Improvement of Gelatin Particle Agglutination Test for Detection of Anti‐HTLV‐I Antibody

Abstract

Partial modifications of antigen components were made to improve the gelatin particle agglutination (PA) test for the detection of antibodies against human T cell leukemia virus type‐I. Envelope glycoproteins prepared by lentil lectin affinity chromatography were further added to the purified viral antigens to be coated on the gelatin particles. Comparative studies with a conventional PA test kit (Serodia ATLA) and indirect immunofluorescence assay showed that the specificity and sensitivity of the new PA test were increased and that abnormal agglutination such as the prozone phenomenon was abolished by this improvement.

Association between Strongyloides stercoralis infection and biliary tract cancer

“…The controls included 1,458 patients aged ≥50 years, without any cancer or history of cancer, who were admitted to the Department of Medicine and Therapeutics, Ryukyu University Hospital, Okinawa, Japan, between 1991 and 2005. In all patients, serum anti-HTLV-1 antibody was measured by the gelatin particle agglutination method (Fujino et al 1991), infection of S. stercoralis was diagnosed using the agar plate culture method (Arakaki et al 1990), and liver flukes were diagnosed using the direct smear method. In 627 patients (115 patients with cancer and 512 patients without cancer), liver flukes were also diagnosed using the formalin-ether sedimentation method.…”

Monographs on the Evaluation of Carcinogenic Risks to Humans

“…The particle agglutination test uses gelatin paricles sensitized with HTL V -1 antigens Fujino et al, 1991).…”

Human sera from varicella-zoster virus (VZV) infections cross-react with human T cell leukaemia virus type 1 (HTLV-1): common epitopes in VZV gene 22 protein and HTLV-1 p19 gag protein

“…In this study we have demonstrated frequent positive reactions in the PA test for anti-HTLV-1 antibodies (Fujino et al, 1991) with sera derived from acute VZV infections. IgM antibodies are mostly responsible for this cross-reactivity.…”