Improvement of lipid production from an oil-producing filamentous fungus, Penicillium brevicompactum NRC 829, through central composite statistical design

Ali, Thanaa H.; El-Gamal, Mamdouh S.; El-Ghonemy, Dina H.; Awad, Ghada E. A.; Tantawy, Amir E.

doi:10.1007/s13213-017-1287-x

Cited by 18 publications

(7 citation statements)

References 35 publications

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“…According to this figure the lipid production content is increased when the incubating time is increased up to about 170 h. According to LINGO software, the optimum time required for the fungal strain growth is about 168 h (7 days). These results are in agreement with data obtained by Ali et al (2017), who reported that the maximum lipid production by Aspergillus spp. was obtained after 5 days of incubation.…”

Section: Effect Of Incubation Time On Lipid Productionsupporting

confidence: 94%

Optimization of cultural conditions for lipid accumulation by Aspergillus wentii Ras101 and its transesterification to biodiesel: application of response surface methodology

et al. 2018

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The present study is aimed to maximize biodiesel production by using the fungal strain Ras101 as a feedstock. Response surface methodology was used to relate the interaction between some nutritional and environmental factors affecting the lipid productivity by Ras101. By applying LINGO optimization program, the maximum lipid production of 40% dry biomass of this fungal isolate has been attained in a fermentation medium composed of 50 g/l glucose, 1 g/l nitrates, 1.5 g/l phosphorous, and 0.5 g/l NaCl. This medium was adjusted at pH of 6, and incubated at 28 °C for 7 days. The values of correlation errors between the experimental and estimated values are less than 1%; this proves that the proposed correlation could be used effectively for estimating the fungal lipid production. Consequently, the effects of time and temperature on the amount of biodiesel produced in the extraction and transesterification one-step process have been investigated. The maximum biodiesel production of 28% dry biomass (80% lipid) has been achieved in the transesterification process at 70 °C for 30 min. Additionally, it is found that the combination of glucose, nitrogen and phosphorous contents has a positive influence on lipid production in the fungal biomass. The density, kinematic viscosity, water content and calorific value of the produced biodiesel were 800 kg/m, 2.8 mm/s, 66 ppm and 10122 kcal/kg, respectively that matched well with biodiesel and fossil standard specifications.

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Section: Effect Of Incubation Time On Lipid Productionsupporting

confidence: 94%

Optimization of cultural conditions for lipid accumulation by Aspergillus wentii Ras101 and its transesterification to biodiesel: application of response surface methodology

et al. 2018

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“…Other prominent lipid-producing fungi that are well studied and characterised include the Penicillium sp. [54], Fusarium sp. [55], Mortierella sp.…”

Section: Oleaginous Fungimentioning

confidence: 99%

Prospect of metabolic engineering in enhanced microbial lipid production: review

Saha

Mukhopadhyay²

2021

Biomass Conv. Bioref.

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The use of fossil fuels has increasingly become associated with negative influences like detrimental environmental effects. This has made renewables, especially biofuels like biodiesel, a highly attractive substitute for energy generation. The industrial and economic potential of biodiesel-a lipid-based fuel-has increased interest and research into oleaginous microorganisms to synthesise and produce lipids. Effective identification and characterisation of a vast array of oleaginous microorganisms have led them to be employed for their efficient lipogenesis capability and ability to use a wide range of synthetic and non-expensive substrates. However, low lipid production has limited the use of microbially sourced lipids for biodiesel production at the industrial level. Nevertheless, the improvement and engineering of robust strains of these microbes through metabolic engineering have increased their lipid production capacity, leading to commercialising the lipids. This review provides a comprehensive outlook into the identification of different oleaginous microorganisms and their unique characteristics, which makes them highly valuable. An insight into the lipid biosynthesis pathways is provided and the role that several enzymes and regulators play in the metabolism of lipid accumulation. A detailed outlook is provided on the broad range of metabolic engineering approaches with regard to enhanced lipid production in several oleaginous microorganisms.

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“…Similarly, Abdelhamid (2018) reported a maximum lipid accumulation of 64.59% lipid/dry biomass from Fusarium oxysporum NRC 2017 at medium pH 5. Likewise, Ali et al, (2017) reported that the pH medium of 5.0 was optimal for lipid production by Penicillium brevicompactum Complex physiological parameters such as cell morphology and membrane permeability can be determined by the pH of the growth medium, as any variation in the pH of broth medium will greatly affect the membrane osmosis to absorb or move certain ions in the surrounding medium.…”

Section: Effect Of Ph Incubation Period and Temperature On Lipid Productionmentioning

confidence: 99%

Enhancement of lipid productivity from a promising oleaginous fungus <em>Aspergillus</em> sp. strain EM2018 for biodiesel production: Optimization of culture conditions and identification

et al. 2020

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Oleaginous fungi have recently gained increasing attention among different microorganisms due to their ability for lipid production for the preparation of biofuel. In the present study, a locally isolated fungus E45, identified genetically as Aspergillus sp. strain EM2018, was found to produce 25.2% of the total lipids content of its dry cell weight (DCW). Optimization of culture conditions was performed and lipid accumulation increased by about 2.4 fold (from 25.2% to 60.1% of DCW) when the fungus was grown for seven days in the potato dextrose (50 g/L) liquid medium at pH 5.0, incubation temperature at 30 ºC and inoculum size of 2 × 106 spore/mL. Supplementation of the medium with yeast extract and NaNO3 at a concentration of 0.05% as organic and inorganic nitrogen sources, respectively, increased lipid production (53.3% lipid/dry biomass). Gas chromatography analysis of fungal lipids revealed the presence of saturated (mainly palmitic acid C16:0 (33%) and lignoceric acid C24:0 (15%)) and unsaturated fatty acids in different proportions (mainly linoleic acid C18:2 (24.4%), oleica cid C18:1 (14%) and arachidonic C20:4 (7.4%). These findings suggest this new oleaginous fungus as a promising feedstock for various industrial applications and for the preparation of biodiesel.

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Improvement of lipid production from an oil-producing filamentous fungus, Penicillium brevicompactum NRC 829, through central composite statistical design

Cited by 18 publications

References 35 publications

Optimization of cultural conditions for lipid accumulation by Aspergillus wentii Ras101 and its transesterification to biodiesel: application of response surface methodology

Optimization of cultural conditions for lipid accumulation by Aspergillus wentii Ras101 and its transesterification to biodiesel: application of response surface methodology

Prospect of metabolic engineering in enhanced microbial lipid production: review

Enhancement of lipid productivity from a promising oleaginous fungus <em>Aspergillus</em> sp. strain EM2018 for biodiesel production: Optimization of culture conditions and identification

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