Improvement of Oxidative Stress-induced Cytotoxicity of Angelica keiskei (Miq.) Koidz. Leaves Extract through Activation of Heme Oxygenase-1 in C2C12 Murine Myoblasts

Park, Cheol; Kim, Da Hye; Kim, Tae Hee; Jeong, Seong Un; Yoon, Jae‐Hyun; Moon, Sung‐Kwon; Kwon, Chan-Young; Park, Shin‐Hyung; Hong, Su Hyun; Shim, Jung-Hyun; Kim, Gi‐Young; Choi, Yung Hyun

doi:10.1007/s12257-022-0310-7

Cited by 5 publications

(3 citation statements)

References 47 publications

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“…This is consistent with the results shown here. In most cells, including C2C12 cells, the reduction in Bcl-2/Bax ratio caused by H 2 O 2 was linked to the PARP cleavage by activated caspase-3, but many natural products with antioxidant activity appear able to reverse this ( You et al ., 2021 ; Park et al ., 2023 ). Therefore, caspase activation and PARP degradation can be used as representative biomarkers of apoptotic induction ( Wen et al ., 2012 ; Srivastava and Saxena, 2023 ), and the suppressive effect of morroniside on H 2 O 2 -mediated apoptosis of C2C12 cells appears responsible for their blockade.…”

Section: Discussionmentioning

confidence: 99%

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Morroniside Protects C2C12 Myoblasts from Oxidative Damage Caused by ROS-Mediated Mitochondrial Damage and Induction of Endoplasmic Reticulum Stress

Hwangbo,

Park,

Bang

et al. 2024

Biomol Ther (Seoul)

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Oxidative stress contributes to the onset of chronic diseases in various organs, including muscles. Morroniside, a type of iridoid glycoside contained in Cornus officinalis , is reported to have advantages as a natural compound that prevents various diseases. However, the question of whether this phytochemical exerts any inhibitory effect against oxidative stress in muscle cells has not been well reported. Therefore, the current study aimed to evaluate whether morroniside can protect against oxidative damage induced by hydrogen peroxide (H 2 O 2 ) in murine C2C12 myoblasts. Our results demonstrate that morroniside pretreatment was able to inhibit cytotoxicity while suppressing H 2 O 2 -induced DNA damage and apoptosis. Morroniside also significantly improved the antioxidant capacity in H 2 O 2 -challenged C2C12 cells by blocking the production of cellular reactive oxygen species and mitochondrial superoxide and increasing glutathione production. In addition, H 2 O 2 -induced mitochondrial damage and endoplasmic reticulum (ER) stress were effectively attenuated by morroniside pretreatment, inhibiting cytoplasmic leakage of cytochrome c and expression of ER stress-related proteins. Furthermore, morroniside neutralized H 2 O 2 -mediated calcium (Ca 2+ ) overload in mitochondria and mitigated the expression of calpains, cytosolic Ca 2+ -dependent proteases. Collectively, these findings demonstrate that morroniside protected against mitochondrial impairment and Ca 2+ -mediated ER stress by minimizing oxidative stress, thereby inhibiting H 2 O 2 -induced cytotoxicity in C2C12 myoblasts.

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Section: Discussionmentioning

confidence: 99%

“…No. CRL-1772™) were maintained in at 37°C under an atmosphere with 5% CO 2 as described in previous studies ( Park et al ., 2023 ). Supplies needed for cell culture were purchased from WelGENE, Inc. (Gyeongsan, Korea).…”

Section: Methodsmentioning

confidence: 99%

Morroniside Protects C2C12 Myoblasts from Oxidative Damage Caused by ROS-Mediated Mitochondrial Damage and Induction of Endoplasmic Reticulum Stress

Hwangbo,

Park,

Bang

et al. 2024

Biomol Ther (Seoul)

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“…Chalcones, arguably the most abundant bioactive components of the plant, are a class of unsaturated aromatic ketones, with over 20 forms identified, the most potent and abundant being 4-hydroxyderricin and xanthoangelol [ 9 ]. Chalcones are recognized for their antibacterial, antifungal, antiviral, antitumor, antidiabetic, and anti-inflammatory properties, mediated by their action on nitric oxide regulation and macrophage stimulation [ 2 , 10 , 11 , 12 , 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

Angelica keiskei Impacts the Lifespan and Healthspan of Drosophila melanogaster in a Sex and Strain-Dependent Manner

et al. 2023

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Angelica keiskei is a perennial plant, belonging to the Apiaceae family and originating from Japan. This plant has been reported to act as a diuretic, analeptic, antidiabetic, hypertensive, tumor, galactagogue, and laxative. The mechanism of action of A. keiskei is not known, but previous studies have suggested that it may act as an antioxidant. In this work, we used Drosophila melanogaster to evaluate the impact of A. keiskei on lifespan and healthspan and its potential anti-aging mechanism by conducting multiple assays on three fly strains: w1118, chico, and JIV. We observed that the extract extended lifespan and improved healthspan in a sex- and strain-dependent manner. A. keiskei extended lifespan and improved reproductive fitness in female flies and either had no effect or decreased survival and physical performance in males. The extract protected against the superoxide generator paraquat in both sexes. These sex-specific effects suggest that A. keiskei may act through age-specific pathways such as the insulin and insulin-like growth factor signaling (IIS) pathways. Upon examination, we found that the increased survival of A. keiskei-fed females was dependent on the presence of the insulin receptor substrate chico, supporting the role of IIS in the action of A. keiskei.

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Hesperidin protects C2C12 myoblasts from oxidative damage by reducing ROS-mediated mitochondrial damage and endoplasmic reticulum stress

Choi

2024

Mol. Cell. Toxicol.

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Improvement of Oxidative Stress-induced Cytotoxicity of Angelica keiskei (Miq.) Koidz. Leaves Extract through Activation of Heme Oxygenase-1 in C2C12 Murine Myoblasts

Cited by 5 publications

References 47 publications

Morroniside Protects C2C12 Myoblasts from Oxidative Damage Caused by ROS-Mediated Mitochondrial Damage and Induction of Endoplasmic Reticulum Stress

Morroniside Protects C2C12 Myoblasts from Oxidative Damage Caused by ROS-Mediated Mitochondrial Damage and Induction of Endoplasmic Reticulum Stress

Angelica keiskei Impacts the Lifespan and Healthspan of Drosophila melanogaster in a Sex and Strain-Dependent Manner

Hesperidin protects C2C12 myoblasts from oxidative damage by reducing ROS-mediated mitochondrial damage and endoplasmic reticulum stress

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