Improvement of Flax Drought Tolerance Using Gene Transfer

Tawfik, Rasha Sabry; Badr, Abdelfattah; Sammour, R. H.; Ibrahim, Usama; Matter, Mohamed A.; Sakr, Mohamed

doi:10.3329/ptcb.v26i2.30570

Cited by 8 publications

(9 citation statements)

References 12 publications

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“…Since 1995, when long-term traditional breeding programs to enhance linseed stress tolerance and improve crop yield under periodic drought, transgenic linseed plants have been obtained for enhancing tolerance to DS [13,14]. Some transgenic linseed plants have been obtained for enhancing tolerance to drought stress [15]. Despite recent advances in linseed drought tolerance, how it functions is another open question PacBio's SMRT (single-molecule real-time) sequencing (PacBio, http://www.paci cbiosciences.com/)…”

Section: Introductionmentioning

confidence: 99%

Transcriptome analysis and molecular mechanism of linseed (Linum usitatissimum L.) drought tolerance under repeated drought using single-molecule long-read sequencing

Wang

Tan

et al. 2020

Preprint

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Background: Oil flax (linseed, Linum usitatissimum L.) is one of the most important oil crops., However, the increases in drought resulting from climate change have dramatically reduces linseed yield and quality, but very little is known about how linseed coordinates the expression of drought resistance gene in response to different level of drought stress (DS) on the genome-wide level.Results: To explore the linseed transcriptional response of DS and repeated drought (RD) stress, we determined the drought tolerance of different linseed varieties. Then we performed full-length transcriptome sequencing of drought-resistant variety (Z141) and drought-sensitive variety (NY-17) under DS and RD stress at the seedling stage using single-molecule real-time sequencing and RNA-sequencing. Gene Ontology (GO) and reduce and visualize GO (REVIGO) enrichment analysis showed that upregulated genes of Z141 were enriched in more functional pathways related to plant drought tolerance than those of NY-17 were under DS. In addition, 4436 linseed transcription factors were identified, and 1190 were responsive to stress treatments. Moreover, protein-protein interaction (PPI) network analysis showed that the proline biosynthesis pathway interacts with stress response genes through RAD50 (DNA repair protein 50) interacting protein 1 (RIN-1). Finally, proline biosynthesis and DNA repair structural gene expression patterns were verified by RT- PCR.Conclusions: The drought tolerance of Z141 may be related to its upregulation of drought tolerance genes under DS. Proline may play an important role in linseed drought tolerance by maintaining cell osmotic and protecting DNA from ROS damage. In summary, this study provides a new perspective to understand the drought adaptability of linseed.

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Section: Introductionmentioning

confidence: 99%

Transcriptome analysis and molecular mechanism of linseed (Linum usitatissimum L.) drought tolerance under repeated drought using single-molecule long-read sequencing

Wang

Tan

et al. 2020

Preprint

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“…Drought is an erratic and highly unpredictable environmental phenomenon; therefore, selection should target drought tolerant genotypes having yield potential. Accordingly, long-term traditional breeding programs and later development of transgenic flax were initiated to combat these constraints and improve flax production ( Tawfik et al, 2016 ). Since drought tolerance is a complex polygenic trait, understanding the adaptive mechanisms and identification of underlying genes/markers/QTLs could pave a way for genetic enhancement and productivity of flax in arid and semi-arid regions.…”

Section: Introductionmentioning

confidence: 99%

“…Another study reported overexpression of drought responsive element binding protein 2A (DREB2A) gene imparting drought tolerance in transgenic line of flax cv. Blanka(Tawfik et al, 2016). Similarly, for high temperature stress, few genes have been discovered and functionally characterized in flax.Saha et al (2019) reported the genome-wide identification of 34 putative HSF genes from the flax genome.…”

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confidence: 99%

Integrated omics approaches for flax improvement under abiotic and biotic stress: Current status and future prospects

et al. 2022

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Flax (Linum usitatissimum L.) or linseed is one of the important industrial crops grown all over the world for seed oil and fiber. Besides oil and fiber, flax offers a wide range of nutritional and therapeutic applications as a feed and food source owing to high amount of α-linolenic acid (omega-3 fatty acid), lignans, protein, minerals, and vitamins. Periodic losses caused by unpredictable environmental stresses such as drought, heat, salinity-alkalinity, and diseases pose a threat to meet the rising market demand. Furthermore, these abiotic and biotic stressors have a negative impact on biological diversity and quality of oil/fiber. Therefore, understanding the interaction of genetic and environmental factors in stress tolerance mechanism and identification of underlying genes for economically important traits is critical for flax improvement and sustainability. In recent technological era, numerous omics techniques such as genomics, transcriptomics, metabolomics, proteomics, phenomics, and ionomics have evolved. The advancements in sequencing technologies accelerated development of genomic resources which facilitated finer genetic mapping, quantitative trait loci (QTL) mapping, genome-wide association studies (GWAS), and genomic selection in major cereal and oilseed crops including flax. Extensive studies in the area of genomics and transcriptomics have been conducted post flax genome sequencing. Interestingly, research has been focused more for abiotic stresses tolerance compared to disease resistance in flax through transcriptomics, while the other areas of omics such as metabolomics, proteomics, ionomics, and phenomics are in the initial stages in flax and several key questions remain unanswered. Little has been explored in the integration of omic-scale data to explain complex genetic, physiological and biochemical basis of stress tolerance in flax. In this review, the current status of various omics approaches for elucidation of molecular pathways underlying abiotic and biotic stress tolerance in flax have been presented and the importance of integrated omics technologies in future research and breeding have been emphasized to ensure sustainable yield in challenging environments.

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Section: Introductionmentioning

confidence: 99%

Transcriptome analysis and molecular mechanism of linseed (Linum usitatissimum L.) drought tolerance under repeated drought using single-molecule long-read sequencing

Wang

Tan

et al. 2021

BMC Genomics

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Background Oil flax (linseed, Linum usitatissimum L.) is one of the most important oil crops., However, the increases in drought resulting from climate change have dramatically reduces linseed yield and quality, but very little is known about how linseed coordinates the expression of drought resistance gene in response to different level of drought stress (DS) on the genome-wide level. Results To explore the linseed transcriptional response of DS and repeated drought (RD) stress, we determined the drought tolerance of different linseed varieties. Then we performed full-length transcriptome sequencing of drought-resistant variety (Z141) and drought-sensitive variety (NY-17) under DS and RD stress at the seedling stage using single-molecule real-time sequencing and RNA-sequencing. Gene Ontology (GO) and reduce and visualize GO (REVIGO) enrichment analysis showed that upregulated genes of Z141 were enriched in more functional pathways related to plant drought tolerance than those of NY-17 were under DS. In addition, 4436 linseed transcription factors were identified, and 1190 were responsive to stress treatments. Moreover, protein-protein interaction (PPI) network analysis showed that the proline biosynthesis pathway interacts with stress response genes through RAD50 (DNA repair protein 50) interacting protein 1 (RIN-1). Finally, proline biosynthesis and DNA repair structural gene expression patterns were verified by RT- PCR. Conclusions The drought tolerance of Z141 may be related to its upregulation of drought tolerance genes under DS. Proline may play an important role in linseed drought tolerance by maintaining cell osmotic and protecting DNA from ROS damage. In summary, this study provides a new perspective to understand the drought adaptability of linseed.

show abstract

Improvement of Flax Drought Tolerance Using Gene Transfer

Cited by 8 publications

References 12 publications

Transcriptome analysis and molecular mechanism of linseed (Linum usitatissimum L.) drought tolerance under repeated drought using single-molecule long-read sequencing

Transcriptome analysis and molecular mechanism of linseed (Linum usitatissimum L.) drought tolerance under repeated drought using single-molecule long-read sequencing

Integrated omics approaches for flax improvement under abiotic and biotic stress: Current status and future prospects

Transcriptome analysis and molecular mechanism of linseed (Linum usitatissimum L.) drought tolerance under repeated drought using single-molecule long-read sequencing

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