Improvement of the mechanical properties of chitosan films by the addition of poly(ethylene oxide)

Alexeev, V.L.; Kel'berg, E. A.; Evmenenko, Guennadi; Bronnikov, Sergei

doi:10.1002/pen.11248

Cited by 63 publications

(35 citation statements)

References 11 publications

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“…55 Crosslinking is used to enhance mechanical strength and chemical stability, to control aqueous permeability and solubility of the hydrogels, and to decrease the aqueous swelling features of chitosan-based materials. [56][57][58][59][60][61] Crosslinking of the chitosan component with genipin enabled the creation of hydrogels with varying substrate stiffness, without changing the polysaccharide constituents. Results demonstrated that material properties (gel stiffness) can be leveraged to induce osteoblast differentiation in 2D culture as an alternative to biochemical cues such as soluble supplements, immobilized biomolecules and vectors, which are often expensive, labile and potentially carcinogenic.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Multifunctional Polysaccharide Hydrogels with Varying Stiffness for Bone Tissue Engineering

Pandit

Zuidema

Venuto

et al. 2013

Tissue Engineering Part A

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The use of hydrogels for bone regeneration has been limited due to their inherent low modulus to support cell adhesion and proliferation as well as their susceptibility to bacterial infections at the wound site. To overcome these limitations, we evaluated multifunctional polysaccharide hydrogels of varying stiffness to obtain the optimum stiffness at which the gels (1) induce proliferation of human dermal fibroblasts, human umbilical vascular endothelial cells (HUVECs), and murine preosteoblasts (MC3T3-E1), (2) induce osteoblast differentiation and mineralization, and (3) exhibit an antibacterial activity. Rheological studies demonstrated that the stiffness of hydrogels made of a polysaccharide blend of methylcellulose, chitosan, and agarose was increased by crosslinking the chitosan component to different extents with increasing amounts of genipin. The gelation time decreased (from 210 to 60 min) with increasing genipin concentrations. Proliferation of HUVECs decreased by 10.7 times with increasing gel stiffness, in contrast to fibroblasts and osteoblasts, where it increased with gel stiffness by 6.37 and 7.8 times, respectively. At day 14 up to day 24, osteoblast expression of differentiation markers-osteocalcin, osteopontin-and early mineralization marker-alkaline phosphatase, were significantly enhanced in the 0.5% (w/v) crosslinked gel, which also demonstrated enhanced mineralization by day 25. The antibacterial efficacy of the hydrogels decreased with the increasing degree of crosslinking as demonstrated by biofilm formation experiments, but gels crosslinked with 0.5% (w/v) genipin still demonstrated significant bacterial inhibition. Based on these results, gels crosslinked with 0.5% (w/v) genipin, where 33% of available groups on chitosan were crosslinked, exhibited a stiffness of 502 -64.5 Pa and demonstrated the optimal characteristics to support bone regeneration.

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Section: Discussionmentioning

confidence: 99%

Evaluation of Multifunctional Polysaccharide Hydrogels with Varying Stiffness for Bone Tissue Engineering

Pandit

Zuidema

Venuto

et al. 2013

Tissue Engineering Part A

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“…From this point of view, a research interest has been placed on blending of CS with poly(ethylene oxide) (PEO). [1][2][3][4][5] CS/PEO blends exhibit the improvement in thermal, mechanical and electrical properties because of the specific interactions between the amine and hydroxyl groups of CS and the ether groups of PEO [1][2][3][4] , as naturally expected from their chemical structures shown later in Fig.1. The CS/PEO blends have a potential applicability as a matrix in the solid polymer electrolyte [6][7][8] , because both CS and PEO can dissolve salts to exhibit ionic conductivity.…”

Section: Introductionmentioning

confidence: 91%

Dielectric Relaxation and Ionic Conductivity of a Chitosan/Poly(ethylene oxide) Blend Doped with Potassium and Calcium Cations

Rakkapao

Watanabe

Masubuchi

2016

J. Soc. Rheol., Jpn

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“…Residue of PEO in animal tissue normally induced few immune responses owing to its extremely low toxicity. 28,29 Moreover, composites of PEO in biomimetic scaffolds favored the formation of neocartilage. 30 Recently, influences of the composition on porosity and mechanical strength of PEO/chitin/chitosan scaffolds and applications of the ternary scaffolds to cartilage tissue engineering were investigated.…”

Section: Introductionmentioning

confidence: 99%

Application of polyethyleneimine‐modified scaffolds to the regeneration of cartilaginous tissue

Kuo

2009

Biotechnology Progress

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In this study, we analyzed the physicochemical and biophysical properties of three-dimensional scaffolds modified using polyethyleneimine (PEI) and applied these scaffolds to the cultivation of bovine knee chondrocytes (BKCs). PEI was crosslinked in the bulk or on the surface of the ternary scaffolds comprising polyethylene oxide, chitin and chitosan. The results revealed that when the concentration of PEI was less than 300 microg/mL, the cytotoxicity of a scaffold was on the same order in the two method of modification. An increase in the concentration of PEI favored the adhesion of BKCs. When the amount of PEI in scaffolds is fixed, the surface-modified scaffolds exhibited a higher adhesion efficiency of BKCs than the bulk-modified scaffolds. For the regeneration of cartilaginous components, a higher amount of PEI in a scaffold yielded larger amounts of proliferated BKCs, secreted glycosaminoglycans, and produced collagen. In addition, the formation of neocartilage in the surface-modified scaffolds was more effective than that in the bulk-modified scaffolds. These tissue-engineered scaffolds, modified by an appropriate concentration of PEI, can be potentially applied to cartilage repair in clinical trials.

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Improvement of the mechanical properties of chitosan films by the addition of poly(ethylene oxide)

Cited by 63 publications

References 11 publications

Evaluation of Multifunctional Polysaccharide Hydrogels with Varying Stiffness for Bone Tissue Engineering

Evaluation of Multifunctional Polysaccharide Hydrogels with Varying Stiffness for Bone Tissue Engineering

Dielectric Relaxation and Ionic Conductivity of a Chitosan/Poly(ethylene oxide) Blend Doped with Potassium and Calcium Cations

Application of polyethyleneimine‐modified scaffolds to the regeneration of cartilaginous tissue

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