricellulare. The present work will be helpful to standardize the oxygen scavenger resistance levels in various algal species for anaerobic experiments including in vitro production of hydrogen.

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Section: Methodsmentioning

confidence: 99%

Effects of oxygen scavengers (Sodium sulfite, sodium bisulfite, sodium dithionite, and sodium metabisulfite) on growth and accumulation of biomass in the green alga Asterarcys quadricellulare

Sudha¹,

Varaprasad²,

Riazunnisa³

et al. 2022

J App Biol biotech

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“…Further, most of the municipal waste can also be used as feedstock for ethanol production [36]. Fermentation is a complex series of reactions that convert carbohydrates mainly sugars, starch, cellulose, hemicellulose and lignocellulosic materials into ethanol and CO 2 [18]. It works best at temperatures in the range of 25 • C-37 • C in the absence of oxygen and will produce aqueous solutions of upto 14% ethanol.…”

Section: Production Of Ethanolmentioning

confidence: 99%

“…Some studies have shown that temperatures above 37 °C are detrimental for ethanol production [65,72]. Our laboratory generated ethanol using the feedstocks of Chlamydomonas reinhardtii, Chlorella vulgaris and Chlorococcum minutum at 27 °C with Baker's yeast [11,13,18,24]. In addition, pH control is an important factor for algal and yeast or bacterial In general, algae have the capacity for self-fermentation under light and dark conditions.…”

Section: Effects Of Various Factors On Growth and Ethanol Production ...mentioning

confidence: 99%

“…Apart from energy usage, ethanol is used as a beverage and is also useful for the preparation of certain medicines and syrups. Therefore, bioethanol tends to provide a higher degree of national energy security in an eco-friendly and sustainable manner by adding conventional energy resources, decreasing dependence on imported fossil fuels and meeting energy needs [18]. With this background, this review intends to summarize the progress in ethanol production methods from algal species while keeping its excellent prospects in view.…”

Section: Introductionmentioning

confidence: 99%

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Algae: The Reservoir of Bioethanol

Chandrasekhar,

Varaprasad,

Gnaneswari

et al. 2023

Fermentation

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Overuse of non-renewable fossil fuels due to the population explosion urges us to focus on renewable fuels such as bioethanol. It is a well-known fact that ethanol is useful as a blending product with common fuels such as petrol and diesel. This reduces the cost besides bringing down environmental pollution. Apart from chemical methods, bioethanol is generated from photosynthetic plants including algae, plant-based products, microbial organisms and their waste. Specifically, the production of ethanol from microalgal sources has been an attractive method in recent days. The reason behind using microalgal species is their simple structure with photosynthetic ability. In contrast, certain algal species often go disused in some regions. Hence, the production of ethanol from algal sources is one of the best waste management practices. Moreover, it is easy to improve the biomass in microalgal species by altering the physicochemical conditions such as light, pH, temperature, external supply of nutrients, vitamins, nano-sized particles, gene alterations etc., which will enhance ethanol production. In this review, the methods used for ethanol production are discussed. In addition, the factors involved in algal growth and ethanol production are emphasized. Overall, this review focuses on ethanol production from various algal species. This information will be useful for industrial-level production of ethanol and future renewable energy research.

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“…Microalgae are photosynthetic microscopic organisms, either prokaryotic or eukaryotic that could live in all bodies of water and utilize sunlight and carbon dioxide (CO 2 ) as their sole energy and carbon sources to produce organic compounds via photosynthesis (Zhu et al, 2016;Deviram et al, 2020). This, together with their fast growth rates, and ability to produce high value metabolites of interest for many industrial applications, make microalgae an attractive subject for researchers in the field of sustainable production (Saadaoui et al, 2019;Arias et al, 2020;Rasheed et al, 2020;Saadaoui et al, 2019;Veiga et al, 2020;Saadaoui et al, 2019;Varaprasad et al, 2021;Bounnit et al, 2022;Bello et al, 2022). Furthermore, microalgae have also been found to be able to efficiently remove contaminants from various types of wastewater effluents, including pharmaceutical (Singh et al, 2020), agricultural (Leite et al, 2019), and industrial (Al-Jabri et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Genetic engineering to enhance microalgal-based produced water treatment with emphasis on CRISPR/Cas9: A review

Hassanien

Saadaoui

Schipper

et al. 2023

Front. Bioeng. Biotechnol.

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In recent years, the increased demand for and regional variability of available water resources, along with sustainable water supply planning, have driven interest in the reuse of produced water. Reusing produced water can provide important economic, social, and environmental benefits, particularly in water-scarce regions. Therefore, efficient wastewater treatment is a crucial step prior to reuse to meet the requirements for use within the oil and gas industry or by external users. Bioremediation using microalgae has received increased interest as a method for produced water treatment for removing not only major contaminants such as nitrogen and phosphorus, but also heavy metals and hydrocarbons. Some research publications reported nearly 100% removal of total hydrocarbons, total nitrogen, ammonium nitrogen, and iron when using microalgae to treat produced water. Enhancing microalgal removal efficiency as well as growth rate, in the presence of such relevant contaminants is of great interest to many industries to further optimize the process. One novel approach to further enhancing algal capabilities and phytoremediation of wastewater is genetic modification. A comprehensive description of using genetically engineered microalgae for wastewater bioremediation is discussed in this review. This article also reviews random and targeted mutations as a method to alter microalgal traits to produce strains capable of tolerating various stressors related to wastewater. Other methods of genetic engineering are discussed, with sympathy for CRISPR/Cas9 technology. This is accompanied by the opportunities, as well as the challenges of using genetically engineered microalgae for this purpose.

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Improvement of ethanol production using green algaChlorococcum minutum

Cited by 12 publications

References 36 publications

Effects of oxygen scavengers (Sodium sulfite, sodium bisulfite, sodium dithionite, and sodium metabisulfite) on growth and accumulation of biomass in the green alga Asterarcys quadricellulare

Effects of oxygen scavengers (Sodium sulfite, sodium bisulfite, sodium dithionite, and sodium metabisulfite) on growth and accumulation of biomass in the green alga Asterarcys quadricellulare

Algae: The Reservoir of Bioethanol

Genetic engineering to enhance microalgal-based produced water treatment with emphasis on CRISPR/Cas9: A review

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