Improvement of in vitro co-culture systems for bovine embryos using a low concentration of carbon dioxide and medium supplemented with β-mercaptoethanol

Geshi, Masaya; Yonai, Miharu; Sakaguchi, Minoru; Nagai, Takashi

doi:10.1016/s0093-691x(99)00009-6

Cited by 25 publications

(20 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Following cryopreservation, oocytes and embryos become especially more sensitive to the oxidative stress, resulting in lipid peroxidation, membrane injury and structural destruction [3,23,24]. Low molecular-weight thiol compounds, such as βME, maintain the redox state of cells and protect them against the harmful effects of oxidative injuries and a number of studies have indicated the promoting effects of βME during in vitro embryo production [14][15][16][17][18]. However, data on the effect of βME are conflicting or inconsistent regarding favorable concentration of βME used in culture medium, and regarding the stage of IVP in which βME supplementation better supports embryo development.…”

Section: Discussionmentioning

confidence: 99%

“…For example, Feugang et al [23] demonstrated no protective effect of βME on embryo development when added at 50 µM. In contrast, Geshi et al [16] showed that 10 µM βME in a co-culture system improves embryo development and Cammano et al [15] did not observed any difference between a low (10 µM) or high (100 µM) concentration of βME in development of resulting embryos. In addition, Geshi et al [16], Caamano et al [14] and Feuagang et al [23] reported better promoting effect of βME when added at 4-8 cell, 8-16 cell and beyond the morula stages, respectively.…”

Section: Discussionmentioning

confidence: 99%

“…To overcome this, exogenous antioxidants such as β-mercaptoethanol (βME), a low molecular weight thiol compound, have been frequently used to increase antioxidant capacity of embryos via increasing intracellular levels of reactive oxygen species (ROS) scavengers such as glutathione (GSH) [14][15][16][17][18]. Following cryopreservation, embryos become more amenable to the deleterious effects of ROS [2,4], and as a consequence, the importance of ROS detoxification seems to be greater than the normal sate of in vitro embryo development.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Antioxidant supplementation of culture medium during embryo development and/or after vitrification-warming; which is the most important?

Hosseini

Forouzanfar

Hajian

et al. 2009

J Assist Reprod Genet

View full text Add to dashboard Cite

Purpose To determine the most optimal stage for antioxidant supplementation of culture medium to improve developmental competence, cryotolerance and DNAfragmentation of bovine embryos. Methods Presumptive zygotes were first cultured in presence or absence of β-mercaptoethanol (β-ME), for 8 days. Subsequently, half of the expanded blastocysts developed in both groups were vitrified, warmed within 30 min and post-warming embryos along with their corresponding non-vitrified embryos were cultured for two further days in presence or absence of (100 µM) βME. Results For vitrified and non-vitrified embryos, the best effect was found when βME was added from day 1 of in vitro culture in continuation with post-warming culture period. Day 1-8 supplementation significantly increased the rates of cleavage, day 7 and day 8 blastocyst production. For non-vitrified embryos, βME addition during day 1-8 and/or 9-10 of embryo culture improved both hatching rate and quality of hatched embryos. For vitrified embryos, however, the percentage of DNA-fragmentation (18.5%) was significantly higher (p≤0.05) than that of embryos developed in absence of βME but supplemented with βME during post-warming period (13.5%). Conclusions Exogenous antioxidant increases the chance of embryos, even those of fair-quality, to develop to blastocyst. However, antioxidant inclusion during in vitro embryo development is not sufficient to maintain the redox state of these embryos during the critical period of post-warming embryo culture, and therefore, there should be a surplus source of exogenous antioxidant during post-warming embryo culture.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Antioxidant supplementation of culture medium during embryo development and/or after vitrification-warming; which is the most important?

Hosseini

Forouzanfar

Hajian

et al. 2009

J Assist Reprod Genet

View full text Add to dashboard Cite

show abstract

“…Historically, HEPES at 21 mM has been a standard for IVF handling media utilized for handling of gametes and embryos. Inclusion of HEPES at various concentrations from 2.5-25 mM in media used in elevated CO 2 concentrations has been used to mature oocytes [78], fertilize eggs [76,78] or culture embryos successfully [78][79][80], yielding rates similar to media with no HEPES present. Only when HEPES exceeded 35 mM in porcine embryo culture was any increased embryo fragmentation observed [79].…”

Section: Buffers In Ivfmentioning

confidence: 99%

Biological pH buffers in IVF: help or hindrance to success

Will

Clark

Swain

2011

J Assist Reprod Genet

View full text Add to dashboard Cite

Purpose Minimizing environmental stress helps maintain cellular homeostasis and is a crucial component in optimizing embryo development in vitro and resulting ART success. One stressor of particular interest is pH. Biologic buffers, such as HEPES and MOPS, are valuable tools for stabilizing pH. The objective of this manuscript is to summarize efficacy and impact of various pH buffers used during IVF lab procedures Methods Keyword searches were performed using Pubmed and Medline and relevant literature reviewed. Results Various pH buffers have been used with varying degrees of success for gamete and embryo processing in a variety of animal species, as well as in human. Conclusion Though biologic buffers off a means to improve pH stability, not all buffers may be appropriate for use with gametes and embryos. Specific buffers may have undesired effects, and these may be buffer, species, cell type or concentration dependent. Continued research is needed to further refine and improve the use of biologic buffers for use in human ART.

show abstract

“…b-mercaptoethanol is an useful and usual component in serum-free media for embryos culture rather than rCHO cell culture (Geshi et al 1999;Bagis and Mercan 2004) and chloroquine is famous for the therapeutic agent in the treatment of malaria (Ross et al 2000). Their unacquainted effects on rCHO cell growth and product formation offers interesting assignments, which are worth studying.…”

Section: Supplementation Of Hormones Inorganic Salts and Other Chemmentioning

confidence: 99%

Effects of Supplementation of Various Medium Components on Chinese Hamster Ovary Cell Cultures Producing Recombinant Antibody

et al. 2005

View full text Add to dashboard Cite

Thirteen vitamins, twenty amino acids, hormones, inorganic salts, and other chemical agents, which constitute typical serum-free media, were evaluated for the development of fortified medium to enhance cell growth and productivity of recombinant antibody in the cultures of the recombinant Chinese hamster ovary (rCHO) cells. Two different rCHO cell lines, rCHO-A producing recombinant antibodies against the human platelet and rCHO-B secreting recombinant antibodies against the S surface antigen of Hepatitis B, respectively, were cultivated in batch suspension mode. Concentration of interested component in the tested medium was doubled to examine the fortification effect. Growth of rCHO-A cell and its antibody production were slightly improved with addition of either choline chloride, folic acid, thiamineÁHCl, or Long TM R 3 IGF-I. On the other hand, in the cultivation of rCHO-B cell which was more sensitive to its environmental changes, hormones such as Long TM R 3 IGF-I and triiodothyronine (T 3 ) as well as various vitamins involving choline chloride, i-inositol, niacinamide, pyridoxineÁHCl, and thiamineÁHCl enhanced the cell growth and antibody production. Particularly, when concentration of consuming amino acid was doubled, remarkable increase in specific productivity was served, resulting in high final antibody concentration. These results were believed to provide a fundamental strategy of medium fortification useful for improvement of recombinant antibody production in serum-free medium.

show abstract

Improvement of in vitro co-culture systems for bovine embryos using a low concentration of carbon dioxide and medium supplemented with β-mercaptoethanol

Cited by 25 publications

References 22 publications

Antioxidant supplementation of culture medium during embryo development and/or after vitrification-warming; which is the most important?

Antioxidant supplementation of culture medium during embryo development and/or after vitrification-warming; which is the most important?

Biological pH buffers in IVF: help or hindrance to success

Effects of Supplementation of Various Medium Components on Chinese Hamster Ovary Cell Cultures Producing Recombinant Antibody

Contact Info

Product

Resources

About