Improvement of sciatic nerve regeneration by multichannel nanofibrous membrane-embedded electro-conductive conduits functionalized with laminin

Nazeri, Niloofar; Derakhshan, Mohammad Ali; Mansoori, Korosh; Ghanbari, Hossein

doi:10.1007/s10856-022-06669-0

Cited by 10 publications

(10 citation statements)

References 59 publications

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“…For instance, Nazeri et al designed a multichannel nerve conduit employing longitudinally aligned laminin-coated poly(lactic-co-glycolic acid) (PLGA)/carbon nanotubes (CNT) nanofibers within the lumen and randomly oriented PCL nanofibers on the outer surface, leading to supported SC migration and axon extension. 31 Shang et al developed conductive composite films of polypyrrole (PPy) doped with sodium dodecyl benzenesulfonate and GO through electrochemical deposition of PPy nanoparticles on aligned poly-L-lactide (PLLA) fibers, thereby facilitating nerve tissue growth. 32 Zhang et al crafted conduits featuring surface-anchored GO nanosheets on micropatterned poly(D,L lactide-co-caprolactone).…”

Section: Discussionmentioning

confidence: 99%

Oriented Graphene Oxide Scaffold Promotes Nerve Regeneration in vitro and in vivo

Zhou,

Tang,

Xiong

et al. 2024

IJN

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Background Treating peripheral nerve injuries (PNI) with defects remains challenging in clinical practice. The commercial conduits have shown suboptimal nerve regeneration and functional recovery due to their basic tubular design without electroactive and oriented topographical cues. Purpose To develop a new scaffold with oriented microstructure and electroactive Graphene oxide (GO) and investigate its’ therapeutic effect on nerve regeneration in vitro and in vivo. Methods This study employed a straightforward approach to co-spin PCL and GO, yielding an oriented hybrid nanofibrous scaffold known as the O-GO/PCL scaffold. The physical and chemical properties of nanofibrous scaffold were tested by scanning electron microscopy (SEM), transmission electron microscope (TEM), tensile test and so on. Primary Schwann cells (SCs) and dorsal root ganglia (DRG) were used to investigate the impact of the newly developed scaffolds on the biological behavior of neural cells in vitro. Transcriptome sequencing (mRNA-seq) was employed to probe the underlying mechanisms of the synergistic effect of electroactive GO and longitudinal topographic guidance on nerve regeneration. Furthermore, the developed O-GO/PCL scaffold was utilized to bridge a 10-mm sciatic nerve defect in rat, aiming to investigate its therapeutic potential for peripheral nerve regeneration in vivo. Results and discussion The SEM and TEM revealed that the newly developed O-GO/PCL scaffold showed longitudinally oriented microstructure and GO particles were homogenously and uniformly distributed inside the nanofibers. Primary SCs were utilized to assess the biocompatibility of the GO-based scaffold, revealing that negligible cytotoxicity when GO concentration does not exceed 0.5%. In vitro analysis of nerve regeneration demonstrated that axons in the O-GO/PCL group exhibited an average length of 1054.88 ± 161.32 µm, significant longer than those in the other groups ( P < 0.05). Moreover, mRNA sequencing results suggested that the O-GO/PCL scaffold could enhance nerve regeneration by upregulating genes associated with neural regeneration, encompassing ion transport, axon guidance and cell–cell interactions. Most importantly, we employed the O-GO/PCL scaffold to repair a 10-mm sciatic nerve defect in rat, resulting in augmented nerve regeneration, myelination, and functional recovery. Conclusion The O-GO/PCL scaffold with oriented microstructure and electroactive GO represents a promising heral nerve reconstruction.

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Section: Discussionmentioning

confidence: 99%

Oriented Graphene Oxide Scaffold Promotes Nerve Regeneration in vitro and in vivo

Zhou,

Tang,

Xiong

et al. 2024

IJN

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“…The study involved bridging a 10 mm defect in rats using the conduit and measuring nerve regeneration. They suggested that the use of multichannel structures in nerve conduits, specifically with a PLGA/CNT nanofiber design, holds promise for improving nerve regeneration [ 199 ].…”

Section: Cnt-incorporated Nanofibers For Tissue Engineeringmentioning

confidence: 99%

Biomedical Applications of CNT-Based Fibers

Jeong,

Kwon,

Shin

et al. 2024

Biosensors

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Carbon nanotubes (CNTs) have been regarded as emerging materials in various applications. However, the range of biomedical applications is limited due to the aggregation and potential toxicity of powder-type CNTs. To overcome these issues, techniques to assemble them into various macroscopic structures, such as one-dimensional fibers, two-dimensional films, and three-dimensional aerogels, have been developed. Among them, carbon nanotube fiber (CNTF) is a one-dimensional aggregate of CNTs, which can be used to solve the potential toxicity problem of individual CNTs. Furthermore, since it has unique properties due to the one-dimensional nature of CNTs, CNTF has beneficial potential for biomedical applications. This review summarizes the biomedical applications using CNTF, such as the detection of biomolecules or signals for biosensors, strain sensors for wearable healthcare devices, and tissue engineering for regenerating human tissues. In addition, by considering the challenges and perspectives of CNTF for biomedical applications, the feasibility of CNTF in biomedical applications is discussed.

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“…As a result, functional coatings have been applied on PLGA scaffolds to increase cell adhesion and viability. These coating materials include polymers such as polyethylene glycol (PEG) [ 7 ], polydopamine (PDA), polypyrrole (Ppy) [ 8 ], poly-lysine [ 9 ], polyethyleneimine (PEI) [ 10 ], and ECM components such as HA [ 11 ], laminin [ 12 ], FN [ 13 ], and collagen [ 14 ]. Studies have shown that modifying PLGA-based scaffolds with these materials could improve the hydrophilicity and cell adhesion on PLGA.…”

Section: Introductionmentioning

confidence: 99%

Surface Modification Progress for PLGA-Based Cell Scaffolds

Yan,

Hua,

Wang

et al. 2024

Polymers

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Poly(lactic-glycolic acid) (PLGA) is a biocompatible bio-scaffold material, but its own hydrophobic and electrically neutral surface limits its application as a cell scaffold. Polymer materials, mimics ECM materials, and organic material have often been used as coating materials for PLGA cell scaffolds to improve the poor cell adhesion of PLGA and enhance tissue adaptation. These coating materials can be modified on the PLGA surface via simple physical or chemical methods, and coating multiple materials can simultaneously confer different functions to the PLGA scaffold; not only does this ensure stronger cell adhesion but it also modulates cell behavior and function. This approach to coating could facilitate the production of more PLGA-based cell scaffolds. This review focuses on the PLGA surface-modified materials, methods, and applications, and will provide guidance for PLGA surface modification.

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Improvement of sciatic nerve regeneration by multichannel nanofibrous membrane-embedded electro-conductive conduits functionalized with laminin

Cited by 10 publications

References 59 publications

Oriented Graphene Oxide Scaffold Promotes Nerve Regeneration in vitro and in vivo

Oriented Graphene Oxide Scaffold Promotes Nerve Regeneration in vitro and in vivo

Biomedical Applications of CNT-Based Fibers

Surface Modification Progress for PLGA-Based Cell Scaffolds

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