Improvement of lipid content of Chlorella minutissima MCC 5 for biodiesel production

Chakraborty, Sunandan; Mohanty, D. K.; Ghosh, Supratim; Das, Debabrata

doi:10.1016/j.jbiosc.2016.01.015

Cited by 36 publications

(15 citation statements)

References 53 publications

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“…The observed growth patterns under N and P-limiting medium (Fig. 1) are consistent with those reported for other Chlorella strains [17,14,25]. N-0.75, N-limiting condition; N-2.25, N-replete medium; P-0.02, P-limiting condition and P-0.06, Preplete medium; Control, BG-11 containing N-1.5 g L −1 + P-0.04 g L −1 .…”

Section: Cell Growth and Biomass Accumulationsupporting

confidence: 89%

“…The observed overall increase in biomass yield and biomass productivity in N and P rich medium of this strain may be due to luxurious uptake of phosphorus which gets deposited in the cell as polyphosphate involved in metabolic pathway and storage for further use during phosphorus starvation/ limitation [30]. Similarly, [25] reported an increase in biomass concentration of Chlorella minutissima MCC as the phosphate concentration increase from 0 to 3 mM.…”

Section: Cell Growth and Biomass Accumulationmentioning

confidence: 59%

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Lipid Productivity and Biosynthesis Genes Response of Indigenous Chlorella sp. T4 Strain under Different Nitrogen and Phosphorus Load

Gumbi

Kumar

Olaniran

2021

Preprint

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Microalgae can synthesize and accumulate high neutral lipids upon exposure to abiotic stress such as nutrient starvation or limitation. In this study, indigenous microalgae Chlorella sp. T4 was cultivated in nitrogen and phosphorus under both limiting and replete conditions. Growth, lipid yield, fatty acid profiles and biosynthetic gene expression levels were determined to ascertain cell’s response under these conditions. An impaired cell growth was observed under nitrogen limiting condition, evident by the lowest biomass yield (0.58±0.03 g L−1) as revealed by low quantum efficiency of photosystem II (Fv/Fm) value and chlorophyll a content. An increase in lipid content yield was observed under nitrogen and phosphorus limiting conditions as compared to the control. Nutrient limiting conditions produced fatty acid methyl ester that is suitable for biodiesel production compared to the control (BG-11). Gene expression analysis using real time q-PCR for photosynthesis (rbcL) and lipid biosynthesis (accD, KAS-1, ω-6 FAD, ω-3 FAD) genes revealed different expression levels under both limiting and replete conditions. Under nutrient limiting conditions, increase in the expression of accD, KAS-1, ω-6 FAD and ω-3 FAD genes was observed, whereas a decrease in rbcL gene expression level was noted. A significant correlation could be drawn between the expression levels of the biosynthetic genes and growth rate, biomass yield, physiological response, lipid yield and fatty acid composition. These results provide an insight into the physiological response and gene expression level under different nutrient levels, which could be harnessed for future genetic engineering of Chlorella sp. T4 for improved lipid production.

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Section: Cell Growth and Biomass Accumulationsupporting

confidence: 89%

Section: Cell Growth and Biomass Accumulationmentioning

confidence: 59%

Lipid Productivity and Biosynthesis Genes Response of Indigenous Chlorella sp. T4 Strain under Different Nitrogen and Phosphorus Load

Gumbi

Kumar

Olaniran

2021

Preprint

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“…Abou-Shady's design selection is a saturated (three-level) non-linear Taguchi-type (L9(3 4 )) orthogonal array [7]. The unreplicated-saturated L9(3 4 ) OA has been featured as a preferred trial planner in nonlinear screening/optimization in diverse areas of studies that involve complex chemometrics [56][57][58][59][60][61][62][63][64][65][66][67][68][69][70].…”

Section: Methodology Methodology Methodology Methodology 21 a Brief Description Of The Wastewater Electrodialysis Experimentsmentioning

confidence: 99%

Concurrent multiresponse multifactorial screening of an electrodialysis process of polluted wastewater using robust non-linear Taguchi profiling

Besseris

2020

Chemometrics and Intelligent Laboratory Systems

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This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

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“…Yustinadiar et al, 2020), temperatures (Chakraborty et al, 2016), and optimal nutrients used in the medium (Chandra et al, 2019). In the future, this microbial consortium combined with optimal bioreactor could be considered for the new method for culturing microalgae on the industrial scale that has the potency for biodiesel at a low cost and environment friendly (Allen et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Exploration of IAA Producing Bacteria And Amylolitic Bacteria From Several East Java Lakes, and Their Potency For Microbial Consortium To Accelerate Chlorella Vulgaris Growth

Nafi'ah

Prabaningtyas

Witjoro

et al. 2021

Preprint

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The consortium of various types of bacteria from lakes in East Java has the potency to stimulate microalgae Chlorella vulgaris growth. Increased microalgae density from co-culture has an excellent potency for sources of biomass that can be developed for renewable energy. Several stages conducted of this research started from an exploration of IAA producing bacteria and amylolytic bacteria from several East Java Lakes; then, the highest bacterial isolates were identified with morphological and genotypical characteristics. The well-characterized bacterial isolates were used for the microbial consortium in co-culture with C. vulgaris. The treatment used in this study as follows: I) C. vulgaris without bacteria culture as a control, II) amylolytic bacteria + C. vulgaris, III) IAA-producing bacteria + C. vulgaris, IV) potential amylolytic bacteria and IAA-producing bacteria + C. vulgaris. The exploration result of potential bacteria from Ranu Pani, Ranu Regulo, Telaga Ngebel, and Ranu Grati lakes was found 53 amylolytic bacterial isolates, and 90 isolates IAA-producing bacteria. The highest amylolytic bacteria (isolate L) is related to Bacillus amyloliquefaciens, while the most elevated IAA-producing bacteria (isolate C) is related to Bacillus paramycoides. The highest cell density was produced in treatment III, reaching 2.7 x 106 cells/mL on day 50th. The treatments with supplement bacteria showed a significant effect for accelerating the growth of microalgae compared to control.

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Improvement of lipid content of Chlorella minutissima MCC 5 for biodiesel production

Cited by 36 publications

References 53 publications

Lipid Productivity and Biosynthesis Genes Response of Indigenous Chlorella sp. T4 Strain under Different Nitrogen and Phosphorus Load

Lipid Productivity and Biosynthesis Genes Response of Indigenous Chlorella sp. T4 Strain under Different Nitrogen and Phosphorus Load

Concurrent multiresponse multifactorial screening of an electrodialysis process of polluted wastewater using robust non-linear Taguchi profiling

Exploration of IAA Producing Bacteria And Amylolitic Bacteria From Several East Java Lakes, and Their Potency For Microbial Consortium To Accelerate Chlorella Vulgaris Growth

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