Improvement of hemodynamics in mesenteric microcirculation in septic shock rats by anisodamine and anisodine

Zhong, Jianxin; Ouyang, Zhenhua; Shen, Jian; Zeng, Ye

doi:10.1016/j.mbm.2023.100006

Cited by 3 publications

(1 citation statement)

References 38 publications

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“…Both anisodamine hydrobromide (Ani HBr) and AT3 are atropine (ATP)-like agents and active ingredients derived from the roots of a Chinese specialty plant Scopolia tangutica maxim (Qiu et al, 2022;Zhong et al, 2023). AT3 was thought to be readily transported across the BBB, but Ani HBr was not, due to its structure, and exerts its effects mainly by improving the microcirculation function and scavenging the ROS (Chen et al, 2019;Liu et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Anisodine hydrobromide alleviates oxidative stress caused by hypoxia/reoxygenation in human cerebral microvascular endothelial cells predominantly via inhibition of muscarinic acetylcholine receptor 4

JIANG,

SHEN,

et al. 2023

BIOCELL

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Background: Anisodine hydrobromide (AT3), an anti-cholinergic agent, could be delivered to the brain across the blood-brain barrier and has been used clinically for the treatment of cerebral ischemia/reperfusion injury. Endothelial dysfunction can be caused by hypoxia/reoxygenation (H/R) via oxidative stress and metabolic alterations. The present study investigated whether AT3 regulates the production of nitric oxide (NO) and reactive oxygen species (ROS), and the HIF-1α pathway via regulation of muscarinic acetylcholine receptors (mAChRs) in brain microvascular endothelial cells after H/R exposure. Methods: Under H/R conditions, hCMEC/D3 cerebral microvascular endothelial cells were treated with AT3. Specific inhibitors of M2-and M4-mAChRs were used to explore the mechanism by which AT3 influences oxidative stress in endothelial cells. Then, mAChRs expression was detected by western blotting and NO production was detected by Greiss reaction. The intracellular ROS level was measured using DCFH-DA probes. The expression of hypoxia-inducible transcription factor 1α (HIF-1α) was also detected. Results: While H/R induced the expression of M2-and M4-mAChRs, AT3 suppressed the H/R-upregulated M2-and M4-mAChRs. H/R also induced the production of NO, ROS, and apoptosis. AT3 and M4-mAChR inhibitors inhibited the H/R-induced production of NO and ROS and apoptosis. HIF-1α was induced by H/R, but was suppressed by AT3. Conclusion: Thus, the in vitro evidence shows that AT3 protects against H/R injury in cerebral microvascular endothelial cells via inhibition of HIF-1α, NO and ROS, predominantly through the downregulation of M4-mAChR. The findings offer novel understandings regarding AT3-mediated attenuation of endothelial cell apoptosis and cerebral ischemia/ reperfusion injury.

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Section: Introductionmentioning

confidence: 99%

Anisodine hydrobromide alleviates oxidative stress caused by hypoxia/reoxygenation in human cerebral microvascular endothelial cells predominantly via inhibition of muscarinic acetylcholine receptor 4

JIANG,

SHEN,

et al. 2023

BIOCELL

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Comparative pharmacokinetic study of Anisodamine Hydrobromide tablets and injection in septic acute lung injury rats

Wu,

Zhang,

Liu

et al. 2024

Bioanalysis

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Anisodine hydrobromide injection in the treatment of acute ischemic stroke: A protocol for systematic review and meta-analysis

Liu,

Wang,

Wan

2023

Medicine: Case Reports and Study Protocols

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Background: Acute ischemic stroke (AIS) is a common disease of the central nervous system, with high mortality, and disability rates. Anisodine hydrobromide (Ani) injection has been used clinically for the treatment of AIS in China. However, no systematic review and meta-analysis have provided rigorous evidence for the clinical use of Ani injection in the treatment of AIS. This study aims to assess the efficacy and safety of Ani injection in the treatment of AIS. Methods: Following databases will be searched to identify eligible articles: CNKI, VIP, Wanfang, Chinese Biomedical Literature Database, EMBASE, PubMed, Cochrane, and Web of Science. All databases will be searched from their inception to May, 2023. RevMan software will be used for data analysis. Results: The results from meta-synthesis and descriptive analysis of National Institute of Health Stroke Scale, modified Rankin scale, Barthel Index, CT parameters, clinical efficacy rate, and adverse reactions of included articles will provide latest evidence. Conclusion: Findings of this study will provide a solid evidence-based basis for the rational and precise application of Ani injection for the treatment of AIS.

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Improvement of hemodynamics in mesenteric microcirculation in septic shock rats by anisodamine and anisodine

Cited by 3 publications

References 38 publications

Anisodine hydrobromide alleviates oxidative stress caused by hypoxia/reoxygenation in human cerebral microvascular endothelial cells predominantly via inhibition of muscarinic acetylcholine receptor 4

Anisodine hydrobromide alleviates oxidative stress caused by hypoxia/reoxygenation in human cerebral microvascular endothelial cells predominantly via inhibition of muscarinic acetylcholine receptor 4

Comparative pharmacokinetic study of Anisodamine Hydrobromide tablets and injection in septic acute lung injury rats

Anisodine hydrobromide injection in the treatment of acute ischemic stroke: A protocol for systematic review and meta-analysis

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