Improvement of insulin resistance, blood pressure and interstitial pH in early developmental stage of insulin resistance in OLETF rats by intake of propolis extracts

Aoi, Wataru; Hosogi, Shigekuni; Niisato, Naomi; Yokoyama, Noriko; Hayata, Hiroki; Mitsuya, Hiroaki; Kusuzaki, Katsuyuki; Fukuda, Takuya; Fukui, Michiaki; Nakamura, Naoto; Marunaka, Yoshinori

doi:10.1016/j.bbrc.2013.02.029

Cited by 71 publications

(82 citation statements)

References 34 publications

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“…These results are also the indicator to mean that the exercise program applied to the experimental animals in this study was appropriately made in conjunction with the glycogen metabolism. However, the effect of the propolis intake could not be confirmed in this study, unlike in some previous studies that recently have argued the insulin sensitivity improvement [27] and blood glucose dropping effect related to the glucose metabolism [28,29] among the effects of physiological activity of propolis. In addition, it is considered that it should be taken account of the previous studies [32] that there may be the metabolic difference [30,31] according to the form of muscular fiber, or the difference of glycogen use according to fasting and the analysis timing after exercise loading for the fact that the rate of accumulation of glycogen in the tissue was relatively very high in the liver tissue in comparison with the skeletal muscle in this study.…”

Section: Discussioncontrasting

confidence: 99%

The effect of exercise training and water extract from propolis intake on the antioxidant enzymes activity of skeletal muscle and liver in rat

Kwon

Lee

Kim

2014

Journal of Exercise Nutrition and Biochemistry

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[Purpose]In this study, the authors have intended to investigate the effects that the exercise training and the intake of the water extract from propolis have on the activity of antioxidant enzymes.[Methods]For this purpose, the exercise training (70% VO2max treadmill running exercise for 60min)of 5 times per week for six weeks and the intake (50mg/kg/day) of the water extract from propolis were performed by separating the experimental animals (SD rats, n=32) into CON(n=8) group, CON+Ex(n=8), PA(n=8), and PA+Ex(n=8).[Results]As a result, the following conclusions were obtained: The concentration of the blood glucose and insulin of the CON+Ex group and PA+Ex group which are the exercise parallel group were significantly decreased in comparison with the control group, whereas if comparing the glycogen concentration in skeletal muscle and liver tissue between the exercise parallel group and the CON group, the former showed significantly high value in comparison with the latter (p < .05). In the case of the activity of the antioxidant enzyme in the skeletal muscle and the liver tissue, the activities of SOD, GPX and CAT in the gastrocnemius muscle tissue of the experimental animals showed significantly high value in PA+Ex group in comparison with other experimental groups (p < .05). In addition, the SOD activity in the liver tissue showed that only PA+Ex group was significantly increased, whereas GDX activity showed significantly higher value in CON+Ex group and PA group than CON group (p < .05). However, the activity of CAT in the liver tissue showed that there is no difference between the experimental groups. As a result that measured the concentration of MDA in order to evaluate the damage level of the tissue by oxygen free radicals, the difference between the groups in the liver tissue was not shown, while it was shown that only PA+Ex group in the skeletal muscle tissue was significantly decreased in comparison with other experimental groups (p < .05).[Conclusion]Taken together the above findings, it is considered that the parallel treatment of the exercise training and the water extract from propolis can not only increase the use of glycogen of the skeletal muscle and liver tissue, but also it can give the effect to suppress the creation of active oxygen by inducing the activity of the antioxidant enzyme in the body, and in the future, the possibility as the exercise supplements and the antioxidant of the water-soluble propolis are expected.

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Section: Discussioncontrasting

confidence: 99%

The effect of exercise training and water extract from propolis intake on the antioxidant enzymes activity of skeletal muscle and liver in rat

Kwon

Lee

Kim

2014

Journal of Exercise Nutrition and Biochemistry

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“…An ex vivo study by Yamauchi et al (24) reported that insulin-mediated 2-deoxy-D-glucose uptake by rat soleus muscle is inhibited by a reduction in the pH of the medium from 7.4 to 6.8. Furthermore, Aoi et al (25) have reported that a propolis-contained diet improved, by increasing, the pH of ascites and metabolic tissues compared to the normal diet and improved glucose metabolism in Otsuka Long-Evans Tokushima Fatty rats, whose pH in various tissues was lower compared to normal rats (25)(26)(27). Therefore, propolis may improve insulin sensitivity by an increase of extracellular pH.…”

Section: Placebo (N=39)mentioning

confidence: 99%

Effect of Brazilian green propolis in patients with type 2 diabetes: A double-blind randomized placebo-controlled study

et al. 2015

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Abstract.Propolis contains a variety of chemical compounds, including polyphenols, flavonoids, phenolic aldehydes, amino acids and vitamins, and presents numerous biological and pharmacological properties. The aim of the present study was to evaluate the effect of propolis on blood examination data in patients with type 2 diabetes. In the double-blind, 8-week randomized controlled study, 80 patients with type 2 diabetes were enrolled. Patients were randomly assigned to receive Brazilian green propolis (226.8 mg/day for 8 weeks) (n=41) or the placebo (n=39). The primary endpoint was to detect changes in blood examination data associated with metabolic disorders in patients suffering from diabetes mellitus, including the homeostasis model assessment of insulin resistance (HOMA-IR), uric acid and estimated glomerular filtration rate (eGFR) from baseline to the end of this study. The value of HOMA-IR was not significantly changed by the 8-week administration of propolis or placebo from the baseline data. Values of blood uric acid and eGFR in patients taking the placebo became worse at 8 weeks compared to the baseline, whereas this did not occur in patients consuming Brazilian green propolis. However, HOMA-IR was not improved by propolis intake. A randomized, controlled 8-week trial suggests that Brazilian green propolis (226.8 mg/day) prevents patients with type 2 diabetes from developing worse blood uric acid and eGFR.

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“…In peripheral blood of DM patient showed a significant increase of IFN-γ, TNF-α, and IL-8. These cytokines contribute to aggravate DM patient and in early stage also cause in the development of insulin resistance (Mahmoud and Al-Ozairi 2013; Aoi et al 2013). In the other hand propolis is known to contain high-level nutrient factor including vitamins, polyphenols, and amino acids that would be expected to improve insulin sensitivity.…”

Section: Introductionmentioning

confidence: 99%

Significance of propolis administration for homeostasis of CD4+CD25+ immunoregulatory T cells controlling hyperglycemia

Rifa’i

Widodo

2014

SpringerPlus

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In the present study, we examined the effect of ethanolic soluble derivative of propolis (EEP) extract on immunological function in diabetic mouse models with the aim of highlighting the role of regulatory T cell, the change of cell surface molecule, and in vivo productions of IFN-γ. Murine models of diabetes mellitus (DM) were created by injecting normal mice with S961 peptide. Normal BALB/c mice were injected intraperitoneally with peptide S961 300 mg/kg body weight (BW) twice a day for eight days. On day 15, the spleen was isolated; then, cell surface molecules and regulatory T cells were analyzed using flow cytometry. The histopathological changes were monitored in the liver of treated and control mice. Afterward, we tested the ability of propolis as an immunomodulator that initiate normality metabolism and homeostasis. The propolis decreased blood sugar levels and increased the number of naïve T cells expressing CD62L molecule by suppressing the development of effector cells in diabetic mice. However, the propolis stimulated development of effector cells, which was indicated by increasing the number of CD4+CD25+ T cells in normal mice. Moreover, the propolis increased the production of IFN-γ in normal mice, whereas in mouse models of diabetes mellitus propolis tends to suppress the production of IFN-γ. The histological analysis of the liver shows that at a dose of 50–200 mg/kg BW propolis does not show a toxic effect so that the dose is categorized safe. Therefore, the ethanolic soluble derivative of propolis (EEP) extract warrant for further exploited as an antidiabetic agent that safe for human.

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Improvement of insulin resistance, blood pressure and interstitial pH in early developmental stage of insulin resistance in OLETF rats by intake of propolis extracts

Cited by 71 publications

References 34 publications

The effect of exercise training and water extract from propolis intake on the antioxidant enzymes activity of skeletal muscle and liver in rat

The effect of exercise training and water extract from propolis intake on the antioxidant enzymes activity of skeletal muscle and liver in rat

Effect of Brazilian green propolis in patients with type 2 diabetes: A double-blind randomized placebo-controlled study

Significance of propolis administration for homeostasis of CD4+CD25+ immunoregulatory T cells controlling hyperglycemia

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