Improvement of pectinase, xylanase and cellulase activities by ultrasound: Effects on enzymes and substrates, kinetics and thermodynamic parameters

Dalagnol, Luíza M.G.; Silveira, Vitória C.C.; Silva, Halisson Baron da; Manfroi, Vítor; Rodrigues, Rafael C.

doi:10.1016/j.procbio.2017.06.029

Cited by 58 publications

(37 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Ultrasound is a potential method for modifying enzyme activity (Delgado‐Povedano & Castro, ; Huang, Chen, et al., ; Nadar & Rathod, ), and many researchers have used it to improve food enzymes. For example, Dalagnol, Silveira, Silva, Manfroi, and Rodrigues () found that ultrasound improved the activities of pectinase, xylanase, and cellulase. Ma et al.…”

Section: Introductionmentioning

confidence: 99%

Effects of power ultrasound on the activity and structure of β‐D‐glucosidase with potentially aroma‐enhancing capability

Sun

Zeng

Xue

et al. 2019

Food Science & Nutrition

View full text Add to dashboard Cite

β‐ d ‐glucosidase can release aroma precursors to improve the flavor of plant food, but the hydrolysis efficiency of the enzyme is low; the purpose of this study was to improve the enzyme activity using ultrasound. The effects of ultrasound parameters on β‐ d ‐glucosidase activity were investigated, and the respective structures of enzyme activated and enzyme inhibited were further analyzed. Low temperature (20–45°C), low ultrasonic intensity (<181.53 W/cm 2 ), and short treatment time (<15 min) led to the activation of β‐ d ‐glucosidase, whereas high temperature (45–60°C), high ultrasonic intensity (>181.53 W/cm 2 ), and long treatment time (>15 min) led to its inhibition. Application of ultrasound lowered the optimum temperature for β‐ d ‐glucosidase activity from 50 to 40°C. Ultrasound did not change the primary structures of the enzyme, but changed the secondary structures. When ultrasound activated β‐ d ‐glucosidase, the α‐helix contents were increased, the β‐fold and irregular coil content were reduced. When ultrasound inhibited β‐ d ‐glucosidase, the contents of β‐folds were increased, the α‐helix and irregular coil contents were reduced.. In summary, activation or inhibition of β‐ d ‐glucosidase under ultrasound was determined by the ultrasound conditions. This study suggests that ultrasound combined with β‐D‐glucosidase can be used in aroma‐enhancing.

show abstract

Section: Introductionmentioning

confidence: 99%

Effects of power ultrasound on the activity and structure of β‐D‐glucosidase with potentially aroma‐enhancing capability

Sun

Zeng

Xue

et al. 2019

Food Science & Nutrition

View full text Add to dashboard Cite

show abstract

“…For both biocatalysts under study, the variations in enthalpy and entropy were approximately twice bigger for the ultrasonic irradiation system, when compared to the orbital shaking one. The variation in enthalpy depends on the structure of the enzyme and its capacity of formation and disruption of chemical bonds [94], whereas the variation in entropy is associated with the number of molecules of the substrates with sufficient energy to react [95,96]. In fact, at 37 kHz, 300 W and for the range of temperature under study (30 to 45 °C), the ultrasonic irradiation has favored the synthesis of ethyl butyrate biocatalyzed by immobilized lipases.…”

Section: Resultsmentioning

confidence: 99%

Ethyl Butyrate Synthesis Catalyzed by Lipases A and B from Candida antarctica Immobilized onto Magnetic Nanoparticles. Improvement of Biocatalysts’ Performance under Ultrasonic Irradiation

Monteiro

Neto

Fechine

et al. 2019

IJMS

View full text Add to dashboard Cite

The synthesis of ethyl butyrate catalyzed by lipases A (CALA) or B (CALB) from Candida antarctica immobilized onto magnetic nanoparticles (MNP), CALA-MNP and CALB-MNP, respectively, is hereby reported. MNPs were prepared by co-precipitation, functionalized with 3-aminopropyltriethoxysilane, activated with glutaraldehyde, and then used as support to immobilize either CALA or CALB (immobilization yield: 100 ± 1.2% and 57.6 ± 3.8%; biocatalysts activities: 198.3 ± 2.7 Up-NPB/g and 52.9 ± 1.7 Up-NPB/g for CALA-MNP and CALB-MNP, respectively). X-ray diffraction and Raman spectroscopy analysis indicated the production of a magnetic nanomaterial with a diameter of 13.0 nm, whereas Fourier-transform infrared spectroscopy indicated functionalization, activation and enzyme immobilization. To determine the optimum conditions for the synthesis, a four-variable Central Composite Design (CCD) (biocatalyst content, molar ratio, temperature and time) was performed. Under optimized conditions (1:1, 45 °C and 6 h), it was possible to achieve 99.2 ± 0.3% of conversion for CALA-MNP (10 mg) and 97.5 ± 0.8% for CALB-MNP (12.5 mg), which retained approximately 80% of their activity after 10 consecutive cycles of esterification. Under ultrasonic irradiation, similar conversions were achieved but at 4 h of incubation, demonstrating the efficiency of ultrasound technology in the enzymatic synthesis of esters.

show abstract

“…Proper intensity ultrasonic waves can enhance the dissolution of substrate (Bochu et al, 2003) thus the catalysis effect of enzymes improves (Delgado-Povedano and De Castro, 2015). Dalagnol et al (2017) reported a 17% increase in the catalytic efficiency of cellulase with sonication possibly by increasing the reaction stability. In an earlier study, Gadalkar and Rathod (2017) observed an increase of 1.2 fold in the yield of ferulic acid with ultrasound assisted enzymatic pretreatment.…”

Section: Discussionmentioning

confidence: 99%

The Effect of Ultrasound Processing and Exogenous Cellulase Supplementation on the Ruminal Degradability of Palm Date Seeds

Aboragah¹,

Embaby²,

Günal³

et al. 2019

American Journal of Animal and Veterinary Sciences

View full text Add to dashboard Cite

The objective of this study was to evaluate the effects of sonication and enzyme supplements on rumen degradability of date palm seeds (DPS). The effect of alkali-assisted sonication and the addition of cellulase enzyme supplements (40 endoglucanase units/g dry matter) derived from Aspergillus niger or Trichoderma longibrachiatum on DPS ruminal degradability were evaluated under in vitro conditions. The in vitro ruminal degradability of neutral detergent fiber (NDF) and organic matter (OM) for the unsonicated seeds was not affected (p>0.10) by enzyme supplements. On the other hand, alkali-assisted sonication increased the in vitro rumen degradability of seeds' NDF and OM and the addition of Trichoderma longibrachiatum cellulase enzyme to sonicated seeds slightly but significantly increased (p<0.01) their in vitro NDF and OM degradability. The results indicate that sonication along with alkali pretreatment could improve the NDF and OM degradability of DPS in rumen. The pretreatment combined effect of ultrasound and Trichoderma longibrachiatum cellulase enzyme could be beneficial in increasing the inclusion rate of DPS in ruminant animals' diets.

show abstract

Improvement of pectinase, xylanase and cellulase activities by ultrasound: Effects on enzymes and substrates, kinetics and thermodynamic parameters

Cited by 58 publications

References 41 publications

Effects of power ultrasound on the activity and structure of β‐D‐glucosidase with potentially aroma‐enhancing capability

Effects of power ultrasound on the activity and structure of β‐D‐glucosidase with potentially aroma‐enhancing capability

Ethyl Butyrate Synthesis Catalyzed by Lipases A and B from Candida antarctica Immobilized onto Magnetic Nanoparticles. Improvement of Biocatalysts’ Performance under Ultrasonic Irradiation

The Effect of Ultrasound Processing and Exogenous Cellulase Supplementation on the Ruminal Degradability of Palm Date Seeds

Contact Info

Product

Resources

About