Improvement of hydrophilicity and cell attachment of polycaprolactone scaffolds using green synthesized carbon dots

Ehtesabi, Hamide; Massah, Fatemeh

doi:10.1016/j.mtsust.2021.100075

Cited by 43 publications

(23 citation statements)

References 38 publications

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“…This indicates that the higher number of cells on SF w/o pAsp films is the result of cell attachment rather than more proliferation on these films or more cell death in SF w/5% pAsp films. This is in contrast with literature reporting often a positive influence of hydrophilicity on cell attachment [38,39] . Interestingly, on mineralized SF w/o pAsp films, small particles in the proximity of cells were observed with SEM, which might indicate the presence of mineral nodules or matrix vesicles [40] (Figure 5J, white arrows).…”

Section: Resultscontrasting

confidence: 92%

Bioinspired Silk Fibroin Mineralization for Advanced In Vitro Bone Remodeling Models

Wildt

Bartels

Sommerdijk

et al. 2022

Preprint

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Human in vitro bone models can create the possibility for investigation of physiological bone remodeling while addressing the principle of replacement, reduction and refinement of animal experiments (3R). Current in vitro models lack cell-matrix interactions and their spatiotemporal complexity. To facilitate these analyses, a bone-mimetic template was developed in this study, inspired by bone extracellular matrix composition and organization. Silk fibroin (SF) was used as an organic matrix, poly-aspartic acid (pAsp) was used to mimic the functionality of non-collagenous proteins, and 10x simulated body fluid served as mineralization solution. By using pAsp in the mineralization solution, minerals were guided towards the SF material resulting in mineralization inside and as a coating on top of the SF. After cytocompatibility testing, remodeling experiments were performed in which mineralized scaffold remodeling by osteoclasts and osteoblasts was tracked with non-destructive micro-computed tomography and medium analyses over a period of 42 days. The mineralized scaffolds supported osteoclastic resorption and osteoblastic mineralization, in the physiological bone remodeling specific sequence. This model could therefore facilitate the investigation of cell-matrix interactions and may thus reduce animal experiments and advance in vitro drug testing for bone remodeling pathologies like osteoporosis, where cell-matrix interactions need to be targeted.

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Section: Resultscontrasting

confidence: 92%

Bioinspired Silk Fibroin Mineralization for Advanced In Vitro Bone Remodeling Models

Wildt

Bartels

Sommerdijk

et al. 2022

Preprint

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“…[19,20] 11) Can be synthesized to be highly hydrophilic or hydrophobic. [21,22] 12) Show potential for use as a multi-theragnostic platform (i.e., targeted drug/gene delivery + drug release through photothermal treatment + trackable in vivo with photoacoustic imaging). [23] 13) They can be integrated into other nano/micro composites for added functionality.…”

Section: Introductionmentioning

confidence: 99%

Carbon Dot Therapeutic Platforms: Administration, Distribution, Metabolism, Excretion, Toxicity, and Therapeutic Potential

Truskewycz

Yin

Halberg

et al. 2022

Small

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“…Additionally, reduced antimicrobial activity was observed for wound dressings [ 16 , 33 ] or increased C 2 C 12 proliferation for PLA surfaces [ 34 ]. Several studies have been devoted to enhancing the hydrophilicity of polymer surfaces to promote protein and cell attachment [ 35 , 36 , 37 , 38 ]. Moreover, plasma surface treatment was shown to directly affect biocompatibility since surface functionalization with NH 3 - or O 2 -plasma has been observed to improve cell growth [ 39 , 40 , 41 , 42 , 43 , 44 , 45 ].…”

Section: Introductionmentioning

confidence: 99%

Accelerated Endothelialization of Nanofibrous Scaffolds for Biomimetic Cardiovascular Implants

et al. 2022

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Nanofiber nonwovens are highly promising to serve as biomimetic scaffolds for pioneering cardiac implants such as drug-eluting stent systems or heart valve prosthetics. For successful implant integration, rapid and homogeneous endothelialization is of utmost importance as it forms a hemocompatible surface. This study aims at physicochemical and biological evaluation of various electrospun polymer scaffolds, made of FDA approved medical-grade plastics. Human endothelial cells (EA.hy926) were examined for cell attachment, morphology, viability, as well as actin and PECAM 1 expression. The appraisal of the untreated poly-L-lactide (PLLA L210), poly-ε-caprolactone (PCL) and polyamide-6 (PA-6) nonwovens shows that the hydrophilicity (water contact angle > 80°) and surface free energy (<60 mN/m) is mostly insufficient for rapid cell colonization. Therefore, modification of the surface tension of nonpolar polymer scaffolds by plasma energy was initiated, leading to more than 60% increased wettability and improved colonization. Additionally, NH3-plasma surface functionalization resulted in a more physiological localization of cell–cell contact markers, promoting endothelialization on all polymeric surfaces, while fiber diameter remained unaltered. Our data indicates that hydrophobic nonwovens are often insufficient to mimic the native extracellular matrix but also that they can be easily adapted by targeted post-processing steps such as plasma treatment. The results achieved increase the understanding of cell–implant interactions of nanostructured polymer-based biomaterial surfaces in blood contact while also advocating for plasma technology to increase the surface energy of nonpolar biostable, as well as biodegradable polymer scaffolds. Thus, we highlight the potential of plasma-activated electrospun polymer scaffolds for the development of advanced cardiac implants.

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Improvement of hydrophilicity and cell attachment of polycaprolactone scaffolds using green synthesized carbon dots

Cited by 43 publications

References 38 publications

Bioinspired Silk Fibroin Mineralization for Advanced In Vitro Bone Remodeling Models

Bioinspired Silk Fibroin Mineralization for Advanced In Vitro Bone Remodeling Models

Carbon Dot Therapeutic Platforms: Administration, Distribution, Metabolism, Excretion, Toxicity, and Therapeutic Potential

Accelerated Endothelialization of Nanofibrous Scaffolds for Biomimetic Cardiovascular Implants

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