Improvement of survival of skin flaps by combined gene transfer of hepatocyte growth factor and prostacyclin synthase

Abstract

Overall, these results indicate that combination treatment with HGF and PGIS genes by Shima Jet could be an effective strategy to improve skin flap survival.

Angiogenic Gene-Modified Fibroblasts for Induction of Localized Angiogenesis

“…Naked pDNA shows little efficiency when used for in vivo gene therapy. This is mainly due to rapid degradation by the host's enzymes as well as difficulties embedded in penetration of the polar molecule into the cell surrounded by an hydrophobic membrane [23]. Several plasmid carriers have been investigated to address this problem, among them polyethylene-imine (PEI).…”

Hypoxia upregulates PGI-synthase and increases PGI2 release in human vascular cells exposed to inflammatory stimuli

“…PGI 2 and PGE 2 are the main prostaglandins produced by human vascular cells ( 12 ). PGI 2 plays an important role in vascular biology in preventing thrombosis, acting as a potent vasorelaxant agent, and preserving endothelial cell viability (35)(36)(37). PGE 2 is a pro-infl ammatory molecule that is able to act through four different receptor subtypes.…”

“…by guest, on May 12, 2018 www.jlr.org Downloaded from cytokine toxicity ( 35,37 ). Therefore, PGI 2 seems to play a cytoprotective role under ischemic and infl ammatory conditions.…”

Use of gene-modified keratinocytes and fibroblasts to enhance regeneration in a full skin defect

“…Therefore, in this study we employed both VEGF and bFGF expressing Fb. This combined growth factor expression strategy also promoted epidermal regeneration [31]. Three weeks after transplantation of the scaffold seeded with modified cells, the histological analysis showed a fully differentiated human epithelium with all layers of normal epidermis.…”