Improvement of the potency of microalgae Nannochloropsis and Chaetoceros through antioxidant analysis and optimization of DNA isolation

Zulkarnain, M I; Rahayu, Hastini; Herida, A P; Erfianti, Tia; Kusumaningrum, Hermin Pancasakti; Zainuri, Muhammad; Endrawati, Hadi; Widowati, Ita; Haryanti, Wiworo; Mahendrajaya, Robertus Triaji

doi:10.1088/1742-6596/1943/1/012080

Cited by 3 publications

(2 citation statements)

References 15 publications

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“…For example, Nannochloropsis and Chaetoceros are ubiquitous microalgae used as natural feed for aquaculture animals. In addition, these microalgae can produce secondary metabolites utilized as antioxidants (Zulkarnain et al, 2020). Previous research by Tunio et al (2022) also reported that cyanobacteria Oscilatoria limosa produced some bioactive compounds including phenolic acid, flavonoid, proteins, amino acid, and sugar.…”

Section: Introductionmentioning

confidence: 99%

Tocopherol Content of Euglena sp. Isolated from Yogyakarta under Glucose and Ethanol Mixture Treatment

AQİLLA,

ANDESKA,

ERFİANTİ

et al. 2023

Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi

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Euglena sp. is a microalgae with significant potential for utilization as a high-value product because of the presence of protein, lipid, paramylon, and other compounds. Even though these microalgae may be found in freshwater, research on enhancing Euglena sp. cultivation is still limited in Indonesia. Tocopherols are antioxidants that can effectively protect against diseases caused by oxidative stress. The isomer of tocopherol with the highest biological activity is α-tocopherol. Euglena sp. cells had the highest levels of α-tocopherol compared to other microorganisms. Scientists are continuously trying to determine how to obtain a high α-tocopherol concentration and a significant Euglena cell biomass. Photosynthetic organisms culture has been found to boost α-tocopherol content in Euglena sp., although heterotrophic culture can potentially increase biomass. This study used photoheterotrophic culture with a mixture of glucose and ethanol to increase the α-tocopherol and biomass concentration inside the culture of the local strain of Euglena sp. The addition of treatments in a glucose and ethanol combination with levels of 3:2; 2.5: 2.5; 2: 2; and 0:0 (control) g L-1 was used in this study to assess the impact of Euglena sp. culture on growth, biomass, and α-tocopherol concentration. According to the findings of this study, the 3:2 treatment produced the most significant specific growth rate and biomass, including 0.992 (OD680/OD680/day) and 8.480 (g L-1). In contrast, the 2.5:2.5 treatment produced the highest α-tocopherol content, specifically 7.09±0.096 mg L-1.

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Section: Introductionmentioning

confidence: 99%

Tocopherol Content of Euglena sp. Isolated from Yogyakarta under Glucose and Ethanol Mixture Treatment

AQİLLA,

ANDESKA,

ERFİANTİ

et al. 2023

Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi

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“…Currently, there is unexploited biodiversity in Indonesia, particularly microorganisms that play an essential function for humans (Erfianti et al, 2021;Irawan et al, 2023), including microalgae. Microalgae are micro-cell factories that can produce useful compounds such as protein, lipids, carbohydrates, and antioxidants (Zulkarnain et al, 2021). Euglena sp.…”

Section: Introductionmentioning

confidence: 99%

Growth and Metabolite Enhancement of Acidophile Euglena sp. Isolated from Indonesia under Different Photoperiod Cycles

Erfianti,

Daryono,

Budiman

et al. 2023

JIPK

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Euglena sp. is a unicellular, flagellated microalga considered one of the most promising microalgal feedstock species for biofuels. Reducing the level of liquid waste pollutants can be done biologically by using microalgal organisms. Its metabolites, including lipids, proteins, carbohydrates, and pigments, are appropriate for producing biorefinery products such as biodiesel and jet fuels. They can be isolated from extreme environments, such as highly acidic and ammonia-rich environments, that are not conducive to their proliferation. This study sought to determine the effect of the photoperiod or (light: dark) cycle (24 L:0 D, 12 L:12 D, 14 L:10 D, and 16 L:8 D) on the growth, biomass, metabolite content consisting of lipids, carbohydrates, and proteins, and the rate of CO2 uptake by Euglena sp. As stated previously, the study was conducted by cultivating Euglena sp. on a laboratory scale with four photoperiod regimens. The results indicated that optimal growth, biomass content, and metabolite content were obtained with a 24 D:0 L lighting cycle. The control treatment (24 L: 0 D) had the highest biomass productivity (0.032 g.L-1.day-1 ± 0.004), lipid content (0.387 g.L-1 ± 0.031), protein content (0.542 mg.Ml-1 ± 0.007), carbohydrate content (0.409 x104 g.L-1), chlorophyll a (6.237 g.L-1 ± 0.184), chlorophyll b (2.838 g.L-1 ± 0.253), and total carotenoid (1.566 g.L-1 ± 0.105). Full light illumination (24 L:0 D) was significantly producing carotenoid content, including phaeophytin a, phaeophytin b, violaxanthin, 9’-cis-neoxanthin, dino xanthin, and fucoxanthin. Highlight Research The growth rate was higher when Euglena was cultivated under continuous illumination. The biomass productivity of Euglena increased significantly under continuous illumination. The metabolite content of Euglena (lipids, proteins, and pigments) was higher in continuous illumination.

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Comparison of CO2 absorption via terrestrial plants and microalgae: A review

Erfianti,

Nurafifah,

Sadewo

et al. 2024

APJMBB

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Currently, global warming and climate change continue to increase along with CO2 gas emissions. This has an impact on the survival of organisms, including humans. Therefore, efforts to reduce CO2 emissions have been conducted by various methods, such as chemical, physical and biological methods, one of the most efficient methods to absorb CO2 gas is to use microalgae. Microalgae are photosynthetic organisms capable of absorbing CO2. Microalgae can also be converted into valuable products such as biofuels, biofertilizers, food, feed, medicines, and cosmetics through an integrated biorefinery concept. In the future, CO2 mitigation using microalgae will be massively studied, considering the many benefits obtained from the utilization of microalgae to reduce CO2 emissions in the world. Through the concept of biorefinery, microalgae can be processed into various derivative products that are useful for humans in the food, feed, health, industrial, medicine, and cosmetic sectors. This review will compare the effectiveness of CO2 absorption through terrestrial plants, microalgae, and microalgae-bacterial consortia to the possibility of its application and challenges.

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Improvement of the potency of microalgae Nannochloropsis and Chaetoceros through antioxidant analysis and optimization of DNA isolation

Cited by 3 publications

References 15 publications

Tocopherol Content of Euglena sp. Isolated from Yogyakarta under Glucose and Ethanol Mixture Treatment

Tocopherol Content of Euglena sp. Isolated from Yogyakarta under Glucose and Ethanol Mixture Treatment

Growth and Metabolite Enhancement of Acidophile Euglena sp. Isolated from Indonesia under Different Photoperiod Cycles

Comparison of CO2 absorption via terrestrial plants and microalgae: A review

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