Improvement of
            <i>R</i>
            ‐2‐(4‐hydroxyphenoxy) propionic acid biosynthesis of
            <i>Beauveria bassiana</i>
            by combined mutagenesis

Hu, Haifeng; Zhou, Haïyan; Cheng, Gao-Ping; Xue, Ya‐Ping; Wang, Yuan-Shan; Zheng, Yu‐Guo

doi:10.1002/bab.1872

Cited by 3 publications

(1 citation statement)

References 20 publications

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“…HPOPA and POPA concentrations were measured through HPLC analysis as described previously [ 30 ]. In order to measure the dry cell weight (DCW), fermentation broth was centrifuged at 12,000 g for 5 min.…”

Section: Methodsmentioning

confidence: 99%

Enhanced (R)-2-(4-Hydroxyphenoxy)Propionic Acid Production by Beauveria bassiana: Optimization of Culture Medium and H₂O₂ Supplement Under Static Cultivation

Zhou

Wang

et al. 2020

J. Microbiol. Biotechnol.

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( R )-2-(4-hydroxyphenoxy)propionic acid (HPOPA) is a key intermediate for the preparation of aryloxyphenoxypropionic acid herbicides ( R -isomer). In order to improve the HPOPA production from the substrate ( R )-2-phenoxypropionic acid (POPA) with Beauveria bassiana CCN-A7, static cultivation and H 2 O 2 addition were attempted and found to be conducive to the task at hand. This is the first report on HPOPA production under static cultivation and reactive oxygen species (ROS) induction. On this premise, the cultivation conditions and fermentation medium compositions were optimized. As a result, the optimal carbon source, organic nitrogen source, and inorganic nitrogen source were determined to be glucose, peptone, and ammonium sulfate, respectively. The optimal inoculum size and fermentation temperature were 13.3% and 28°C, respectively. The significant factors including glucose, peptone, and H 2 O 2 , identified based on Plackett-Burman design, were further optimized through Central Composite Design (CCD). The optimal concentrations were as follows: glucose 38.81 g/l, peptone 7.28 g/l, and H 2 O 2 1.08 g/l/100 ml. Under the optimized conditions, HPOPA titer was improved from 9.60 g/l to 19.53 g/l, representing an increase of 2.03- fold. The results obtained in this work will provide novel strategies for improving the biosynthesis of hydroxy aromatics.

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Section: Methodsmentioning

confidence: 99%

Enhanced (R)-2-(4-Hydroxyphenoxy)Propionic Acid Production by Beauveria bassiana: Optimization of Culture Medium and H₂O₂ Supplement Under Static Cultivation

Zhou

Wang

et al. 2020

J. Microbiol. Biotechnol.

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Revealing the regulatory impact of nutrient on the production of (R)-2-(4-Hydroxyphenoxy)propanoic acid by Beauveria bassiana biofilms through comparative transcriptomics analyse

Zou,

Ma,

Zhao

et al. 2024

Bioprocess Biosyst Eng

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Enhanced Production of Lipstatin Through NTG Treatment of Streptomyces toxytricini KD18 at 5 L Bioreactor Level

Khushboo

Dubey

2023

Appl Biochem Biotechnol

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Improvement of R ‐2‐(4‐hydroxyphenoxy) propionic acid biosynthesis of Beauveria bassiana by combined mutagenesis

Cited by 3 publications

References 20 publications

Enhanced (R)-2-(4-Hydroxyphenoxy)Propionic Acid Production by Beauveria bassiana: Optimization of Culture Medium and H₂O₂ Supplement Under Static Cultivation

Enhanced (R)-2-(4-Hydroxyphenoxy)Propionic Acid Production by Beauveria bassiana: Optimization of Culture Medium and H₂O₂ Supplement Under Static Cultivation

Revealing the regulatory impact of nutrient on the production of (R)-2-(4-Hydroxyphenoxy)propanoic acid by Beauveria bassiana biofilms through comparative transcriptomics analyse

Enhanced Production of Lipstatin Through NTG Treatment of Streptomyces toxytricini KD18 at 5 L Bioreactor Level

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Improvement of R ‐2‐(4‐hydroxyphenoxy) propionic acid biosynthesis of Beauveria bassiana by combined mutagenesis

Cited by 3 publications

References 20 publications

Enhanced (R)-2-(4-Hydroxyphenoxy)Propionic Acid Production by Beauveria bassiana: Optimization of Culture Medium and H2O2 Supplement Under Static Cultivation

Enhanced (R)-2-(4-Hydroxyphenoxy)Propionic Acid Production by Beauveria bassiana: Optimization of Culture Medium and H2O2 Supplement Under Static Cultivation

Revealing the regulatory impact of nutrient on the production of (R)-2-(4-Hydroxyphenoxy)propanoic acid by Beauveria bassiana biofilms through comparative transcriptomics analyse

Enhanced Production of Lipstatin Through NTG Treatment of Streptomyces toxytricini KD18 at 5 L Bioreactor Level

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Resources

About

Enhanced (R)-2-(4-Hydroxyphenoxy)Propionic Acid Production by Beauveria bassiana: Optimization of Culture Medium and H₂O₂ Supplement Under Static Cultivation

Enhanced (R)-2-(4-Hydroxyphenoxy)Propionic Acid Production by Beauveria bassiana: Optimization of Culture Medium and H₂O₂ Supplement Under Static Cultivation