Improvement of Laccase Production in Pluerotus pulmonarius-LAU 09 by Mutation

Adebayo, Elijah Adegoke; Kola, Oloke Julius.; Yadav, A.; Chandral, Bora Tarun.

doi:10.5923/j.microbiology.20120201.03

Cited by 8 publications

(4 citation statements)

References 25 publications

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“…The increased yield in mannanase production in the mutants is a proof of true strain improvement. This was in agreement with works of Kang et al (1999) and Adegoke et al (2012), who reported an improvement in enzyme production in A. niger and Pluerotus pulmonarius-LAU through UV light mutation. Similarly, an improvement in cellulase production was reported by Shahbazi et al (2014) for UV mutants of Trichoderma reesei.…”

Section: Resultssupporting

confidence: 93%

UV Mutagenesis of Aspergillus flavus for Enhanced Mannanase Synthesis and Catabolite Activation Studies

Olaniyi¹,

Folasade²,

Familoni³

et al. 2015

Research J. of Microbiology

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The present investigation was conducted to generate catabolite activation mutants of Aspergillus flavus through UV mutagenesis. Mutants of A. flavus were generated by exposure of spores suspension to UV irradiation at a distance of 13 cm in dark from the centre of germicidal lamp (240 nm). Quantitatively, mannanase activity was determined using dinitrosalicylic acid method, while protein content was determined by Lowry method. Mannanase production by the mutants varied with time of exposure to UV irradiation. All the mutants except for mutant designated AFUV90 showed higher specific mannanase activity in comparison with the parent strain. The isolated mutants were screened for catabolite activation studies in the presence of different mannose and glycerol concentrations (1 and 1% w/v) as carbon sources. The supplementation of 0.1 and 1% (w/v) mannose in the fermentation media caused activation of mannanase biosynthesis in 100 and approximately 91% of the mutants, respectively. The inclusion of 0.1 and 1% (w/v) glycerol induced an improvement in approximately 82 and 55% of the mutants, respectively in terms of mannanase biosynthesis. The generation of catabolite activation mutants through UV mutagenesis might be considered as a break through in the industrial production of mannanase.

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Section: Resultssupporting

confidence: 93%

UV Mutagenesis of Aspergillus flavus for Enhanced Mannanase Synthesis and Catabolite Activation Studies

Olaniyi¹,

Folasade²,

Familoni³

et al. 2015

Research J. of Microbiology

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“…The supernatant was discarded, and pellet was kept at room temperature till the residual acetone evaporated. It was then dissolved in 0.1 M Tris–HCl buffer (pH 8.0) (Adegoke et al 2012 ).…”

Section: Methodsmentioning

confidence: 99%

An extracellular thermo-alkali-stable laccase from Bacillus tequilensis SN4, with a potential to biobleach softwood pulp

et al. 2014

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Degradation of residual lignin in kraft pulp by chemical bleaching is implicated in causing environmental pollution. The use of thermo- and alkali-tolerant bacterial laccases is considered to be important biological alternative to chemical processing. Laccases from Bacillus species have shown promise in this respect but their intracellular/spore bound presence make their industrial application economically unfeasible. We report here on a novel extracellular active thermo-alkali-stable laccase (SN4 laccase) which is active at 90 °C and pH 8.0 using 2,6-dimethoxyphenol as substrate from Bacillus tequilensis SN4. SN4 laccase retained 27 % activity for 5 min at 100 °C and more than 80 % activity for 24 h at 70 °C. The enzyme is also stable at a higher pH (9.0–10.0). Enzyme production was optimized by submerged fermentation. Relatively high yields (18,356 nkats ml−1) of SN4 laccase was obtained in a medium containing 650 μM MnSO4, 350 μM FeSO4, and 3.5 % ethanol. A 764-fold increase in laccase activity was observed under optimal conditions. In addition, reduction in kappa number and increase in brightness of softwood pulp by 28 and 7.6 %, respectively, were observed after treatment with SN4 laccase without a mediator. When N-hydroxybenzotriazole was used as a mediator, the kappa number was decreased to 47 % and brightness was increased to 12 %.

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“…This culture was incubated at 25 ± 2°C for 70 hours and subsequently re-introduced onto the newly prepared PDA slant, incubated for 2 weeks to obtain fully ramified UV10 strain. The wild and UV10 strains were re-evaluated for quality enhancement and used for the production of EPS using combined methods of Adebayo et al [33] and Majolagbe et al [34].…”

Section: Induction Of Physical Mutationmentioning

confidence: 99%

Biogenic Synthesis of Silver Nanoparticle from Mushroom Exopolysaccharides and its Potentials in Water Purification

Adeeyo¹,

Odiyo²

2018

CHEM

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Objective:This study reports a novel eco-friendly biosynthesis of Silver Nanoparticles (AgNPs) from Exopolysaccharides (EPS) ofLentinus edodesafter an attempt to optimise the production of EPS through mutagenesis. It further describes some potential application of silver nanoparticles in water treatment.Methods:A wild strain ofL. edodeswas subjected to UV irradiation, a physical mutagen, at 254 nm. The wild and resultant irradiated strains were then assessed for the production of EPS and subsequent application of the crude EPSs for biosynthesis of AgNPs. The particles were characterised by colour pattern and UV-visible spectroscopy. Based on superior EPS production and nanoparticle attributes, nanoparticles obtained from UV irradiated process were further subjected to Scanning Electron Microscopy (SEM). EPS produced was quantified by the phenol-sulphuric acid method and studied by GC-MS.Results:Results obtained for EPS productivity indicated the presence of monomer sugars such as arabinose (50.65%), mannose (19.20%), mannitol (15.58%), fructose (7.96%), trehalose (6.49%), and glucuronic acid, xylose, galactose and glucose with low percentages of ≤ 0.11. EPS productivity of wild and mutant strains was obtained as 1.044 and 2.783 mg/ml, respectively, after 7 days of fermentation. The result of EPS production for UV irradiated strain corresponds to a yield improvement of 2.7 fold of the wild-type. UV Spectroscopy and SEM analysis studies on EPS nanoparticle product of the improved (UV irradiated) strain indicated the formation of AgNPs at the absorption band of 421 nm with a size range of 50-100 nm.Conclusion:This study, which aimed at eco-friendly synthesis of myco-nanoparticle has established the novel ability ofL. edodes’polysaccharide in silver nanoparticles biosynthesis. It expounded potential frontiers of silver nanoparticles application in the water industry. To the best of the authors’ knowledge, this result represents the first report on the biosynthesis of AgNPs usingL. edode’sEPS.

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Improvement of Laccase Production in Pluerotus pulmonarius-LAU 09 by Mutation

Cited by 8 publications

References 25 publications

UV Mutagenesis of Aspergillus flavus for Enhanced Mannanase Synthesis and Catabolite Activation Studies

UV Mutagenesis of Aspergillus flavus for Enhanced Mannanase Synthesis and Catabolite Activation Studies

An extracellular thermo-alkali-stable laccase from Bacillus tequilensis SN4, with a potential to biobleach softwood pulp

Biogenic Synthesis of Silver Nanoparticle from Mushroom Exopolysaccharides and its Potentials in Water Purification

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