Improvement of filtration performance of stirred ceramic membrane reactor and its application to rapid fermentation of lactic acid by dense cell culture of Lactococcus lactis

Kamoshita, Yuya; Ohashi, Ryo; Suzuki, Takahiro

doi:10.1016/s0922-338x(98)80087-1

Cited by 29 publications

(9 citation statements)

References 11 publications

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“…In addition, a number of research groups have focused their attention on improving LAB biomass production via fermentation by exploiting novel bioreactors that permit the exchange of the medium to prevent LA accumulation and therefore growth inhibition. The most interesting results have been achieved by Kamoshita et al (1998) that exploited a stirred ceramic membrane reactor to grow Lactococcus lactis yielding 140 g · L −1 of biomass. A bioreactor coupled to a microfiltration module was exploited for the production of an enzyme, superoxide dismutase from Streptococcus lactis giving a 4.3-fold increase in productivity (Taniguchi et al, 1989).…”

Section: Introductionmentioning

confidence: 99%

High cell density cultivation of probiotics and lactic acid production

Schiraldi

Adduci

Valli

et al. 2003

Biotech & Bioengineering

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The commercial interest in functional foods that contain live microorganisms, also named probiotics, is paralleled by the increasing scientific attention to their functionality in the digestive tract. This is especially true of yogurts that contain strains of lactic-acid bacteria of intestinal origin, among these, Lactobacillus delbrueckii ssp. bulgaricus is extensively used in the dairy industry and it has been demonstrated to be a probiotic strain. In this work we describe high cell density cultivations of this microorganism also focusing on the stereospecific production of lactic acid. Key parameters such as medium composition (bactocasitone concentration) and diverse aeration conditions were explored. The results showed that the final concentration of biomass in anaerobic fermentation was lower than the one obtained in microaerophilic conditions, while it gave a very high productivity of lactic acid which was present as a racemic mixture in the permeate. Fermentation experiments carried out with air sparging, even at very low flow-rate, led to the production of the sole L(+) lactic acid giving sevenfold increase in biomass yield in respect to the batch cultivation. Finally, a mathematical model was developed to describe the microfiltration bioprocess applied in this research considering an inhibition kinetic and enucleating a suitable mathematical description for the decrease of the transmembrane flux.

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Section: Introductionmentioning

confidence: 99%

High cell density cultivation of probiotics and lactic acid production

Schiraldi

Adduci

Valli

et al. 2003

Biotech & Bioengineering

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“…However, in comparison to organic membranes, ceramic membranes are more favorable for their integration in MBR systems on account of their high mechanical strength, thermal and chemical resistance, good cleanability, and long operation life, as membranes should be resistant to the high temperature and pressure and/or aggressive chemicals used in sterilization. (23,13). Besides LA production, microfiltration (MF), and ultrafiltration (UF) membranes were also successfully utilized in the field of water treatment (24,25), synthesis of food ingredients (26), production of emulsions (27), recovery of viral and virus-like particles (28)(29)(30)(31)(32), etc., and showed a great potential for process engineering.…”

Section: Introductionmentioning

confidence: 99%

Lactic acid production in a membrane bioreactor system with thermophilicBacillus coagulans: fouling analysis of the used ceramic membranes

Fan

Ebrahimi

Quitmann

et al. 2015

Separation Science and Technology

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In lactic acid fermentation broth systems, membrane fouling is characterized as the crucial factor in limiting ceramic membrane lifetime and efficiency. This study aimed to find the limiting factor of this process and seek an effective solution. By quantitative analysis using resistance-in-series model, the cake layer resistance is demonstrated to be the dominant factor in a steady state. A cake compressibility index was measured as high as 2.09. Within the low transmembrane pressure range, the cake resistance was manipulated through crossflow velocity regulation to achieve a better membrane performance. A new correlation between flux and cell density was also introduced.

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“…This set‐up filters large volumes without membrane clogging from retained cells. Since fouling during ISRP lowers filtration efficiency (Carstensen et al, ), different anti‐fouling concepts for individual bioreactors are currently being discussed in literature. Backflushing of fluids such as medium, filtrate, or air remove foulants from the membrane surface (Carstensen et al, ; Kamoshita et al, ; Park et al, ). Various backflushing techniques with different intervals and frequencies recover the product flux to its initial value (Carstensen et al, ; de Ven et al, ; Prip Beier and Jonsson, ; Suzuki et al, ).…”

Section: Introductionmentioning

confidence: 99%

A membrane stirrer for product recovery and substrate feeding

Femmer

Carstensen

Weßling

2014

Biotech & Bioengineering

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During fermentation processes, in situ product recovery (ISPR) using submerged membranes allows a continuous operation mode with effective product removal. Continuous recovery reduces product inhibition and organisms in the reactor are not exposed to changing reaction conditions. For an effective in situ product removal, submerged membrane systems should have a sufficient large membrane area and an anti-fouling concept integrated in a compact device for the limited space in a lab-scale bioreactor. We present a new membrane stirrer with integrated filtration membranes on the impeller blades as well as an integrated gassing concept in an all-in-one device. The stirrer is fabricated by rapid prototyping and is equipped with a commercial micromesh membrane. Filtration performance is tested using a yeast cell suspension with different stirring speeds and aeration fluxes. We reduce membrane fouling by backflushing through the membrane with the product stream.

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Improvement of filtration performance of stirred ceramic membrane reactor and its application to rapid fermentation of lactic acid by dense cell culture of Lactococcus lactis

Cited by 29 publications

References 11 publications

High cell density cultivation of probiotics and lactic acid production

High cell density cultivation of probiotics and lactic acid production

Lactic acid production in a membrane bioreactor system with thermophilicBacillus coagulans: fouling analysis of the used ceramic membranes

A membrane stirrer for product recovery and substrate feeding

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