Improvement of phytochemical production by plant cells and organ culture and by genetic engineering

Nielsen, Erik; Temporiti, Marta Elisabetta Eleonora; Cella, Rino

doi:10.1007/s00299-019-02415-z

Cited by 61 publications

(31 citation statements)

References 134 publications

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“…Plants can synthesize many bioactive molecules, better known as “phytochemicals,” which aid in the protection of plants, and can have a huge impact on human health and metabolism [1]. A wide variety of medicinal properties are exhibited by different plants ranging from anti-inflammatory, antioxidant, antitumor and immunomodulatory effects [2], apart from improving cardiovascular ailments [3], treating kidney stones [4] and digestive diseases like inflammatory bowel disease [5], and many more.…”

Section: Introductionmentioning

confidence: 99%

Antiaging, Stress Resistance, and Neuroprotective Efficacies ofCleistocalyx nervosumvar.panialaFruit Extracts UsingCaenorhabditis elegansModel

Prasanth

Brimson

Chuchawankul

et al. 2019

Oxidative Medicine and Cellular Longevity

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Plant parts and their bioactive compounds are widely used by mankind for their health benefits. Cleistocalyx nervosum var. paniala is one berry fruit, native to Thailand, known to exhibit various health benefits in vitro. The present study was focused on analyzing the antiaging, stress resistance, and neuroprotective effects of C. nervosum in model system Caenorhabditis elegans using physiological assays, fluorescent imaging, and qPCR analysis. The results suggest that the fruit extract was able to significantly extend the median and maximum lifespan of the nematode. It could also extend the healthspan by reducing the accumulation of the “age pigment” lipofuscin, inside the nematode along with regulating the expression of col-19, egl-8, egl-30, dgk-1, and goa-1 genes. Further, the extracts upregulated the expression of daf-16 while downregulating the expression of daf-2 and age-1 in wild-type nematodes. Interestingly, it could extend the lifespan in DAF-16 mutants suggesting that the extension of lifespan and healthspan was dependent and independent of DAF-16-mediated pathway. The fruit extract was also observed to reduce the level of Reactive Oxygen Species (ROS) inside the nematode during oxidative stress. The qPCR analysis suggests the involvement of skn-1 and sir-2.1 in initiating stress resistance by activating the antioxidant mechanism. Additionally, the fruit could also elicit neuroprotection as it could extend the median and maximum lifespan of transgenic strain integrated with Aβ. SKN-1 could play a pivotal role in establishing the antiaging, stress resistance, and neuroprotective effect of C. nervosum. Overall, C. nervosum can be used as a nutraceutical in the food industry which could offer potential health benefits.

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Section: Introductionmentioning

confidence: 99%

Antiaging, Stress Resistance, and Neuroprotective Efficacies ofCleistocalyx nervosumvar.panialaFruit Extracts UsingCaenorhabditis elegansModel

Prasanth

Brimson

Chuchawankul

et al. 2019

Oxidative Medicine and Cellular Longevity

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“…Indeed, SA elicitor application at the highest concentration dramatically stressed the saffron cell culture, causing cell death within 24 h. In this time, the elicitor also increased the production of saffron natural metabolites especially crocin, and caused their exudation into the culture medium. This behavior makes SA a promising elicitor to be used in a possible scaling-up procedure for bioreactor production of saffron bioactive compounds [41,42].…”

Section: Discussionmentioning

confidence: 99%

Salycilic Acid Induces Exudation of Crocin and Phenolics in Saffron Suspension-Cultured Cells

Moradi

Zarinkamar

Domenico

et al. 2020

Plants

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The production of crocin, an uncommon and valuable apocarotenoid with strong biological activity, was obtained in a cell suspension culture of saffron (Crocus sativus L.) established from style-derived calli to obtain an in-vitro system for metabolite production. Salycilic acid (SA) was used at different concentrations to elicit metabolite production, and its effect was analyzed after a 4 days of treatment. HPLC-DAD analysis was used for total crocin quantification while the Folin-Ciocâlteu method was applied for phenolic compounds (PC) content. Interestingly, despite cell growth inhibition, a considerable exudation was observed when the highest SA concentration was applied, leading to a 7-fold enhanced production of crocin and a 4-fold increase of phenolics compared to mock cells. The maximum antioxidant activity of cell extracts was evidenced after SA 0.1 mM elicitation. Water-soluble extracts of saffron cells at concentrations of 1, 0.5, and 0.1 µg mL−1 showed significant inhibitory effects on MDA-MB-231 cancer cell viability. The heterologous vacuolar markers RFP-SYP51, GFPgl133Chi, and AleuRFP, were transiently expressed in protoplasts derived from the saffron cell suspensions, revealing that SA application caused a rapid stress effect, leading to cell death. Cell suspension elicitation with SA on the 7th day of the cell growth cycle and 24 h harvest time was optimized to exploit these cells for the highest increase of metabolite production in saffron cells.

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“…These indicated that the developed protocol, which was initiated under lower BA, suitable ICD, and appropriate aeration rate, supported the best condition for the cells to grow and develop. Cells in liquid culture would be in full contact with the culture medium and with continuous aeration, the medium would stayed in homogeneity and enable to facilitate efficient nutrient uptake (Wang et al, 2019) and continuously supplies of oxygen leading to its faster growth (Fernandes & Cabral, 2016;Nielsen, Temporiti, & Cella, 2019).…”

Section: Ec Proliferation In An Air-lift Bioreactor Culturementioning

confidence: 99%

Rapid Somatic Embryogenesis of Citrus reticulata Blanco cv. Madu in an Air-Lift Bioreactor Culture

Agisimanto¹,

Normah

Ibrahim

2019

Agrivita.J.Agr.Sci

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Embryogenic cell (EC) growth and proliferation as well as somatic embryo induction were examined in a bioreactor culture using Murashige and Skoog basal medium particularly for the influence of 6-benzyladenine (BA) concentration, initial cell density and aeration rate. Embryogenic callus was induced from seeds of Citrus reticulata Blanco cv. Madu. The cell suspension in a 3-L bioreactor exhibited maximum cell growth following the addition of 1.5 mg/l of BA. The fresh weight (FW) of the cells after 28 days of growth was found to increase from an initial of 5.5 g cell culture to 57.3 g, a 10.4-fold increase and the maximum growth rate (GR) of the cells (0.33 g/day) was attained by the 7 th day of culture. In the cell density experiment, ECs at a concentration of 5.5 g/l constituted the most effective inoculum, reaching the highest GR of ECs (0.52 g/ day), again by the 7 th day of culture. In the aeration experiment, the highest EC GR of 2.6 g/day was obtained at the maximum aeration rate of 1.5 vvm (air volume medium/ volume/min). After 28 days of somatic embryogenesis, 79% of ECs became somatic embryos, of which 29% were at cotyledonary stage.

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Improvement of phytochemical production by plant cells and organ culture and by genetic engineering

Cited by 61 publications

References 134 publications

Antiaging, Stress Resistance, and Neuroprotective Efficacies ofCleistocalyx nervosumvar.panialaFruit Extracts UsingCaenorhabditis elegansModel

Antiaging, Stress Resistance, and Neuroprotective Efficacies ofCleistocalyx nervosumvar.panialaFruit Extracts UsingCaenorhabditis elegansModel

Salycilic Acid Induces Exudation of Crocin and Phenolics in Saffron Suspension-Cultured Cells

Rapid Somatic Embryogenesis of Citrus reticulata Blanco cv. Madu in an Air-Lift Bioreactor Culture

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