Improvement of fungal lipids esterification process by bacterial lipase for biodiesel synthesis

Abd‐Alla, Mohamed Hemida; Bagy, M. M. K.; Morsy, Fatthy Mohamed; Hassan, Elhagag Ahmed

doi:10.1016/j.fuel.2015.07.080

Cited by 15 publications

(5 citation statements)

References 72 publications

(43 reference statements)

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“…Abd-Alla et al 76 reported that Bacillus vallismortis ASU3 (KP777551), Bacillus tequilensis ASU11 (KP777550), Bacillus amyloliquefaciens ASU16 (KP777549), and Bacillus firmus ASU32 (KP777552) exhibited the highest lipase production, recording 4.72 U/mL, 3.13 U/mL, 3.41 U/mL, and 4.28 U/mL, respectively. B. firmus ASU32 (KP777552) showed the highest activity toward the lipase transesterification processes of fungal lipids and displayed higher thermal stability and methanol tolerance, expecting their application as a promising biocatalyst for fatty acid methyl ester (FAME) synthesis.…”

Section: Resultsmentioning

confidence: 99%

“…9 ). Interestingly, Abd-Alla et al 76 reported that free or immobilized cells of lipase-producing Bacillus firmus showed a high conversion (78% FAME) for converting fungal lipids into biodiesel. Cervero et al 62 reached 48% biodiesel by use of the one-step methanol addition method during the lipase esterification process, whereas 90% of the biodiesel production was obtained through the use of the three-step addition method.…”

Section: Resultsmentioning

confidence: 99%

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Optimizing the catalytic activities of methanol and thermotolerant Kocuria flava lipases for biodiesel production from cooking oil wastes

Najjar

Hassan

Zabermawi

et al. 2021

Sci Rep

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In this study, two highly thermotolerant and methanol-tolerant lipase-producing bacteria were isolated from cooking oil and they exhibited a high number of catalytic lipase activities recording 18.65 ± 0.68 U/mL and 13.14 ± 0.03 U/mL, respectively. Bacterial isolates were identified according to phenotypic and genotypic 16S rRNA characterization as Kocuria flava ASU5 (MT919305) and Bacillus circulans ASU11 (MT919306). Lipases produced from Kocuria flava ASU5 showed the highest methanol tolerance, recording 98.4% relative activity as well as exhibited high thermostability and alkaline stability. Under the optimum conditions obtained from 3D plots of response surface methodology design, the Kocuria flava ASU5 biocatalyst exhibited an 83.08% yield of biodiesel at optimized reaction variables of, 60 ○C, pH value 8 and 1:2 oil/alcohol molar ratios in the reaction mixture. As well as, the obtained results showed the interactions of temperature/methanol were significant effects, whereas this was not noted in the case of temperature/pH and pH/methanol interactions. The obtained amount of biodiesel from cooking oil was 83.08%, which was analyzed by a GC/Ms profile. The produced biodiesel was confirmed by Fourier-transform infrared spectroscopy (FTIR) approaches showing an absorption band at 1743 cm−1, which is recognized for its absorption in the carbonyl group (C=O) which is characteristic of ester absorption. The energy content generated from biodiesel synthesized was estimated as 12,628.5 kJ/mol. Consequently, Kocuria flava MT919305 may provide promising thermostable, methanol-tolerant lipases, which may improve the economic feasibility and biotechnology of enzyme biocatalysis in the synthesis of value-added green chemicals.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Optimizing the catalytic activities of methanol and thermotolerant Kocuria flava lipases for biodiesel production from cooking oil wastes

Najjar

Hassan

Zabermawi

et al. 2021

Sci Rep

Self Cite

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“…Cheese whey supplemented with some nutrients is another precious substrate for bioenergy production . Adequate successes in utilizing renewable and cost‐effective waste materials as feedstocks have opened novel ground for the development of economic biofuel generation . In this study, OP could produce biofuel without any nutrient supplementation or use of a reducing agent.…”

Section: Resultsmentioning

confidence: 99%

“…86 Adequate successes in utilizing renewable and cost-effective waste materials as feedstocks have opened novel ground for the development of economic biofuel generation. [87][88][89] In this study, OP could produce biofuel without any nutrient supplementation or use of a reducing agent. The maximum biofuel production from OP in this study is acceptable when compared with values using other substrates reported in the literature (Table 4).…”

Section: Production Of Biofuel From Ops Bymentioning

confidence: 99%

Enhancement of biohydrogen production from sustainable orange peel wastes usingEnterobacterspecies isolated from domestic wastewater

et al. 2018

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Summary Five facultative anaerobic bacterial isolates were recovered from domestic wastewater. These isolates were identified based on the 16S rRNA as Enterobacter aerogenes (one isolate), Enterobacter cloacae (two isolates), and Cronobacter sakazakii (three isolates). These isolates were examined for their potential to evolve hydrogen on a glucose medium. The most potent hydrogen‐producing isolates, E aerogenes (KY549389) and E cloacae (KY524293), were examined for their capacity to generate hydrogen, acetone, butanol, and ethanol using orange peel (OP) hydrolysate. OP powder was pretreated with n‐hexane to remove the toxicity of d‐limonene. Different concentrations (4%, 6%, and 8% w/v) of limonene‐free OP were subjected to the boiling water (temperature of 100°C) or acid (HCl) treatments. The maximum fermentative H2 production of 1700 and 1620 mL/L was obtained from 6% OP hydrolysate extracted with boiling water using facultative anaerobic E aerogenes (KY549389) and E cloacae (KY524293), respectively. Hydrogen production efficiency was 0.99 and 1.19 mol H2/mol glucose for E aerogenes and E cloacae, respectively. The total fermentative acetone, butanol, and ethanol (ABE) generated by E aerogenes and E cloacae were 0.78 and 0.38 g/L including acetone (0.05 and 0.04 g/L), butanol (0.011 and 0.013 g/L), and ethanol (0.71 and 0.32 g/L), respectively. The maximum ABE productivity was 0.01 and 0.005 g/L/h generated at 60 g/L OP hydrolysate by E aerogenes and E cloacae, respectively. These strains were positive for nitrogen fixation (nitrogenase) capability estimated by the acetylene reduction assay. Application of OP hydrolysate without the addition of any nutritional components or reducing agent is considered an eco‐friendly, economical, and commercial substrate for desired biofuel production.

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“…Biodiesel is the esters of fatty acids generated from renewable sources such as edible/non-edible oils or animal fats by transesterification/esterification processes of triglycerides or free fatty acids [6,7]. Biodiesel is considered an excellent alternative fuel to conventional petroleum diesel owing to its biodegradability and nontoxicity [8,9]. In addition, the raw material is a critical consideration in the biodiesel industry because the cost of biodiesel is influenced mainly by the processed raw material utilized.…”

Section: Introductionmentioning

confidence: 99%

Biodiesel Production through Rhodotorula toruloides Lipids and Utilization of De-Oiled Biomass for Congo Red Removal

Almuhayawi,

Hassan,

Almasaudi

et al. 2023

Sustainability

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The current study aimed to investigate the potentiality of yeast isolate Rhodotorula toruloides Y1124 to be used as a feedstock for biodiesel production, and the reutilization of the de-oiled yeast biomass wastes as a biosorbent for the biosorption of Congo red from aquatic solutions was investigated. From screening results, eight yeast isolates were referred to as oleaginous microorganisms, of which yeast isolate Rhodotorula toruloides Y1124 was the highest lipid-accumulating isolate and was used as a feedstock for biodiesel production. The highest lipid accumulation (64.8%) was significantly dependent on the glucose concentration, pH, and incubation temperature according to Plackett–Burman and central composite design results. Under optimized conditions, the estimated amount of biodiesel synthesis from Rhodotorula toruloides biomass represented 82.12% of total analytes. The most prevalent fatty acid methyl esters were hexadecanoic and 11-octadecenoic, comprising 30.04 and 39.36% of total methyl esters which were compatible with plant oils. The optimum biosorption conditions for Congo red removal were pH 6, a 15 min contact time, and an initial dye concentration of 40 mg L−1. The biosorption isothermal and kinetics fitted well with the Langmuir model and the maximal biosorption capacity (qmax) was 81.697 mg g−1. Therefore, the current study may offer a sustainable feedstock with potential viability for both the synthesis of biodiesel and the removal of organic dyes.

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Improvement of fungal lipids esterification process by bacterial lipase for biodiesel synthesis

Cited by 15 publications

References 72 publications

Optimizing the catalytic activities of methanol and thermotolerant Kocuria flava lipases for biodiesel production from cooking oil wastes

Optimizing the catalytic activities of methanol and thermotolerant Kocuria flava lipases for biodiesel production from cooking oil wastes

Enhancement of biohydrogen production from sustainable orange peel wastes usingEnterobacterspecies isolated from domestic wastewater

Biodiesel Production through Rhodotorula toruloides Lipids and Utilization of De-Oiled Biomass for Congo Red Removal

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