Improvement of rifamycin B production using mutant strains of Amycolatopsis mediterranei

Venkateswarlu, G.; Murali, Poornimaa; Sharma, Gita; Rao, L. Venkateswar

doi:10.1007/s004499900068

Cited by 9 publications

(9 citation statements)

References 13 publications

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“…Complete knowledge of optimal conditions required for maximum fermentation activity leading to antimicrobial metabolite production by actinomycetes is required so as to standardize the different physical and physiological factors influencing the production of metabolites with antibiotic properties with particular reference to the strain used [6]. The present article deals with isolation of actinomycete strains from the marine soil samples collected from Palk Strait, East Coast of India and characterization of potent strains capable of synthesizing novel antimicrobial compounds, determination of the superior strain at species level and optimization of conditions required for the production of antimicrobial compound.…”

Section: Introductionmentioning

confidence: 99%

Optimization of Antimicrobial Production by a Marine Actinomycete Streptomyces afghaniensis VPTS3-1 Isolated from Palk Strait, East Coast of India

et al. 2011

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Totally 25 marine soil samples were collected from the region of Palk Strait of Bay of Bengal, Tamil Nadu, and were subjected to the isolation of actinomycetes. Sixty-eight morphologically distinct isolates were obtained and 37% (25) of them had antimicrobial activity. The potential producer was named as Streptomyces sp. VPTS3-1 and the phylogenetic evaluation on the basis of 16S rDNA sequence further categorized the organism as Streptomyces afghaniensis VPTS3-1. Further, the antimicrobial compound was extracted from the isolate using various solvents and the antimicrobial efficacies were tested against bacterial and fungal pathogens. In addition, in vitro optimization of parameters for the antimicrobial compound production revealed that the suitable pH as 7-8, the period of incubation as 9 days, temperature (30°C), salinity (2%), and starch and KNO 3 as the suitable carbon and nitrogen sources respectively in starch-casein medium.

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Section: Introductionmentioning

confidence: 99%

Optimization of Antimicrobial Production by a Marine Actinomycete Streptomyces afghaniensis VPTS3-1 Isolated from Palk Strait, East Coast of India

et al. 2011

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“…The reported yields of Rifamycin B are 2.45 g L À1 in 12 days with barbital using Amycolatopsis mediterranei VA18 strain (Venkateswarlu et al, 2000) and 2.20 g L À1 without bar- bital using Amycolatopsis mediterranei ATCC 21789 strain (Richard and Lancini, 1975). We used the Amycolatopsis mediterranei S699 strain that does not require barbital (Yu et al, 2001).…”

Section: Resultsmentioning

confidence: 97%

“…The parameter range for the medium component was selected based on previous reports (Kim et al, 1996;Richard and Lancini, 1975;Sensi and Thiemann, 1967;Venkateswarlu et al, 2000) as follows:…”

Section: Medium Componentsmentioning

confidence: 99%

Optimization of rifamycin B fermentation in shake flasks via a machine‐learning‐based approach

Bapat

Wangikar

2004

Biotech & Bioengineering

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Rifamycin B is an important polyketide antibiotic used in the treatment of tuberculosis and leprosy. We present results on medium optimization for Rifamycin B production via a barbital insensitive mutant strain of Amycolatopsis mediterranei S699. Machine-learning approaches such as Genetic algorithm (GA), Neighborhood analysis (NA) and Decision Tree technique (DT) were explored for optimizing the medium composition. Genetic algorithm was applied as a global search algorithm while NA was used for a guided local search and to develop medium predictors. The fermentation medium for Rifamycin B consisted of nine components. A large number of distinct medium compositions are possible by variation of concentration of each component. This presents a large combinatorial search space. Optimization was achieved within five generations via GA as well as NA. These five generations consisted of 178 shake-flask experiments, which is a small fraction of the search space. We detected multiple optima in the form of 11 distinct medium combinations. These medium combinations provided over 600% improvement in Rifamycin B productivity. Genetic algorithm performed better in optimizing fermentation medium as compared to NA. The Decision Tree technique revealed the media-media interactions qualitatively in the form of sets of rules for medium composition that give high as well as low productivity.

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“…Scaling up and optimization of antibiotic production in the fermentor necessitates studying influences of different physical and physiological factors affecting its production; taking into concern that the optimal fermentation conditions are related to the strain used [9]. Fed-batch addition of 5% glucose syrup at day 3 or day 4 gives almost the same yield (17-17.5 g/l) and even further addition of 5% glucose syrup at day 7 decreased the yield to 14.9 g/l.…”

Section: Discussionmentioning

confidence: 99%

Scaling Up for the Industrial Production of Rifamycin B Fed-Batch Production Mode in Shake Flasks and Bench-Scale Fermentor

Mohammad¹

2012

Fermentat Technol

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The production of rifamycin B using the gene amplified variant of Amycolatopsis mediterranei (NCH) was initially optimized in shake flasks through medium modifications and fed-batch addition of uracil. The yield was increased by 21.7% (from 11.7 to 14.3 g/l) when F2m1 medium was used. The production was further verified and optimized in fedbatch-mode in a laboratory fermentor using F2m3 medium and the optimized conditions (agitation 500 rpm, aeration; 1.5 for 3 days then control DO at 30% thereafter, pH; 6.5 for 3 days then 7 thereafter and control temperature at 28°C). Fed-batching of glucose syrup (5% v/v at day 3) and glucose (1% at days 6 and 8) increased the yield from 17.8 to 20.9 g/l (17.3%) at day 10. A yield of upto 20 g/l was recorded when 0.1% uracil was fed-batched at day 2. Integration of the most optimum conditions for fed-batching glucose syrup, glucose and uracil further increased the yield from 17.8 to 24.8 g/l (39%) in 10 days. The overall optimization of rifamycin B production increased the yield almost 2 folds. Statistical analysis revealed that there is a significant increase in rifamycin B production by using One-Way ANOVA at p<0.05 in all the tested fed-batch addition regimes.

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Improvement of rifamycin B production using mutant strains of Amycolatopsis mediterranei

Cited by 9 publications

References 13 publications

Optimization of Antimicrobial Production by a Marine Actinomycete Streptomyces afghaniensis VPTS3-1 Isolated from Palk Strait, East Coast of India

Optimization of Antimicrobial Production by a Marine Actinomycete Streptomyces afghaniensis VPTS3-1 Isolated from Palk Strait, East Coast of India

Optimization of rifamycin B fermentation in shake flasks via a machine‐learning‐based approach

Scaling Up for the Industrial Production of Rifamycin B Fed-Batch Production Mode in Shake Flasks and Bench-Scale Fermentor

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